Results 11 to 20 of about 1,495,661 (364)

Transforming Shiga toxin-producing Escherichia coli surveillance through whole genome sequencing in food safety practices

Frontiers in Microbiology, 2023
IntroductionShiga toxin-producing Escherichia coli (STEC) is a gastrointestinal pathogen causing foodborne outbreaks. Whole Genome Sequencing (WGS) in STEC surveillance holds promise in outbreak prevention and confinement, in broadening STEC epidemiology
Stéphanie Nouws, Stéphanie Nouws, Bavo Verhaegen, Sarah Denayer, Florence Crombé, Denis Piérard, Bert Bogaerts, Kevin Vanneste, Kathleen Marchal, Kathleen Marchal, Nancy H. C. Roosens, Sigrid C. J. De Keersmaecker   +11 more
doaj   +1 more source

High Prevalence of ST131 Subclades C2-H30Rx and C1-M27 Among Extended-Spectrum β-Lactamase-Producing Escherichia coli Causing Human Extraintestinal Infections in Patients From Two Hospitals of Spain and France During 2015

Frontiers in Cellular and Infection Microbiology, 2020
The present study was carried out to evaluate the prevalence of sequence type 131 (ST131) among 188 extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) collected in 2015 in Lucus Augusti University hospital (Lugo, Spain) and AP-HP Beaujon ...
Saskia-Camille Flament-Simon, Saskia-Camille Flament-Simon, Vanesa García, Vanesa García, Marion Duprilot, Marion Duprilot, Noémie Mayer, María Pilar Alonso, María Pilar Alonso, Isidro García-Meniño, Isidro García-Meniño, Jesús E. Blanco, Jesús E. Blanco, Miguel Blanco, Miguel Blanco, Marie-Hélène Nicolas-Chanoine, Marie-Hélène Nicolas-Chanoine, Jorge Blanco, Jorge Blanco   +18 more
doaj   +1 more source

Application and Perspectives of MALDI–TOF Mass Spectrometry in Clinical Microbiology Laboratories

Microorganisms, 2021
Early diagnosis of severe infections requires of a rapid and reliable diagnosis to initiate appropriate treatment, while avoiding unnecessary antimicrobial use and reducing associated morbidities and healthcare costs.
Eva Torres-Sangiao, Cristina Leal Rodriguez, Carlos García-Riestra   +2 more
doaj   +1 more source

Surface proteins of Shiga toxin-producing Escherichia coli mediate association with milk fat globules in raw milk

Frontiers in Microbiology, 2023
IntroductionBy adhering to host cells and colonizing tissues, bacterial pathogens can successfully establish infection. Adhesion is considered the first step of the infection process and bacterial adhesion to anti-adhesive compounds is now seen as a ...
Arthur Bagel, Marion Bouvier-Crozier, Marion Bouvier-Crozier, Mélissa Canizares, Badis Hamadou, Louise Courcol, Christelle Lopez, Valérie Michel, Thomas Douellou, Delphine Sergentet, Delphine Sergentet   +10 more
doaj   +1 more source

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

Proceedings of the National Academy of Sciences of the United States of America, 2000
We have developed a simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s).
K. Datsenko, B. Wanner
semanticscholar   +1 more source

Escherichia coli Biofilms [PDF]

, 2008
Escherichia coli is a predominant species among facultative anaerobic bacteria of the gastrointestinal tract. Both its frequent community lifestyle and the availability of a wide array of genetic tools contributed to establish E. coli as a relevant model organism for the study of surface colonization.
Beloin, Christophe, Roux, Agnès, Ghigo, Jean-Marc   +2 more
openaire   +5 more sources

Genomic Characterization of Prevalent mcr-1, mcr-4, and mcr-5 Escherichia coli Within Swine Enteric Colibacillosis in Spain

Frontiers in Microbiology, 2019
Antimicrobial agents are crucial for the treatment of many bacterial diseases in pigs, however, the massive use of critically important antibiotics such as colistin, fluoroquinolones and 3rd–4th-generation cephalosporins often selects for co-resistance ...
Isidro García-Meniño, Dafne Díaz-Jiménez, Vanesa García, María de Toro, Saskia C. Flament-Simon, Jorge Blanco, Azucena Mora   +6 more
doaj   +1 more source

Comprehensive Statistical Evaluation of Etest^®, UMIC^®, MicroScan and Disc Diffusion versus Standard Broth Microdilution: Workflow for an Accurate Detection of Colistin-Resistant and Mcr-Positive E. coli

Antibiotics, 2020
Four colistin susceptibility testing methods were compared with the standard broth microdilution (BMD) in a collection of 75 colistin-susceptible and 75 mcr-positive E. coli, including ST131 isolates.
Isidro García-Meniño, Pilar Lumbreras, Pablo Valledor, Dafne Díaz-Jiménez, Luz Lestón, Javier Fernández, Azucena Mora   +6 more
doaj   +1 more source

Mobile colistin resistance (MCR), extended-spectrum beta-lactamase (ESBL) and multidrug resistance monitoring in Escherichia coli (commensal and pathogenic) in pig farming: need of harmonized guidelines and clinical breakpoints

Frontiers in Microbiology, 2022
Current data on antimicrobial resistance in pig production is essential for the follow-up strategic programs to eventually preserve the effectiveness of last-resort antibiotics for humans.
Vanesa García, Vanesa García, Isidro García-Meniño, Isidro García-Meniño, Verónica Gómez, Miguel Jiménez-Orellana, Antonio Méndez, Alvaro Aguarón, Elisabet Roca, Azucena Mora, Azucena Mora   +10 more
doaj   +1 more source

Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection

Molecular Systems Biology, 2006
We have systematically made a set of precisely defined, single‐gene deletions of all nonessential genes in Escherichia coli K‐12. Open‐reading frame coding regions were replaced with a kanamycin cassette flanked by FLP recognition target sites by using a
T. Baba, T. Ara, Miki Hasegawa, Yuki Takai, Yoshiko Okumura, Miki Baba, K. Datsenko, M. Tomita, B. Wanner, H. Mori   +9 more
semanticscholar   +1 more source