Cloning and expression in Escherichia coli of pectinase genes of Erwinia carotovora subsp. carotovora [PDF]
Genes coding for an endo-pectate lyase, an exo-pectate lyase, and an endopolygalacturonase of Erwinia carotovora subsp. carotovora Ecc71 were cloned in Escherichia coli HB101, using the cosmid pHC79. The products of the cloned pectinase genes paralleled their counterparts in strain Ecc71 in isoelectric mobility, mode of substrate degradation, and ...
R T, Zink, A K, Chatterjee
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Seven new genes controlled by the quorum-sensing signal molecule N-(3-oxohexanoyl)-L-homoserine lactone (OHHL) have been identified in Erwinia carotovora subsp. carotovora.
C. L. Pemberton +11 more
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Genotypic characterisation of the Erwinia genus by PCR-RFLP analysis of rpoS gene
Genotypic characterisation of the members of the genus Erwinia, based on the PCR-RFLP analysis of a fragment of the rpoS gene was done. PCR primers deduced from described rpoS gene sequences of E.
M. Waleron, K. Waleron, E. Łojkowska
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The objetive of this work was to identify the pectolytic bacteria associated with soft rot of arracacha roots in Brazil. From 1998 to 2001, 227 isolates of Erwinia spp.
Gilmar Paulo Henz +2 more
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Identification of Arabidopsis thaliana genes responsive to plant cell-wall-degrading enzymes of Erwinia carotovora subsp. carotovora led to the isolation of a cDNA clone with high sequence homology to the gene for allene oxide synthase, an enzyme ...
Cecilia Norman +2 more
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Caracterização de bactérias do género Erwinia causando podridões em plantas ornamentais no Brasil.
São descritas as ocorrências de fito-bacterioses em plantios comerciais, ocasionando podridão mole em seis espécies de plantas ornamentais. Testes bioquímicos, fisiológicos, culturais e de patogenicidade permitiram cons-tatar a ocorrência de Erwinia ...
Irene M.G. Almeida +2 more
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Characterization of transposon insertion out- mutants of Erwinia carotovora subsp. carotovora defective in enzyme export and of a DNA segment that complements out mutations in E. carotovora subsp. carotovora, E. carotovora subsp. atroseptica, and Erwinia chrysanthemi [PDF]
Soft-rotting Erwinia spp. export degradative enzymes to the cell exterior (Out+), a process contributing to their ability to macerate plant tissues. Transposon (Tn5, Tn10, Tn10-lacZ) insertion Out- mutants were obtained in Erwinia carotovora subsp. carotovora 71 by using plasmid and bacteriophage lambda delivery systems.
H, Murata +4 more
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Quorum Sensing in the Plant Pathogen Erwinia carotovora subsp. carotovora: The Role of expREcc
The production of the main virulence determinants of the plant pathogen Erwinia carotovora subsp. carotovora, the extracellular cell wall-degrading enzymes, is partly controlled by the diffusible signal molecule N-(3-oxohexanoyl)-l-homoserine lactone ...
Robert A. Andersson +8 more
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Chromosomal mapping in Erwinia carotovora subsp. carotovora with the IncP plasmid R68::Mu [PDF]
Conjugational gene transfer was established in Erwinia carotovora subsp. carotovora SCRI193 by using plasmid R68::Mu c+ to mobilize the chromosome into multiply mutant recipients. It was observed that although the plasmid alone mobilized markers randomly at a frequency of ca.
K J, Forbes, M C, Pérombelon
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Erwinia carotovora subsp. carotovora is a causal agent of soft-rot diseases in a wide variety of plants. Here, we have isolated a new regulatory factor involved in the virulence of E. carotovora subsp.
Hiroyuki Matsumoto +4 more
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