Results 41 to 50 of about 5,705,534 (301)

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

Frontiers in Microbiology, 2017
Laccases are a family of copper-containing oxidases with important applications in bioremediation and other various industrial and biotechnological areas.
Jie Yang, Wenjuan Li, Tzi Bun Ng, Xiangzhen Deng, Juan Lin, Xiuyun Ye   +5 more
doaj   +1 more source

Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant growth enhancer and GlcNAc

Microbial Cell Factories, 2020
Chitinase and chitin-oligosaccaride can be used in multiple field, so it is important to develop a high-yield chitinase producing strain. Here, a recombinant Pichia pastoris with 4 copies of ChiA gene from Bacillus licheniformis and co-expression of ...
Wen Song, Nuo Zhang, Mo Yang, Yuling Zhou, Nisha He, Guimin Zhang   +5 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Design of minibinder proteins as universal antagonists against canine and human TNFα

Communications Biology
In this study, we resolve the complex structure of canine TNFα (cTNFα) trimer bound to nanobody molecules at a resolution of 3.1 Å using cryo-electron microscopy.
Jun Weng, Zhiyong Wu, Banbin Xing, Yang Hu, Xiaoyu Hu, Meng Mei, Jiaxin Xu, Mengqing Lu, Yibin Chen, Lin Wei, Ke Ming, Zhizheng Wang, Zhuang Li, Zigong Wei   +13 more
doaj   +1 more source

Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins

Microbial Cell Factories, 2019
Background The methylotrophic yeast Pichia pastoris is well-known for the production of a broad spectrum of functional types of heterologous proteins including enzymes, antigens, engineered antibody fragments, and next gen protein scaffolds and many ...
Xueyun Zheng, Yimin Zhang, Xinying Zhang, Cheng Li, Xiaoxiao Liu, Ying Lin, Shuli Liang   +6 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Decoupling enzyme catalysis from thermal denaturation [PDF]

, 2010
The equilibrium model (EM) (Daniel et al., 2001) postulates two forms of a folded enzyme, one catalytically active (Eact) and the other inactive (Einact), which interconvert via a fast thermal equilibrium (Keq) (Figure A). This model for enzyme catalysis
Easter, Ashley Davys
core  

Enzyme Engineering for In Situ Immobilization [PDF]

Molecules, 2016
Enzymes are used as biocatalysts in a vast range of industrial applications. Immobilization of enzymes to solid supports or their self-assembly into insoluble particles enhances their applicability by strongly improving properties such as stability in changing environments, re-usability and applicability in continuous biocatalytic processes.
Rehm, Fabian BH, Chen, Shuxiong, Rehm, Bernd HA   +2 more
openaire   +4 more sources

A new strategy for cellulases application in high temperature industrial scenarios with syringic acid assisting

GCB Bioenergy, 2023
In the process of bioethanol production, more stable and active cellulase in high temperature condition is required. In this study, syringic acid was applied in cellulase hydrolysis system.
Qiuping Ran, Mengtian Huang, Huanan Li, Bixin Chen, Lili He, Xinxin Cui, Jiashu Liu, Zhengbing Jiang   +7 more
doaj   +1 more source