Results 61 to 70 of about 1,047,095 (303)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

EFFECT OF COOLING RATE AND GRAIN REFINEMENT ON THE MICROSEGREGATION IN Al-4.8 wt.% Cu ALLOY

open access: yesIranian Journal of Materials Science and Engineering, 2015
Microsegregation is one of the most important phenomena occurs during solidification. It usually results in formation of some unexpected second phases which generally affect the mechanical properties and specially reduce the workability of casting ...
M. Dehnavi   +3 more
doaj  

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

An improved slip boundary condition for wall-modeled large-eddy simulation of flow separation using high-order finite-difference method [PDF]

open access: yesAIP Advances
Simulating flow separation in turbulent flows at high Reynolds numbers remains a significant challenge for high-order finite-difference methods. This study introduces a non-equilibrium slip boundary condition, integrated into a wall-modeled large-eddy ...
高磊   +5 more
doaj   +1 more source

A Case Study in Partial Specification: Consistency and Refinement for Object-Z [PDF]

open access: yes, 2000
The 'viewpoint' approach, in which a system is described by several partial specifications, has been proposed as a way of making complex computing systems more understandable.
Derrick, J., Taylor, C., Boiten, E.
core   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

open access: yesFEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay   +2 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Mode refinement algorithm for H.264 inter frame requantization [PDF]

open access: yes, 2006
The latest video coding standard H.264 has been recently approved and has already been adopted for numerous applications including HD-DVD and satellite broadcast.
Damien Lefol   +3 more
core   +1 more source

Heterotropic regulation and negative homotropic cooperativity

open access: yesFEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea   +5 more
wiley   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

open access: yesFEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey   +4 more
wiley   +1 more source

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