Results 191 to 200 of about 4,886,955 (347)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Optimizing enzyme inhibition analysis: precise estimation with a single inhibitor concentration. [PDF]

Nat Commun
Jang HJ, Song YM, Jeon JS, Yun HY, Kim SK, Kim JK.   +5 more
europepmc   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Comparative Evaluation of the Digestive Enzyme Inhibition, Protein, and Starch Components of Ten <i>Macrotyloma uniflorum</i> (Lam.) Verdc. Accessions. [PDF]

Plants (Basel)
Ogunniyi QA, Molokwu AF, Nkumah AO, Adegoke AA, Oyatomi OA, Ogbole OO, Odeku OA, Fettke J, Abberton MT.   +8 more
europepmc   +1 more source

SIRT4 positively regulates autophagy via ULK1, but independently of HDAC6 and OPA1

FEBS Open Bio, EarlyView.
Cells expressing SIRT4 (H161Y), a catalytically inactive mutant of the sirtuin SIRT4, fail to upregulate LC3B‐II and exhibit a reduced autophagic flux under stress conditions. Interestingly, SIRT4(H161Y) promotes phosphorylation of ULK1 at S638 and S758 that are associated with inhibition of autophagy initiation.
Isabell Lehmkuhl, Khawar Amin, Lydia Gabriel, Nils Hampel, Afshin Iram, Julia Hesse, Constanze Wiek, Jasmin Thuy Vy Nguyen, Helmut Hanenberg, Jürgen Scheller, M. Reza Ahmadian, Björn Stork, Doreen M. Floss, Roland P. Piekorz   +13 more
wiley   +1 more source

Elucidating the Disparate Impact of Purification Levels on <i>Lycium barbarum</i> Phenolic Extracts: Chemical Composition and Hypoglycemic Enzyme Inhibition. [PDF]

Food Sci Nutr
Ma Q, Ran L, Lu L, Zhang L, Thakur K, Wei Z, Mi J, Yan Y.   +7 more
europepmc   +1 more source

Evaluation of in vitro toxicity of common phytochemicals included in weight loss supplements using 1H NMR spectroscopy

FEBS Open Bio, EarlyView.
We investigated the toxicity of 12 active compounds commonly found in herbal weight loss supplements (WLS) using human liver and colon cell models. Epigallocatechin‐3‐gallate was the only compound showing significant toxicity. Metabolic profiling revealed protein degradation, disrupted energy and lipid metabolism suggesting that the inclusion of EGCG ...
Emily C. Davies, Garth L. Maker, Ian F. Musgrave, Samantha Lodge   +3 more
wiley   +1 more source

Phenolic profile of different solvent extracts of <i>Reseda alba</i> L. and evaluation of anti-quorum sensing, antioxidant, and enzyme inhibition activities. [PDF]

Front Nutr
Nacer M, Kalla A, Tamfu AN, Boudiba S, Hanini K, Hioun S, Messaoudi M, Atoki AV, Kucukaydin S, Latifa K, Ceylan O, Boudiba L.   +11 more
europepmc   +1 more source

D-3-phosphoglycerate dehydrogenase [PDF]

, 2018
Grant, Gregory A
core   +1 more source

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

FEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani, Tobias Dreyer, Meriem Naim, Rea Lo Dico, Natalia A. Ignatenko, Viktor Magdolen, Dalila Darmoul   +6 more
wiley   +1 more source