Results 11 to 20 of about 4,824,034 (339)

Rational Design for Enhanced Acyltransferase Activity in Water Catalyzed by the Pyrobaculum calidifontis VA1 Esterase

open access: yesMicroorganisms, 2021
Biocatalytic transesterification is commonly carried out employing lipases in anhydrous organic solvents since hydrolases usually prefer hydrolysis over acyl transfer in bulk water.
Amanda Staudt   +7 more
doaj   +1 more source

New gold pincer-type complexes induce caspase-dependent apoptosis in human cancer cells in vitro [PDF]

open access: yesVojnosanitetski Pregled, 2021
Background/Aim. The use of cisplatin as a chemotherapeutic opened the door to the new metal-based drug research. New complexes containing metals such as platinum, palladium, ruthenium and gold have recently been analyzed as potential antitumor agents ...
Zarić Milan M.   +9 more
doaj   +1 more source

Using Steady-State Kinetics to Quantitate Substrate Selectivity and Specificity: A Case Study with Two Human Transaminases

open access: yesMolecules, 2022
We examined the ability of two human cytosolic transaminases, aspartate aminotransferase (GOT1) and alanine aminotransferase (GPT), to transform their preferred substrates whilst discriminating against similar metabolites.
Alessio Peracchi, Eugenia Polverini
doaj   +1 more source

Monitoring Protein Denaturation of Egg White Using Passive Microwave Radiometry (MWR)

open access: yesDiagnostics, 2022
Passive microwave radiometry (MWR) is a measurement technique based on the detection of passive radiation in the microwave spectrum of different objects.
Igor Goryanin   +3 more
doaj   +1 more source

Enzymatic Methods for Salivary Biomarkers Detection: Overview and Current Challenges

open access: yesMolecules, 2021
Early detection is a key factor in patient fate. Currently, multiple biomolecules have been recognized as biomarkers. Nevertheless, their identification is only the starting line on the way to their implementation in disease diagnosis.
Alonso Ornelas-González   +3 more
doaj   +1 more source

A review of phosphatidate phosphatase assays

open access: yesJournal of Lipid Research, 2020
Phosphatidate phosphatase (PAP) catalyzes the penultimate step in the synthesis of triacylglycerol and regulates the synthesis of membrane phospholipids.
Prabuddha Dey   +2 more
doaj   +1 more source

Growth amplification in ultrahigh-throughput microdroplet screening increases sensitivity of clonal enzyme assays and minimizes phenotypic variation.

open access: yesLab on a Chip, 2020
Microfluidic ultrahigh-throughput screening of enzyme activities provides information on libraries with millions of variants in a day. Each individual library member is represented by a recombinant single cell, compartmentalised in an emulsion droplet ...
P. Zurek   +4 more
semanticscholar   +1 more source

A simple and reliable method for determination of optimum pH in coupled enzyme assays.

open access: yesBioTechniques, 2020
Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes.
Lee Bowman   +7 more
semanticscholar   +1 more source

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