Results 61 to 70 of about 5,306,183 (290)

Lipase activity in vesicular systems: Characterization of candida cylindracea lipase and its activity in polymerizable dialkylammonium surfactant vesicles [PDF]

open access: yes, 1993
Lipase from Candida cylindracea (CCL) was incorporated into polymerizable positively charged dialkylammonium bromide surfactant vesicles. The enzyme was incorporated by the use of the dehydration-rehydration method or by incubation.
Engbersen, J.F.J.   +2 more
core   +4 more sources

Kesesuaian Hasil Pemeriksaan Antibodi Virus Herpes Simpleks Metode Enzyme-Linked Immunofiltration Assay dengan Enzyme-Linked Immunosorbent Assay

open access: yesMajalah Kedokteran Bandung, 2012
Herpes simplex virus (HSV) infections are very common and are caused by HSV type 1 (HSV-1) and HSV type 2 (HSV-2). HSV-1 being mostly associated with orofacial disease, whereas HSV-2 is usually associated with perigenital infection.
Victor Immanuel   +2 more
doaj   +1 more source

Comparison of Oxygen Electrode Chronoamperometry and Spectrophotometry for Determination of Catalase Activity

open access: yesOxygen, 2023
Catalase is a key antioxidative enzyme, and a deficiency or malfunction of catalase is hypothesized to be related to various diseases. To investigate catalase activity, it is important to use reliable methods and experimental protocols enabling ...
Michal Szczepanczyk   +3 more
doaj   +1 more source

SARS‐CoV‐2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup

open access: yesCurrent Protocols in Microbiology, 2020
In late 2019, cases of atypical pneumonia were detected in China. The etiological agent was quickly identified as a betacoronavirus (named SARS‐CoV‐2), which has since caused a pandemic.
D. Stadlbauer   +15 more
semanticscholar   +1 more source

Enzyme-linked immunosorbent assay for urinary albumin at low concentrations [PDF]

open access: yes, 1987
We describe an enzyme-linked immunosorbent assay (ELISA) for urinary albumin. It requires only commercially available reagents, can detect as little as 16 micrograms of albumin per liter, and analytical recovery ranges from 92 to 116%.
Jesse, U.   +4 more
core  

Cloning and Overexpression of Strictosidine β-D-Glucosidase Gene Short Sequence from Catharanthus roseus in Escherichia coli [PDF]

open access: yesAdvanced Pharmaceutical Bulletin, 2019
Purpose: Strictosidine-β-D-glucosidase (SGD) is considered as a key enzyme in the production of bisindole alkaloids in Catharanthus roseus. The present study illustrated the production of a short sequence of this enzyme in Escherichia coli without codon ...
Ahmed Saeed Arafa   +4 more
doaj   +1 more source

A fast, sensitive and fluorescent LPMO activity assay

open access: yesFrontiers in Microbiology, 2023
Lytic polysaccharide monooxygenases (LPMOs) are industrially relevant enzymes that utilize a copper co-factor and an oxygen species to break down recalcitrant polysaccharides.
Johan Ø. Ipsen   +2 more
doaj   +1 more source

An Inhibitive Enzyme Assay to Detect Mercury and Zinc Using Protease from Coriandrum sativum

open access: yesTheScientificWorldJournal, 2013
Heavy metals pollution has become a great threat to the world. Since instrumental methods are expensive and need skilled technician, a simple and fast method is needed to determine the presence of heavy metals in the environment.
G. Baskaran   +3 more
semanticscholar   +1 more source

Adaptation of a Commercial NAD+ Quantification Kit to Assay the Base-Exchange Activity and Substrate Preferences of SARM1

open access: yesMolecules
Here, we report an adapted protocol using the Promega NAD/NADH-Glo™ Assay kit. The assay normally allows quantification of trace amounts of both oxidized and reduced forms of nicotinamide adenine dinucleotide (NAD) by enzymatic cycling, but we now show ...
Ilenia Cirilli   +4 more
doaj   +1 more source

Cloning, expression, purification and assay of sorbitol dehydrogenase from "Feicheng" peach fruit (Prunus persica)

open access: yesBrazilian Archives of Biology and Technology, 2013
A gene encoding NAD+-dependent sorbitol dehydrogenase (SDH) in peach fruit was cloned and expressed in Escherichia coli. Recombinant SDH protein with 6×His-tagged was localized exclusively in the cytoplasmic soluble fraction of E.
Zhen Sun   +3 more
doaj   +1 more source

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