Results 41 to 50 of about 4,721,201 (422)

Formation of Enzyme-Enzyme Heteroconjugates

open access: yesAgricultural and Biological Chemistry, 1984
Enzyme-enzyme heteroconjugates of phosphoglyceromutase, enolase and pyruvate kinase, which sequentially act to produce ATP in the glycolytic pathway, were constructed with a bifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The conjugates of phospho-glyceromutase-enolase, enolase-pyruvate kinase and phosphoglyceromutase-enolase ...
Katsuzumi Okumura   +3 more
openaire   +3 more sources

Non-toxic total nitrogen determination using a low alkaline persulfate digestion

open access: yesMethodsX, 2020
Measuring total nitrogen, nitrate, and nitrite is critical for compliance with water safety standards. Previous methods for measuring total nitrogen were hazardous, time consuming, and expensive.
Jacob L. Studt   +5 more
doaj  

Purification and Characterization of a Novel Alginate Lyase from the Marine Bacterium Bacillus sp. Alg07

open access: yesMarine Drugs, 2018
Alginate oligosaccharides with different bioactivities can be prepared through the specific degradation of alginate by alginate lyases. Therefore, alginate lyases that can be used to degrade alginate under mild conditions have recently attracted public ...
Peng Chen   +4 more
doaj   +1 more source

The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of pathway/genome databases

open access: yesNucleic Acids Res., 2009
The MetaCyc database (MetaCyc.org) is a comprehensive and freely accessible resource for metabolic pathways and enzymes from all domains of life. The pathways in MetaCyc are experimentally determined, small-molecule metabolic pathways and are curated ...
R. Caspi   +17 more
semanticscholar   +1 more source

Effect of a combination of enzymes on the fundamental rheological behavior of bread dough enriched with resistant starch [PDF]

open access: yes, 2016
The effect of three enzymes on the fundamental rheological parameters of bread dough with high content of resistant starch (RS) was studied. The RS was added as an alternative to increase the fiber ingestion while the enzymes, to overcome the gluten ...
Altuna, Luz   +2 more
core   +2 more sources

High copy number and highly stable Escherichia coli–Bacillus subtilis shuttle plasmids based on pWB980

open access: yesMicrobial Cell Factories, 2020
Background pWB980 derived from pUB110 is a promising expression vector in Bacillus for its high copy number and high stability. However, the low transformation rate of recombinant plasmids to the wild cells limited the application of it.
XingYa Zhao   +8 more
doaj   +1 more source

The MetaCyc database of metabolic pathways and enzymes

open access: yesNucleic Acids Res., 2017
MetaCyc (https://MetaCyc.org) is a comprehensive reference database of metabolic pathways and enzymes from all domains of life. It contains more than 2570 pathways derived from >54 000 publications, making it the largest curated collection of metabolic ...
R. Caspi   +12 more
semanticscholar   +1 more source

Direct Single Molecule Imaging of Enhanced Enzyme Diffusion [PDF]

open access: yesPhys. Rev. Lett. 123, 128101 (2019), 2018
Recent experimental results have shown that active enzymes can diffuse faster when they are in the presence of their substrates. Fluorescence correlation spectroscopy (FCS), which relies on analyzing the fluctuations in fluorescence intensity signal to measure the diffusion coefficient of particles, has typically been employed in most of the prior ...
arxiv   +1 more source

Erythrocytes as Carriers of Therapeutic Enzymes. [PDF]

open access: yes, 2020
Therapeutic enzymes are administered for the treatment of a wide variety of diseases. They exert their effects through binding with a high affinity and specificity to disease-causing substrates to catalyze their conversion to a non-noxious product, to ...
Bax, BE
core   +1 more source

Serratiopeptidase: Insights into the therapeutic applications

open access: yesBiotechnology Reports, 2020
Therapeutic applications of enzymes have been widely accepted in clinical practices for decades. Proteolytic enzymes in particular, have been used for the treatment of diseases and disorders.
Swati B. Jadhav   +4 more
doaj  

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