Results 41 to 50 of about 365,104 (302)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

A new phosphatase of the haloacid dehalogenase superfamily from the hyperthermophilic archaeon Thermococcus onnurineus NA1 [PDF]

, 2007
As a part of genomic research of the Thermococcus onnurineus NA1, a hyperthermophilic archaeon collected from a deep-sea hydrothermal vent area, a gene annotated as a hydrolase of the haloacid dehalogenase superfamily was investigated to assign its ...
이정현, 강성균, 조요나, 이현숙   +3 more
core  

Laccases: Production, Expression Regulation, and Applications in Pharmaceutical Biodegradation

Frontiers in Microbiology, 2017
Laccases are a family of copper-containing oxidases with important applications in bioremediation and other various industrial and biotechnological areas.
Jie Yang, Wenjuan Li, Tzi Bun Ng, Xiangzhen Deng, Juan Lin, Xiuyun Ye   +5 more
doaj   +1 more source

Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant growth enhancer and GlcNAc

Microbial Cell Factories, 2020
Chitinase and chitin-oligosaccaride can be used in multiple field, so it is important to develop a high-yield chitinase producing strain. Here, a recombinant Pichia pastoris with 4 copies of ChiA gene from Bacillus licheniformis and co-expression of ...
Wen Song, Nuo Zhang, Mo Yang, Yuling Zhou, Nisha He, Guimin Zhang   +5 more
doaj   +1 more source

Engineering an enzyme to resist boiling [PDF]

Proceedings of the National Academy of Sciences, 1998
In recent years, many efforts have been made to isolate enzymes from extremophilic organisms in the hope to unravel the structural basis for hyperstability and to obtain hyperstable biocatalysts. Here we show how a moderately stable enzyme (a thermolysin-like protease from Bacillus stearothermophilus , TLP-ste) can ...
B, Van den Burg, G, Vriend, O R, Veltman, G, Venema, V G, Eijsink   +4 more
openaire   +2 more sources

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

'Dopamine-first' mechanism enables the rational engineering of the norcoclaurine synthase aldehyde activity profile. [PDF]

, 2015
Norcoclaurine synthase (NCS) (EC 4.2.1.78) catalyzes the Pictet-Spengler condensation of dopamine and an aldehyde, forming a substituted (S)-tetrahydroisoquinoline, a pharmaceutically important moiety.
Nicholas H. Keep, Markus C. Gershater, Helen C. Hailes, Ward, J.M., Pesnot, T, Eleanor D. Lamming, Pesnot, T., Thomas Pesnot, Hailes, HC, Gershater, M.C., Ward, JM, Lamming, E.D., Keep, Nicholas H., Altin Sula, Keep, NH, Hailes, H.C., Lichman, B.R., John M. Ward, Sula, Altin, Benjamin R. Lichman, Lichman, BR, Gershater, MC, Sula, A, Lamming, ED   +23 more
core   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Synthetic biology—putting engineering into biology [PDF]

, 2006
Synthetic biology is interpreted as the engineering-driven building of increasingly complex biological entities for novel applications. Encouraged by progress in the design of artificial gene networks, de novo DNA synthesis and protein engineering, we ...
Panke, Sven, Heinemann, Matthias,, Panke, Sven,, Heinemann, Matthias   +3 more
core   +1 more source

Design of minibinder proteins as universal antagonists against canine and human TNFα

Communications Biology
In this study, we resolve the complex structure of canine TNFα (cTNFα) trimer bound to nanobody molecules at a resolution of 3.1 Å using cryo-electron microscopy.
Jun Weng, Zhiyong Wu, Banbin Xing, Yang Hu, Xiaoyu Hu, Meng Mei, Jiaxin Xu, Mengqing Lu, Yibin Chen, Lin Wei, Ke Ming, Zhizheng Wang, Zhuang Li, Zigong Wei   +13 more
doaj   +1 more source