Results 251 to 260 of about 96,540 (301)
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1998
Epitope mapping can be used to identify areas of a protein that an antibody recognizes and binds to. Monoclonal antibodies are easier to characterize, but epitope maps can also be produced for polyclonal antisera.
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Epitope mapping can be used to identify areas of a protein that an antibody recognizes and binds to. Monoclonal antibodies are easier to characterize, but epitope maps can also be produced for polyclonal antisera.
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Mapping conformational epitopes by NMR spectroscopy
Current Opinion in Virology, 2021Antibodies recognize their target with high affinity and specificity. This is important for pathogen neutralization, which plays a crucial role in defense against disease. Antibodies are powerful tools in the development of new therapeutics, such as vaccines, to fight diseases such as viral infections and even cancer.
Ana P, Valente +1 more
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Molecular Biology Reports, 1992
It is important to remember that the merits of the different approaches to epitope mapping should be judged against the purpose of the study. A peptide specifically recognized by nearly all sera containing a certain autoimmune specificity [20, 21, 26], would most likely be selected for the detection of the anti-linear/continuous epitope fraction among ...
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It is important to remember that the merits of the different approaches to epitope mapping should be judged against the purpose of the study. A peptide specifically recognized by nearly all sera containing a certain autoimmune specificity [20, 21, 26], would most likely be selected for the detection of the anti-linear/continuous epitope fraction among ...
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Proteolytic Fragmentation for Epitope Mapping
2003The use of antigen fragments generated by specific proteolytic cleavage is a relatively simple "library" approach for epitope mapping in which possible overlapping fragments are screened with the antibody on Western blots. Proteolytic fragmentation with numerous proteases having different cleavage specificites can be carried out on native and ...
MAZZONI, MARIA ROSA +2 more
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Epitope mapping of human thyrotropin
Acta Endocrinologica, 1989Abstract. Epitope mapping of hTSH was carried out using 19 monoclonal antibodies prepared with hTSH or its β-subunit as antigen. The affinity constants of the monoclonal antibodies ranged from 9.6 × 107 to 5.7 × 109 mol/l for hTSH. The binding activities of monoclonal antibodies were maintained or in some cases rather enhanced after removal of the ...
Y, Endo +6 more
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2009
Despite its unequivocal benefits to humankind, vaccine design and development has always been an inherently laborious and a largely empirical process; the unfortunate lack of a rational basis for vaccinology has hitherto stymied the commercial exploitation of vaccine discovery and also the deployment of vaccination as the principal, global instrument ...
Matthew N. Davies, Darren R. Flower
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Despite its unequivocal benefits to humankind, vaccine design and development has always been an inherently laborious and a largely empirical process; the unfortunate lack of a rational basis for vaccinology has hitherto stymied the commercial exploitation of vaccine discovery and also the deployment of vaccination as the principal, global instrument ...
Matthew N. Davies, Darren R. Flower
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Intact Transition Epitope Mapping (ITEM)
Journal of the American Society for Mass Spectrometry, 2017Intact transition epitope mapping (ITEM) enables rapid and accurate determination of protein antigen-derived epitopes by either epitope extraction or epitope excision. Upon formation of the antigen peptide-containing immune complex in solution, the entire mixture is electrosprayed to translate all constituents as protonated ions into the gas phase ...
Yelena, Yefremova +4 more
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An Introduction to Epitope Mapping
2018Antibodies are protein molecules used routinely for therapeutic, diagnostic, and research purposes due to their exquisite ability to selectively recognize and bind a given antigen. The particular area of the antigen recognized by the antibody is called the epitope, and for proteinaceous antigens the epitope can be of complex nature.
Johan, Nilvebrant, Johan, Rockberg
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2001
Abstract Epitope Mapping covers all the major methods for the identification and definition of epitopes. The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. It can be adapted for the delineation of both helper T cells and cytotoxic T cells.
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Abstract Epitope Mapping covers all the major methods for the identification and definition of epitopes. The Pepscan assay is used to define B cell epitopes and makes use of synthetic peptides but can only be used if the amino acid sequence is known. It can be adapted for the delineation of both helper T cells and cytotoxic T cells.
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2003
The majority of T cells recognize peptide epitopes bound to major histocompatibility complex (MHC)-encoded glycoproteins on the surface of professional antigen-presenting cells (APC), principally dendritic cells, macrophages, and B cells (1-3). Most T cells are specific for peptide epitopes in association with either classical MHC class Ia molecules ...
A A, Delvig, J H, Robinson
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The majority of T cells recognize peptide epitopes bound to major histocompatibility complex (MHC)-encoded glycoproteins on the surface of professional antigen-presenting cells (APC), principally dendritic cells, macrophages, and B cells (1-3). Most T cells are specific for peptide epitopes in association with either classical MHC class Ia molecules ...
A A, Delvig, J H, Robinson
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