Epitope Mapping of Polyclonal Antibodies by Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS).
Analytical Chemistry, 2021 Epitope mapping of antibodies (Abs) is crucial for understanding adaptive immunity, as well as studying the mode of action of therapeutic antibodies and vaccines.
Susanne Ständer +4 more
semanticscholar +1 more source
Identification of a blockade epitope of human norovirus GII.17
Emerging Microbes and Infections, 2021 Human noroviruses are the dominant causative agent of acute viral gastroenteritis worldwide. During the winter of 2014–2015, genotype GII.17 cluster IIIb strains emerged as the leading cause of norovirus infection in Asia and later spread to other parts ...
Yufang Yi +7 more
doaj +1 more source
Identification of immunodominant CD4-restricted epitopes co-located with antibody binding sites in individuals vaccinated with ALVAC-HIV and AIDSVAX B/E. [PDF]
PLoS ONE, 2015 We performed fine epitope mapping of the CD4+ responses in the ALVAC-HIV-AIDSVAX B/E prime-boost regimen in the Thai Phase III trial (RV144). Non-transformed Env-specific T cell lines established from RV144 vaccinees were used to determine the fine ...
Silvia Ratto-Kim +17 more
doaj +1 more source
High-Throughput B Cell Epitope Determination by Next-Generation Sequencing
Frontiers in Immunology, 2022 Development of novel technologies for the discovery of human monoclonal antibodies has proven invaluable in the fight against infectious diseases. Among the diverse antibody repertoires elicited by infection or vaccination, often only rare antibodies ...
Lauren M. Walker +27 more
doaj +1 more source
Epitope Mapping with Diethylpyrocarbonate Covalent Labeling-Mass Spectrometry.
Analytical Chemistry, 2021 Antigen-antibody epitope mapping is essential for understanding binding mechanisms and developing new protein therapeutics. In this study, we investigate diethylpyrocarbonate (DEPC) covalent labeling-mass spectrometry as a means of analyzing antigen ...
Catherine Y Tremblay +2 more
semanticscholar +1 more source
TITAN: T Cell Receptor Specificity Prediction with Bimodal Attention Networks [PDF]
Bioinformatics 37 (2021): i237-i244, 2021 Motivation: The activity of the adaptive immune system is governed by T-cells and their specific T-cell receptors (TCR), which selectively recognize foreign antigens. Recent advances in experimental techniques have enabled sequencing of TCRs and their antigenic targets (epitopes), allowing to research the missing link between TCR sequence and epitope ...
arxiv +1 more source
Epitope mapping using mRNA display and a unidirectional nested deletion library [PDF]
, 2005 In vitro selection targeting an anti-polyhistidine monoclonal antibody was performed using mRNA display with a random, unconstrained 27-mer peptide library.
Ja, William W. +2 more
core +1 more source
Inadequate Reference Datasets Biased toward Short Non-epitopes Confound B-cell Epitope Prediction [PDF]
, 2016 X-ray crystallography has shown that an antibody paratope typically binds 15-22 amino acids (aa) of an epitope, of which 2-5 randomly distributed amino acids contribute most of the binding energy.
Chowdhury, Erfan U. +3 more
core +1 more source
BeeTLe: A Framework for Linear B-Cell Epitope Prediction and Classification [PDF]
, 2023 The process of identifying and characterizing B-cell epitopes, which are the portions of antigens recognized by antibodies, is important for our understanding of the immune system, and for many applications including vaccine development, therapeutics, and diagnostics.
arxiv +1 more source
High Throughput T Epitope Mapping and Vaccine Development [PDF]
, 2010 Mapping of antigenic peptide sequences from proteins of relevant pathogens recognized by T helper (Th) and by cytolytic T lymphocytes (CTL) is crucial for vaccine development. In fact, mapping of T-cell epitopes provides useful information for the design
Ivaldi, Federico +3 more
core +2 more sources