Results 71 to 80 of about 5,877,636 (396)

Mapping the evolution of mitochondrial complex I through structural variation

FEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin, Tingting Chen, James A. Letts   +2 more
wiley   +1 more source

Stabilisation of Na,K-ATPase structure by the cardiotonic steroid ouabain [PDF]

, 2013
Cardiotonic steroids such as ouabain bind with high affinity to the membrane-bound cation-transporting P-type Na,K-ATPase, leading to complete inhibition of the enzyme.
Abdul-Gader, Andrew J. Miles, B.A. Wallace, Esmann, Esmann, Esmann, Esmann, Fedosova, Kabsch, Kaplan, Klodos, Klose, Lees, Lowry, Mikael Esmann, Miles, Miles, Miles, Miles, Morth, Natalya U. Fedosova, Ogawa, Provencher, Schoner, Shinoda, Skou, Sreerama, Søren V. Hoffmann, Van Stokkum, Whitmore, Yatime   +30 more
core   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Optimization of Enzymatic Gas-Phase Reactions by Increasing the Long-Term Stability of the Catalyst [PDF]

, 2004
Enzymatic gas-phase reactions are usually performed in continuous reactors, and thus very stable and active catalysts are required to perform such transformations on cost-effective levels.
Büchs, Jochen,, Daussmann, Thomas,, Ferloni, Clara,, Heinemann, Matthias,, Hummel, Werner,   +4 more
core   +2 more sources

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

The Role of Surface Exposed Lysine in Conformational Stability and Functional Properties of Lipase from Staphylococcus Family

Molecules, 2020
Surface charge residues have been recognized as one of the stability determinants in protein. In this study, we sought to compare and analyse the stability and conformational dynamics of staphylococcal lipase mutants with surface lysine mutation using ...
Nurul Nadirah Ahmad, Nor Hafizah Ahmad Kamarudin, Adam Thean Chor Leow, Raja Noor Zaliha Raja Abd. Rahman   +3 more
doaj   +1 more source

Stabilization of Enzymes in Silk Films [PDF]

Biomacromolecules, 2009
Material systems are needed that promote stabilization of entrained molecules, such as enzymes or therapeutic proteins, without destroying their activity. We demonstrate that the unique structure of silk fibroin protein, when assembled into the solid state, establishes an environment that is conducive to the stabilization of entrained proteins. Enzymes
Shenzhou, Lu, Xiaoqin, Wang, Qiang, Lu, Xiao, Hu, Neha, Uppal, Fiorenzo G, Omenetto, David L, Kaplan   +6 more
openaire   +2 more sources

Dynamic Stability and Thermodynamic Characterization in an Enzymatic Reaction at the Single Molecule Level

, 2011
In this work we study, at the single molecular level, the thermodynamic and dynamic characteristics of an enzymatic reaction comprising a rate limiting step.
Chimal-Eguia, Chimal-Eguia, Esposito, Ge, Guzman-Vargas, Holmes, Huang, Huang, Huang, Huang, Huang, Huang, Kitano, Kitano, Lesne, Moisés Santillán, Nie, Nie, Páez-Hernández, Páez-Hernández, Qian, Santillán, Santillán, Santillán, Zeron   +24 more
core   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

Support Materials of Organic and Inorganic Origin as Platforms for Horseradish Peroxidase Immobilization: Comparison Study for High Stability and Activity Recovery

Molecules
In the presented study, a variety of hybrid and single nanomaterials of various origins were tested as novel platforms for horseradish peroxidase immobilization.
Muhammad Bilal, Oliwia Degorska, Daria Szada, Agnieszka Rybarczyk, Agata Zdarta, Michal Kaplon, Jakub Zdarta, Teofil Jesionowski   +7 more
doaj   +1 more source