Results 91 to 100 of about 5,877,636 (396)

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Immobilization of Isocitrate Dehydrogenase on Mesoporous Silica Foam for Carbon Dioxide Capture [PDF]

E3S Web of Conferences, 2019
Carbon capture can be realized effectively through isocitrate dehydrogenase reaction and the reaction rate was strongly affected by the environmental parameters such as pH and temperature.
Xia Shunxiang, Zhang Laibao, Veony Enjelia   +2 more
doaj   +1 more source

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

Production and characterization of endo-polygalacturonase from Aspergillus niger in solid-state fermentation in double-surface bioreactor

Brazilian Archives of Biology and Technology, 2011
Endo-polygalacturonase (endo-PG) production by Aspergillus niger T0005/007-2 in solid medium with 170 mm of height was evaluated in a cylindrical double surface bioreactor in 96-h experiments.
Diogo Henrique Hendges, Queli Montanari, Eloane Malvessi, Mauricio Moura da Silveira   +3 more
doaj   +1 more source

Influence of Reactive Oxygen Species on the Enzyme Stability and Activity in the Presence of Ionic Liquids

PLoS ONE, 2013
In this paper, we have examined the effect of ammonium and imidazolium based ionic liquids (ILs) on the stability and activity of proteolytic enzyme α-chymotrypsin (CT) in the presence of cold atmospheric pressure plasma jet (APPJ). The present work aims
P. Attri, E. Choi
semanticscholar   +1 more source

Crowding Promotes the Switch from Hairpin to Pseudoknot Conformation in Human Telomerase RNA

, 2011
Formation of a pseudoknot in the conserved RNA core domain in the ribonucleoprotein human telomerase is required for function. In vitro experiments show that the pseudoknot (PK) is in equilibrium with an extended hairpin (HP) structure.
Antal M., Asakura S., Blasco M. A., Bloomfield V. A., Cao S., Cao S., Chen J. L., Cheung M. S., Comolli L. R., D. Thirumalai, Denesyuk N. A., Dhar A., Feng J., Gilley D., Hyeon C., Kilburn D., Kirkpatrick T. R., Leontis N. B., Lin J. C., McGuffee S. R., Montange R. K., Natalia A. Denesyuk, Pincus D. L., Shaw M. R., Theimer C. A., Theimer C. A., Thirumalai D., Walter H., Whitford P. C., Xia T., Zhou H.-X.   +30 more
core   +1 more source

Structural adaptation of cold-active RTX lipase from Pseudomonas sp. strain AMS8 revealed via homology and molecular dynamics simulation approaches [PDF]

, 2013
The psychrophilic enzyme is an interesting subject to study due to its special ability to adapt to extreme temperatures, unlike typical enzymes. Utilizing computer-aided software, the predicted structure and function of the enzyme lipase AMS8 (LipAMS8 ...
Basri, Mahiran, Ganasen, Menega, Mohamad Ali, Mohd Shukuri, Mohd Fuzi, Siti Farhanie, Raja Abdul Rahman, Raja Noor Zaliha, Salleh, Abu Bakar   +5 more
core   +2 more sources

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Considerations when Measuring Biocatalyst Performance

Molecules, 2019
As biocatalysis matures, it becomes increasingly important to establish methods with which to measure biocatalyst performance. Such measurements are important to assess immobilization strategies, different operating modes, and reactor configurations ...
Mafalda Dias Gomes, John M. Woodley
doaj   +1 more source

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source