Results 51 to 60 of about 3,038,812 (378)

High copy number and highly stable Escherichia coli–Bacillus subtilis shuttle plasmids based on pWB980

open access: yesMicrobial Cell Factories, 2020
Background pWB980 derived from pUB110 is a promising expression vector in Bacillus for its high copy number and high stability. However, the low transformation rate of recombinant plasmids to the wild cells limited the application of it.
XingYa Zhao   +8 more
doaj   +1 more source

The protein cost of metabolic fluxes: prediction from enzymatic rate laws and cost minimization [PDF]

open access: yes, 2016
Bacterial growth depends crucially on metabolic fluxes, which are limited by the cell's capacity to maintain metabolic enzymes. The necessary enzyme amount per unit flux is a major determinant of metabolic strategies both in evolution and bioengineering.
arxiv   +1 more source

Enzyme-enriched condensates show self-propulsion, positioning, and coexistence [PDF]

open access: yes, 2023
Enzyme-enriched condensates can organize the spatial distribution of their substrates by catalyzing non-equilibrium reactions. Conversely, an inhomogeneous substrate distribution induces enzyme fluxes through substrate-enzyme interactions. We find that condensates move towards the center of a confining domain when this feedback is weak.
arxiv   +1 more source

Effect of a combination of enzymes on the fundamental rheological behavior of bread dough enriched with resistant starch [PDF]

open access: yes, 2016
The effect of three enzymes on the fundamental rheological parameters of bread dough with high content of resistant starch (RS) was studied. The RS was added as an alternative to increase the fiber ingestion while the enzymes, to overcome the gluten ...
Altuna, Luz   +2 more
core   +2 more sources

Insights into PI3K/AKT signaling in B cell development and chronic lymphocytic leukemia

open access: yesFEBS Letters, EarlyView.
This Review explores how the phosphoinositide 3‐kinase and protein kinase B pathway shapes B cell development and drives chronic lymphocytic leukemia, a common blood cancer. It examines how signaling levels affect disease progression, addresses treatment challenges, and introduces novel experimental strategies to improve therapies and patient outcomes.
Maike Buchner
wiley   +1 more source

Biophysical characterization of the structural stability of Helix lucorum hemocyanin

open access: yesBiotechnology & Biotechnological Equipment, 2021
The structural stability of the hеmocyanin purified from the hеmolymph of gаrden snаils Helix lucorum (HlH) was investigated by means of far-UV circular dichroism (CD), differential scanning calorimetry (DSC) and transmission electron microscopy (TEM ...
Krassimira Idakieva   +5 more
doaj   +1 more source

Insolubilization process increases enzyme stability [PDF]

open access: yes, 1971
Enzymes complexed with polymeric matrices contain properties suggesting application to enzyme-controlled reactions. Stability of insolubilized enzyme derivatives is markedly greater than that of soluble enzymes and physical form of insolubilized enzymes ...
Billingham, J., Lyn, J.
core   +1 more source

Distribution of chromaffin secretory vesicles, acetylcholinesterase, and lysosomal enzymes in sucrose and percoll gradients [PDF]

open access: yes, 1984
Crude chromaffin secretory vesicles, obtained by differential centrifugation, were further purified on isotonic (Percoll) gradients. The chromaffin vesicle fractions recovered from the gradients contain acetylcholinesterase as well as lysosomal enzymes ...
Bowers   +31 more
core   +1 more source

Taurine promotes glucagon‐like peptide‐1 secretion in enteroendocrine L cells

open access: yesFEBS Letters, EarlyView.
Taurine, a sulfur‐containing amino acid, is likely taken up by enteroendocrine L cells via the taurine transporter. This process increases the levels of cytosolic ATP. The increase in intracellular Ca2+ concentrations and glucagon‐like peptide‐1 secretion through membrane depolarization is caused by the closure of ATP‐sensitive potassium channels ...
Yuri Osuga   +6 more
wiley   +1 more source

Determination of Volatile Organic Compounds and Antibacterial Activity of the Amazonian Cyanobacterium Synechococcus sp. Strain GFB01

open access: yesMolecules, 2020
Cyanobacteria exhibit great biotechnological potential due to their capacity to produce compounds with various applicability. Volatile organic compounds (VOCs) possess low molecular weight and high vapor pressure.
Samuel Cavalcante do Amaral   +4 more
doaj   +1 more source

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