Results 21 to 30 of about 651,887 (304)

A new intrinsic thermal parameter for enzymes reveals true temperature optima [PDF]

, 2004
Two established thermal properties of enzymes are the Arrhenius activation energy and thermal stability. Arising from anomalies found in the variation of enzyme activity with temperature, a comparison has been made of experimental data for the activity ...
Daniel, Roy M., Danson, Michael J., Eisenthal, Robert, Peterson, Michelle E., Spence, Alastair   +4 more
core   +2 more sources

Synergistic action of the gut microbiota in environmental RNA interference in a leaf beetle

Microbiome, 2021
Background RNA interference (RNAi) has emerged as an efficient tool to control insect pests. When insects ingest double-stranded RNAs (dsRNAs) targeted against essential genes, strong gene silencing and mortality can be induced.
Letian Xu, Shijing Xu, Liuwei Sun, Yiqiu Zhang, Jing Luo, Ralph Bock, Jiang Zhang   +6 more
doaj   +1 more source

Phosphoenolpyruvate Carboxykinase as the Sole Anaplerotic Enzyme in Saccharomyces cerevisiae [PDF]

, 2010
Pyruvate carboxylase is the sole anaplerotic enzyme in glucose-grown cultures of wild-type Saccharomyces cerevisiae. Pyruvate carboxylase-negative (Pyc–) S.
Zelle, Rintze M., Trueheart, Josh, Harrison, Jacob C., Pronk, Jack T., van Maris, Antonius J. A., Van Maris, A. J. A.   +5 more
core   +1 more source

Laccase production and differential transcription of laccase genes in Cerrena sp. in response to metal ions, aromatic compounds and nutrients

Frontiers in Microbiology, 2016
Laccases can oxidize a wide range of aromatic compounds and are industrially valuable. Laccases often exist in gene families and may differ from each other in expression and function.
Jie eYang, Jie eYang, Guozeng eWang, Guozeng eWang, Tzi Bun eNg, Juan eLin, Juan eLin, Xiuyun eYe, Xiuyun eYe   +8 more
doaj   +1 more source

Proteolytic enzyme engineering : a tool for wool [PDF]

, 2009
One of the goals of protein engineering is to tailor the structure of enzymes to optimize industrial bioprocesses. In the present work, we present the construction of a novel high molecular weight subtilisin, based on the fusion of the DNA sequences ...
Araújo R., Banki M. R., Brack N., Girotti A., Herrero-Vanrell R., Hull M. E., Inoue H., Laemmli U. K., Levene R., Li B., Li B., Lowry O. H., Meyer D. E., Morihara K., Morihara K., Negri A. P., Parvinzadeh M., Power S. D., Rodríguez-Cabello J. C., Sambroock J., Sanger F., Schlink T., Shen J., Silva C. J. S. M., Silva C. J. S. M., Silva C. J. S. M., Tirrell D. A., Trabbic-Carlson K.   +27 more
core   +1 more source

A Broad-Specificity Chitinase from Penicillium oxalicum k10 Exhibits Antifungal Activity and Biodegradation Properties of Chitin

Marine Drugs, 2021
Penicillium oxalicum k10 isolated from soil revealed the hydrolyzing ability of shrimp chitin and antifungal activity against Sclerotinia sclerotiorum. The k10 chitinase was produced from a powder chitin-containing medium and purified by ammonium sulfate
Xing-Huan Xie, Xin Fu, Xing-Yu Yan, Wen-Fang Peng, Li-Xin Kang   +4 more
doaj   +1 more source

Analysis of a conserved cellulase transcriptional regulator reveals inducer-independent production of cellulolytic enzymes in Neurospora crassa. [PDF]

, 2013
Cellulose is recalcitrant to deconstruction to glucose for use in fermentation strategies for biofuels and chemicals derived from lignocellulose. In Neurospora crassa, the transcriptional regulator, CLR-2, is required for cellulolytic gene expression and
Coradetti, Samuel, Glass, N. Louise, Xiong, Yi   +2 more
core   +2 more sources

A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells

Communications Biology, 2021
Wang et al. developed a fluorescence-assisted single-cell methodology (split protease-E. coli ClpXP (SPEC)) to characterise protein-protein interactions for both eukaryotic and prokaryotic species in E. coli cells.
Shengchen Wang, Faying Zhang, Meng Mei, Ting Wang, Yueli Yun, Shihui Yang, Guimin Zhang, Li Yi   +7 more
doaj   +1 more source

Research Progress on Fungal Sesterterpenoids Biosynthesis

Journal of Fungi, 2022
Sesterterpenes are 25-carbon terpenoids formed by the cyclization of dimethyl allyl diphosphate (DMAPP) and isopentenyl diphosphate (IPP) as structural units by sesterterpenes synthases.
Ping Zhang, Jianzhao Qi, Yingce Duan, Jin-ming Gao, Chengwei Liu   +4 more
doaj   +1 more source

Directed enzyme evolution: climbing fitness peaks one amino acid at a time [PDF]

, 2009
Directed evolution can generate a remarkable range of new enzyme properties. Alternate substrate specificities and reaction selectivities are readily accessible in enzymes from families that are naturally functionally diverse.
Arnold, Frances H., Tracewell, Cara A.
core   +2 more sources