Structural analysis of the adenovirus type 2 E3/19K protein using mutagenesis and a panel of conformation-sensitive monoclonal antibodies [PDF]
, 2008 The E3/19K protein of human adenovirus type 2 (Ad2) was the first viral protein shown to interfere with antigen presentation. This 25 kDa transmembrane glycoprotein binds to major histocompatibility complex (MHC) class I molecules in the endoplasmic ...
Ahmed +62 more
core +1 more source
Immune epitope database analysis resource [PDF]
, 2012 The immune epitope database analysis resource (IEDB-AR: http://tools.iedb.org) is a collection of tools for prediction and analysis of molecular targets of T- and B-cell immune responses (i.e. epitopes).
A. Sette +33 more
core +1 more source
Determination of a predictive cleavage motif for eluted major histocompatibility complex class II ligands [PDF]
, 2018 CD4+ T cells have a major role in regulating immune responses. They are activated by recognition of peptides mostly generated from exogenous antigens through the major histocompatibility complex (MHC) class II pathway.
Dhanda, Sandeep Kumar +7 more
core +6 more sources
Predicting the effectiveness of hepatitis C virus neutralizing antibodies by bioinformatic analysis of conserved epitope residues using public sequence data [PDF]
, 2018 Hepatitis C virus (HCV) is a global health issue. Although direct-acting antivirals are available to target HCV, there is currently no vaccine. The diversity of the virus is a major obstacle to HCV vaccine development. One approach toward a vaccine is to
Cowton, Vanessa M. +3 more
core +2 more sources
Serodominant SARS-CoV-2 Nucleocapsid Peptides Map to Unstructured Protein Regions
Microbiology Spectrum, 2023 The SARS-CoV-2 nucleocapsid (N) protein is highly immunogenic, and anti-N antibodies are commonly used as markers for prior infection. While several studies have examined or predicted the antigenic regions of N, these have lacked consensus and structural
Jacob P. Vandervaart +10 more
doaj +1 more source
Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq. [PDF]
, 2016 BackgroundThe robustness of ChIP-seq datasets is highly dependent upon the antibodies used. Currently, polyclonal antibodies are the standard despite several limitations: They are non-renewable, vary in performance between lots and need to be validated ...
Busby, Michele +11 more
core +1 more source
Identification of hepatitis a virus mimotopes by phage display, antigenicity and immunogenicity [PDF]
, 2007 A phage-displayed peptide approach was used to identify ligands mimicking antigenic determinants of hepatitis A virus (HAV) for the first time. Bacteriophages displaying HAV mimotopes were isolated from a phage-display peptide library by affinity ...
Alicia Aguilar +54 more
core +1 more source
Acta Chimica Slovenica, 2016 Detailed knowledge of antigenic determinants is crucial when characterizing therapeutic and diagnostic antibodies, assessing vaccine effectiveness and developing epitope-based vaccines. Most epitope mapping approaches are labor intensive and costly.
Tomaž Bratkovič, Peter Molek
doaj +1 more source
Comparative characteristic of the methods of protein antigens epitope mapping [PDF]
The Ukrainian Biochemical Journal, 2014 Comparative analysis of experimental methods of epitope mapping of protein antigens has been carried out. The vast majority of known techniques are involved in immunochemical study of the interaction of protein molecules or peptides with antibodies of ...
O. Yu. Galkin
doaj +1 more source
eLife, 2021 Mapping polyclonal serum responses is critical to rational vaccine design. However, most high-resolution mapping approaches involve isolating and characterizing individual antibodies, which incompletely defines the polyclonal response.
Adam S Dingens +10 more
doaj +1 more source