Results 71 to 80 of about 365,104 (302)

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Biocatalysis, Enzyme Engineering and Biotechnology [PDF]

, 2012
Enzymes are biocatalysts evolved in nature to achieve the speed and coordination of nearly all the chemical reactions that define cellular metabolism necessary to develop and maintain life.
D. Platis, I. A. Axarli, Labrou, N. E., E. G. Chronopoulou, C. Karamitros, Axarli, I. A., Kotzia, G. A., Karamitros, C., N. E. Labrou, Chronopoulou, E. G., Platis, D., G. A. Kotzia   +11 more
core   +1 more source

Comparative transcriptome analysis reveals key genes associated with meiotic stability and high seed setting rate in tetraploid rice

BMC Plant Biology
Background Polyploid rice has a high yield potential and excellent nutritional quality. The development of polyploid rice remained critically limited for several decades due to low seed setting rate until the successful breeding of polyploid meiosis ...
Pincang Lv, Man Wang, Rongjie Qiu, Chang Yao, Meng Fang, Yuandong Xing, Xianhua Zhang, Yuchi He, Detian Cai, Zhaojian Song   +9 more
doaj   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

Enzyme engineering for industrial applications at BASF [PDF]

, 2019
BASF, a leader in performance chemicals, creates sustainable solutions for agricultural, food and industrial applications by harnessing the power of biotechnology.
Davenport, Adrienne
core   +1 more source

Enzyme Cascade Reaction Engineering

, 2021
Biocatalytic transformations are of growing interest due to their high chemo-, regio-, and enantioselectivity, sustainability, and biocompatibility. In order to build on these advantages, to reinforce them, and not to weaken them, as well as to ensure the fulfillment of economic framework conditions, it is important to harmonize the reaction with its ...
Petermeier, Philipp, Kara, Selin
openaire   +2 more sources

Electron transfer between complexes III and IV in S. cerevisiae mitochondrial membranes

FEBS Letters, EarlyView.
Mitochondrial oxidative phosphorylation in S. cerevisiae mitoplasts is limited by complex IV catalytic capacity, rather than two‐dimensional cytochrome c diffusion. At physiological cytochrome c : supercomplex ratios at salinity equivalent to that of 20 mm monovalent salt, activity is maximized, indicating that this low ionic strength accurately mimics
Ana Paula Lobez, Mikael Oliveberg, Peter Brzezinski   +2 more
wiley   +1 more source

Tests of predictions made by the Equilibrium Model for the effect of temperature on enzyme activity [PDF]

, 2008
The Classical Model describing the effects of temperature on enzyme activity consists of two processes: the catalytic reaction defined by ΔG cat and irreversible inactivation defined by ΔG inact, this model however, does not account for the observed ...
Oudshoorn, Matthew Leslie
core  

Enzyme dynamics and activity: timescale dependence of dynamical transitions in glutamate dehydrogenase solution [PDF]

, 1999
We have examined the temperature dependence of motions in a cryosolution of the enzyme glutamate dehydrogenase (GDH) and compared these with activity.
Ferrand M, Daniel RM, Daniel, Roy, Thomas, Ferrand, Michel, Finney J L, Daniel, Roy,, Finney, John, Dunn R, Reat, Valerie, Reat V, Smith, Jeremy C., Smith, Jeremy, Finney, John L., Dunn, Rachel, Dunn, Rachel V., Smith JC, Daniel, Roy M.   +16 more
core   +1 more source

Engineering enzyme catalysis: an inverse approach [PDF]

Bioscience Reports, 2019
Abstract Enzymes’ inherent chirality confers their exquisite enantiomeric specificity and makes their use as green alternatives to chiral metal complexes or chiral organocatalysts invaluable to the fine chemical industry. The most prevalent way to alter enzyme activity in terms of regioselectivity and stereoselectivity for both industry ...
openaire   +3 more sources