Results 1 to 10 of about 1,790,692 (215)

Long-Term In Vitro Expansion of Epithelial Stem Cells Enabled by Pharmacological Inhibition of PAK1-ROCK-Myosin II and TGF-β Signaling [PDF]

Cell Reports, 2018
Summary: Despite substantial self-renewal capability in vivo, epithelial stem and progenitor cells located in various tissues expand for a few passages in vitro in feeder-free condition before they succumb to growth arrest.
Chengkang Zhang, Hyung Joo Lee, Anura Shrivastava, Ruipeng Wang, Travis J. McQuiston, Sharon S. Challberg, Brian A. Pollok, Ting Wang   +7 more
doaj   +3 more sources

Modeling Gastrointestinal Diseases Using Organoids to Understand Healing and Regenerative Processes

Cells, 2021
The gastrointestinal tract is a continuous series of organs from the mouth to the esophagus, stomach, intestine and anus that allows digestion to occur.
Alexane Ollivier, Maxime M. Mahe, Géraldine Guasch   +2 more
doaj   +1 more source

Epithelial cells removed in advanced surface ablation (ASA) surgery can be used as a source of corneal samples to perform in vitro studies [PDF]

, 2022
Producción CientíficaHuman corneal epithelial cells are needed to study corneal pathophysiology in vitro. Due to the limitations of cell lines, the use of primary cells is highly desirable, but the scarcity of human tissues, along with ethical issues ...
Diebold Luque, María Yolanda, García Posadas, Laura, Maldonado López, Miguel José   +2 more
core   +1 more source

A stem cell population at the anorectal junction maintains homeostasis and participates in tissue regeneration

Nature Communications, 2021
Transition zones connect distinct epithelia, contain cells expressing stem cell markers, and contribute to cancer development. Here, the authors examine the mouse anorectal junction, identifying a population of Krt17-positive basal cells that contribute ...
Louciné Mitoyan, Véronique Chevrier, Hector Hernandez-Vargas, Alexane Ollivier, Zeinab Homayed, Julie Pannequin, Flora Poizat, Cécile De Biasi-Cador, Emmanuelle Charafe-Jauffret, Christophe Ginestier, Géraldine Guasch   +10 more
doaj   +1 more source

BMI1 nuclear location is critical for RAD51-dependent response to replication stress and drives chemoresistance in breast cancer stem cells

Cell Death and Disease, 2022
Replication stress (RS) has a pivotal role in tumor initiation, progression, or therapeutic resistance. In this study, we depicted the mechanism of breast cancer stem cells’ (bCSCs) response to RS and its clinical implication.
Violette Azzoni, Julien Wicinski, Manon Macario, Martin Castagné, Pascal Finetti, Katerina Ambrosova, Célia D. Rouault, Arnaud Sergé, Anne Farina, Emilie Agavnian, Sergiu Coslet, Emmanuelle Josselin, Arnaud Guille, José Adelaide, Emmanouil Zacharioudakis, Rémy Castellano, Francois Bertucci, Daniel Birnbaum, Raphael Rodriguez, Emmanuelle Charafe-Jauffret, Christophe Ginestier   +20 more
doaj   +1 more source

From Submerged Cultures to 3D Cell Culture Models: Evolution of Nasal Epithelial Cells in Asthma Research and Virus Infection

Viruses, 2021
Understanding the response to viral infection in the context of respiratory diseases is of significant importance. Recently, there has been more focus on the role of the nasal epithelium in disease modeling.
Malik Aydin, Ella A. Naumova, Aliyah Bellm, Ann-Kathrin Behrendt, Federica Giachero, Nora Bahlmann, Wenli Zhang, Stefan Wirth, Friedrich Paulsen, Wolfgang H. Arnold, Anja Ehrhardt   +10 more
doaj   +1 more source

Experimental and spontaneous metastasis assays can result in divergence in clonal architecture

Communications Biology, 2023
Intratumoural heterogeneity is associated with poor outcomes in breast cancer. To understand how malignant clones survive and grow in metastatic niches, in vivo models using cell lines and patient-derived xenografts (PDX) have become the gold standard ...
Antonin Serrano, Tom Weber, Jean Berthelet, Farrah El-Saafin, Sreeja Gadipally, Emmanuelle Charafe-Jauffret, Christophe Ginestier, John M. Mariadason, Samantha R. Oakes, Kara Britt, Shalin H. Naik, Delphine Merino   +11 more
doaj   +1 more source

Klebsiella pneumoniae is able to trigger epithelial-mesenchymal transition process in cultured airway epithelial cells [PDF]

, 2016
The ability of some bacterial pathogens to activate Epithelial-Mesenchymal Transition normally is a consequence of the persistence of a local chronic inflammatory response or depends on a direct interaction of the pathogens with the host epithelial cells.
A García-Fernández, A Markogiannakis, A Moustakas, AA Franco, BC Willis, C Passariello, D Kovacs, D Ranieri, Daniela Martinelli, E Candelario-Jalil, Francesca Mazzetta, G Cane, HY Kim, I Pilpilidis, I Santino, Iolanda Santino, J Martinez, JAB Strickertsson, JC Lin, JI Bàrdos, José A. Bengoechea, K Nishi, KK Kim, L Leone, LA Borthwick, Laura Leone, M Barbara, Maurizio Alimandi, ME Falagas, MH Yang, MS Wright, NS Chandel, P Chandrakesan, P Hofman, P Nordmann, R Kalluri, S De Majumdar, S Lamouille, Sabatino Valente, Salvatore Raffa, SS Leonard, SZ Ding, V Cano, Y Kondo, Y Saito, Y Yin, YT Oh   +46 more
core   +6 more sources

A mammalian Wnt5a-Ror2-Vangl2 axis controls the cytoskeleton and confers cellular properties required for alveologenesis. [PDF]

, 2020
Alveolar formation increases the surface area for gas-exchange and is key to the physiological function of the lung. Alveolar epithelial cells, myofibroblasts and endothelial cells undergo coordinated morphogenesis to generate epithelial folds (secondary
Chou, Yu-Ting, Chuang, Pao-Tien, Li, Jianying, Lin, Chuwen, Wolters, Paul J, Wong, Julia, Yao, Erica, Zhang, Kuan   +7 more
core   +1 more source

Normal epithelial and triple-negative breast cancer cells show the same invasion potential in rigid spatial confinement [PDF]

New J. Phys. 21 (2019) 083016, 2020
The extra-cellular microenvironment has a fundamental role in tumor growth and progression, strongly affecting the migration strategies adopted by single cancer cells during metastatic invasion. In this study, we use a novel microfluidic device to investigate the ability of mesenchymal and epithelial breast tumor cells to fluidize and migrate through ...
arxiv   +1 more source