Results 81 to 90 of about 3,216,786 (362)

N-Terminal Sequences of Signal Peptides Assuming Critical Roles in Expression of Heterologous Proteins in Bacillus subtilis

open access: yesMicroorganisms
The N-terminal sequences of proteins and their corresponding encoding sequences may play crucial roles in the heterologous expression. In this study, the secretory expression of alkaline pectin lyase APL in B.
Meijuan Zhang   +8 more
doaj   +1 more source

Novel Archaeal DNA Polymerase B from Domas Hot Spring West Java [PDF]

open access: yes, 2015
Nine novel archaeal DNA polymerase genes from Domas Hot Spring, West Java have been cloned directly through the natural sample.
Akhmaloka, A.   +4 more
core  

The relation between visceral adipose tissue accumulation and biochemical tests in university students. [PDF]

open access: yes, 2005
We evaluated the visceral adipose tissue accumulation in university students in Okayama prefecture, Japan. Fifty-eight Japanese university students (10 men and 48 women, age 18.4 +/- 0.6 years)were enrolled in this study.
Fujii, Masafumi   +5 more
core   +1 more source

ENZYMES AND ANTI-ENZYMES OF INFLAMMATORY EXUDATES [PDF]

open access: yesJournal of Experimental Medicine, 1904
The serum of an inflammatory exudate has the power of inhibiting the action of proteolytic ferments contained in the leucocytes. This anti-enzymotic power is possessed by the blood serum from which it doubtless passes into the exudate. In the later stages of inflammation there is some diminution of this anti-enzymotic action.
openaire   +2 more sources

Enzyme-Enzyme Interactions in Monolignol Biosynthesis [PDF]

open access: yesFrontiers in Plant Science, 2019
The enzymes that comprise the monolignol biosynthetic pathway have been studied intensively for more than half a century. A major interest has been the role of pathway in the biosynthesis of lignin and the role of lignin in the formation of wood. The pathway has been typically conceived as linear steps that convert phenylalanine into three major ...
Jack P. Wang   +7 more
openaire   +3 more sources

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

open access: yesFEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue   +6 more
wiley   +1 more source

BsuMI regulates DNA transformation in Bacillus subtilis besides the defense system and the constructed strain with BsuMI-absence is applicable as a universal transformation platform for wild-type Bacillus

open access: yesMicrobial Cell Factories
Background To effectively introduce plasmids into Bacillus species and conduct genetic manipulations in Bacillus chassis strains, it is essential to optimize transformation methods.
Zhao Xingya   +6 more
doaj   +1 more source

The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells

open access: yesFEBS Letters, EarlyView.
The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman   +3 more
wiley   +1 more source

Targeting EZH2 reverses thyroid cell dedifferentiation and enhances iodide uptake in anaplastic thyroid cancer

open access: yesFEBS Letters, EarlyView.
Anaplastic thyroid cancer (ATC) lacks iodide uptake ability due to MAPK activation increasing the expression of the histone methyltransferase EZH2, which represses thyroid differentiation genes (TDGs) such as the sodium iodide symporter (NIS). Dual inhibition of MAPK (U0126) and EZH2 (EPZ6438/Tazemetostat) reverses this mechanism, thus restoring TDG ...
Diego Claro de Mello   +6 more
wiley   +1 more source

Influence of enzymatic cow milk hydrolysates on IgA and IgG response of Balb/c mice organism

open access: yesCzech Journal of Food Sciences, 2005
The manuscript presents the application of an animal model, Balb/c mice, in studies aimed at identifying among enzymatic hydrolysates of sodium caseinate and whey proteins the product with the most beneficial impact on the animal organism.
Barbara Wróblewska   +1 more
doaj   +1 more source

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