Results 51 to 60 of about 365,104 (302)

Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins

Microbial Cell Factories, 2019
Background The methylotrophic yeast Pichia pastoris is well-known for the production of a broad spectrum of functional types of heterologous proteins including enzymes, antigens, engineered antibody fragments, and next gen protein scaffolds and many ...
Xueyun Zheng, Yimin Zhang, Xinying Zhang, Cheng Li, Xiaoxiao Liu, Ying Lin, Shuli Liang   +6 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Decoupling enzyme catalysis from thermal denaturation [PDF]

, 2010
The equilibrium model (EM) (Daniel et al., 2001) postulates two forms of a folded enzyme, one catalytically active (Eact) and the other inactive (Einact), which interconvert via a fast thermal equilibrium (Keq) (Figure A). This model for enzyme catalysis
Easter, Ashley Davys
core  

Enzyme Microb. Technol. [PDF]

, 2002
The glucose oxidase system was adapted for estimation of the overall oxygen transfer rate in a periodic pressure oscillating, solid-state bioreactor. Enzyme concentration of 40 ml enzyme preparation L-1 was found adequate to give linear gluconic acid ...
Li, ZH, Zhou, XH, Zhao, H, Zhang, XY
core   +2 more sources

A Whole-Process Visible Strategy for the Preparation of Rhizomucor miehei Lipase with Escherichia coli Secretion Expression System and the Immobilization

Microbial Cell Factories
Background Rhizomucor miehei (RM) lipase is a regioselective lipase widely used in food, pharmaceutical and biofuel industries. However, the high cost and low purity of the commercial RM lipase limit its industrial applications.
Mingjun Yang, Xianhui Su, Jun Yang, Zhiwen Lu, Jie Zhou, Fei Wang, Yang Liu, Lixin Ma, Chao Zhai   +8 more
doaj   +1 more source

A new strategy for cellulases application in high temperature industrial scenarios with syringic acid assisting

GCB Bioenergy, 2023
In the process of bioethanol production, more stable and active cellulase in high temperature condition is required. In this study, syringic acid was applied in cellulase hydrolysis system.
Qiuping Ran, Mengtian Huang, Huanan Li, Bixin Chen, Lili He, Xinxin Cui, Jiashu Liu, Zhengbing Jiang   +7 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Computational approach to alter enzyme specificity: case study of D-glucuronate dehydrogenase from Chromohalobacter salexigens [PDF]

, 2010
1
이신엽, 이선복
core  

The alkynyl-containing compounds from mushrooms and their biological activities

Natural Products and Bioprospecting, 2023
Mushrooms have been utilized by humans for thousands of years due to their medicinal and nutritional properties. They are a crucial natural source of bioactive secondary metabolites, and recent advancements have led to the isolation of several alkynyl ...
Ji-shuang Qi, Yingce Duan, Zhao-chen Li, Jin-ming Gao, Jianzhao Qi, Chengwei Liu   +5 more
doaj   +1 more source

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source