SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Process Optimization and Application of Lipase Immobilized on Phenol Amino Modified MCM-41
Shipin gongye ke-jiIn order to construct a new catalytic system of immobilized lipase and improve the stability of enzyme, the phenol amino modified ordered mesoporous material (MCM-41) was introduced for the study of lipase immobilization.
Qian LI +8 more
doaj +1 more source
Improving the Enzymatic Cascade of Reactions for the Reduction of CO2 to CH3OH in Water: From Enzymes Immobilization Strategies to Cofactor Regeneration and Cofactor Suppression [PDF]
, 2022 Carmela Di Spiridione +2 more
openalex +1 more source
SOD activity of immobilized enzyme mimicking complexes [PDF]
, 2003 A binuclear, imidazolato-bridged, possible superoxide dismutase-mimicking complex (Cu(II)-diethylenetriamino-μ-imidazolato-Zn(II)-tris-aminoethylamine-triperchlorate) was prepared and immobilized on silica gel or among the layers of montmorillonite.
Hernádi, Klára +4 more
core
Enzymatic hollow fiber membrane bioreactor for penicilin hydrolysis [PDF]
, 2002 Continuous enzymatic reaction has been proven as an efficient technique for several industrial applications. In this study, a type of hollow fiber membrane bioreactor where penicillin acylase entrapped within membrane pores was applied to continuously ...
Wenten, I.G., Widiasa, I.N.
core +1 more source
Enzymatic degradation of biopolymers in amorphous and molten states: mechanisms and applications
FEBS Open Bio, EarlyView.This review explains how polymer morphology and thermal state shape enzymatic degradation pathways, comparing amorphous and molten biopolymer structures. By integrating structure–reactivity principles with insights from thermodynamics and enzyme engineering, it highlights mechanisms that enable efficient polymer breakdown.
Anđela Pustak, Aleksandra Maršavelski
wiley +1 more source
Biotransformation of halogenated 2′-deoxyribosides by immobilized lactic acid bacteria [PDF]
, 2012 An efficient and green bioprocess is herein reported to obtain halogenated nucleosides by transglycosylation using immobilized lactic acid bacteria (LAB).
Britos, Claudia Noelia +5 more
core +1 more source
FEBS Open Bio, EarlyView.Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold +11 more
wiley +1 more source
Optimization of Enzymatic Gas-Phase Reactions by Increasing the Long-Term Stability of the Catalyst [PDF]
, 2004 Enzymatic gas-phase reactions are usually performed in continuous reactors, and thus very stable and active catalysts are required to perform such transformations on cost-effective levels.
Büchs, Jochen, +4 more
core +2 more sources
Applications of Graphene Quantum Dots in Biomedical Sensors [PDF]
, 2020 Due to the proliferative cancer rates, cardiovascular diseases, neurodegenerative disorders, autoimmune diseases and a plethora of infections across the globe, it is essential to introduce strategies that can rapidly and specifically detect the ultralow ...
Altintas, Zeynep, Mansuriya, Bhargav D.
core +1 more source