Results 61 to 70 of about 658,387 (301)

Flavoprotein monooxygenases for oxidative biocatalysis: recombinant expression in microbial hosts and applications [PDF]

, 2014
External flavoprotein monooxygenases comprise a group of flavin-dependent oxidoreductases that catalyze the insertion of one atom of molecular oxygen into an organic substrate and the second atom is reduced to water. These enzymes are involved in a great
Bianchi, Dario Alejandro, Ceccoli, Romina Denis, Rial, Daniela Veronica   +2 more
core   +2 more sources

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

The alkynyl-containing compounds from mushrooms and their biological activities

Natural Products and Bioprospecting, 2023
Mushrooms have been utilized by humans for thousands of years due to their medicinal and nutritional properties. They are a crucial natural source of bioactive secondary metabolites, and recent advancements have led to the isolation of several alkynyl ...
Ji-shuang Qi, Yingce Duan, Zhao-chen Li, Jin-ming Gao, Jianzhao Qi, Chengwei Liu   +5 more
doaj   +1 more source

Heterologous expression and functional characterization of a GH10 endoxylanase from \u3ci\u3eAspergillus fumigatus\u3c/i\u3e var. \u3ci\u3eniveus\u3c/i\u3e with potential biotechnological application [PDF]

, 2019
Xylanases decrease the xylan content in pretreated biomass releasing it from hemicellulose, thus improving the accessibility of cellulose for cellulases. In this work, an endo-β-1,4-xylanase from Aspergillus fumigatus var.
Abdella, Asmaa, da Silva Lima, Awana, Damasio, Andre, Ferreira Milagres, Adriane Maria, Goncalves, Aline Larissa, Goncalves, Thiago Augusto, Mulinari, Evandro Jose, Oliva, Bianca, Quintero, Leidy Patricia, Segato, Fernando, Squina, Fabio Marcio, Velasco, Josman, Wilkins, Mark R.   +12 more
core   +1 more source

Enzyme Engineering for In Situ Immobilization [PDF]

Molecules, 2016
Enzymes are used as biocatalysts in a vast range of industrial applications. Immobilization of enzymes to solid supports or their self-assembly into insoluble particles enhances their applicability by strongly improving properties such as stability in changing environments, re-usability and applicability in continuous biocatalytic processes.
Rehm, Fabian BH, Chen, Shuxiong, Rehm, Bernd HA   +2 more
openaire   +4 more sources

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

A Whole-Process Visible Strategy for the Preparation of Rhizomucor miehei Lipase with Escherichia coli Secretion Expression System and the Immobilization

Microbial Cell Factories
Background Rhizomucor miehei (RM) lipase is a regioselective lipase widely used in food, pharmaceutical and biofuel industries. However, the high cost and low purity of the commercial RM lipase limit its industrial applications.
Mingjun Yang, Xianhui Su, Jun Yang, Zhiwen Lu, Jie Zhou, Fei Wang, Yang Liu, Lixin Ma, Chao Zhai   +8 more
doaj   +1 more source

Tri©DB: an integrated platform of knowledgebase and reporting system for cancer precision medicine

Journal of Translational Medicine, 2023
Background With the development of cancer precision medicine, a huge amount of high-dimensional cancer information has rapidly accumulated regarding gene alterations, diseases, therapeutic interventions and various annotations.
Wei Jiang, Peng-Ying Wang, Qi Zhou, Qiu-Tong Lin, Yao Yao, Xun Huang, Xiaoming Tan, Shihui Yang, Weicai Ye, Yuedong Yang, Yun-Juan Bao   +10 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Preparation and characterization of protein-nanotube conjugates [PDF]

, 2009
This chapter describes methods of immobilizing proteins on carbon nanotubes, using two different routes—physical adsorption and covalent attachment.
Asuri, Prashanth, Bale, Shyam Sundhar, Banerjee, Akhilesh, Dordick, Jonathan S., Joshi, Amit, Kane, Ravi S., Pangule, Ravindra C., Shah, Dhiral A., Vance, David   +8 more
core   +1 more source