Results 81 to 90 of about 5,306,282 (389)
Universal buffers for use in biochemistry and biophysical experiments
AIMS Biophysics, 2015 The use of buffers that mimic biological solutions is a foundation of biochemical and biophysical studies. However, buffering agents have both specific and nonspecific interactions with proteins.
Dewey Brooke +2 more
doaj +1 more source
CRISPR Enzyme Kinetics for Molecular Diagnostics.
Analytical Chemistry, 2021 CRISPR-diagnostic assays have gained significant interest in the last few years. This interest has grown rapidly during the current COVID-19 pandemic, where CRISPR-diagnostics have been frontline contenders for rapid testing solutions.
A. Ramachandran, J. Santiago
semanticscholar +1 more source
The Development of a Direct Homologous Radioimmunoassay for Serum Cortisol [PDF]
, 1981 Peer ...
Giesemann, G. +4 more
core +2 more sources
FEBS Letters, EarlyView.The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp +5 more
wiley +1 more source
Biology and Life Sciences ForumThermostable amylases are among the most widely used and desirable enzymes in the food industry. Indeed, they guarantee faster reactions at high temperatures, enhanced substrate solubility and reduced microbial contamination and cooling costs.
Sarra Bouaita +4 more
doaj +1 more source
mBio, 2020 Methylglyoxal (MG) is a detrimental metabolic by-product that threatens most organisms (in humans MG causes diabetes). MG is predominantly detoxified by the glyoxalase pathway.
Xavier Kammerscheit +4 more
doaj +1 more source
European Journal of Biochemistry, 1974 The autoxidation of pyrogallol was investigated in the presence of EDTA in the pH range 7.9–10.6. The rate of autoxidation increases with increasing pH.
S. Marklund, Gudrun Marklund
semanticscholar +1 more source
Purification and properties of a HeLa cell enzyme able to remove the 5'- terminal protein from poliovirus RNA [PDF]
, 1980 Using a rapid phenol extraction assay, an enzyme was purified from uninfected HeLa cells that can cleave the 5'-terminal protein (VPg) from poliovirus RNA. Both cytoplasmic and nuclear extracts had enzymes with similar behavior.
Ambros, Victor, Baltimore, David
core
FEBS Letters, EarlyView.UDP‐glucuronic acid 4‐epimerase (UGAepi) catalyzes NAD+‐dependent interconversion of UDP‐glucuronic acid (UDP‐GlcA) and UDP‐galacturonic acid (UDP‐GalA) via C4‐oxidation, 4‐keto‐intermediate rotation, and C4‐reduction. Here, Borg et al. examined the role of the substrate's carboxylate group in the enzymic mechanism by analyzing NADH‐dependent reduction
Annika J. E. Borg +2 more
wiley +1 more source
Kinetic modelling of in vitro data of PI3K, mTOR1, PTEN enzymes and on-target inhibitors Rapamycin, BEZ235, and LY294002 [PDF]
, 2016 The phosphatidylinositide 3-kinases (PI3K) and mammalian target of rapamycin-1 (mTOR1) are two key targets for anti-cancer therapy. Predicting the response of the PI3K/AKT/mTOR1 signalling pathway to targeted therapy is made difficult because of network ...
Bown, James L. +4 more
core +3 more sources