Results 51 to 60 of about 546,537 (214)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Serotype-dependent adhesion of Shiga toxin-producing Escherichia coli to bovine milk fat globule membrane proteins

Frontiers in Microbiology, 2022
Shiga toxin-producing Escherichia coli (STEC) are food-borne pathogens that can cause severe symptoms for humans. Raw milk products are often incriminated as vehicule for human STEC infection.
Arthur Bagel, Christelle Lopez, Elisabeth David-Briand, Valérie Michel, Thomas Douëllou, Delphine Sergentet, Delphine Sergentet   +6 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Chromatographic Analysis of Recombinant Lysostaphin Expressed in Escherichia coli [PDF]

, 2011
Lysostaphin (EC.3.4.24.75) is an extracellular glycylglycine endopeptidase produced exclusively by Staphylococcus simulans biovar staphylolyticus (ATCC 1362, NRRL B-2628).
Jennings, Claire
core  

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Microarray analysis of the ler regulon in enteropathogenic and enterohaemorrhagic escherichia coli strains [PDF]

, 2014
The type III protein secretion system is an important pathogenicity factor of enteropathogenic and enterohaemorrhagic Escherichia coli pathotypes. The genes encoding this apparatus are located on a pathogenicity island (the locus of enterocyte effacement)
Islam, Md. Shahidul, Islam, Shahidul, Mala Patel, Patel, Mala, Pallen, Mark J,, Busby, Stephen J W; id_orcid, Penn, Charles W, Pallen, Mark J., Bingle, Lewis, Charles W Penn, Lewis E H Bingle, Penn, C. W., Shaw, Robert K, Md Shahidul Islam, Lee, David J., Snyder, Lori A. S., Islam, Md Shahidul, Bingle, Lewis E. H., Busby Stephen J. W., Lori A S Snyder, Snyder Lori A. S., Busby, Stephen J. W., Constantinidou Chrystala, Mark J Pallen, Constantinidou, Chrystala, Snyder, Lori A S, Lee, David J, Penn Charles W., Pallen, Mark J, Islam Md. Shahidul, David J Lee, Robert K Shaw, Patel Mala, Bingle, Lewis E H, Chrystala Constantinidou, Stephen J W Busby, Penn, Charles W., Pallen Mark J., Lee David J., Bingle Lewis E. H., Shaw Robert K., Busby, Stephen J W, Shaw, Robert K.   +42 more
core   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Investigation of the source of haemolytic Escherichia coli infecting weaned pigs [PDF]

, 1987
Attempts were made to discover the source of strains of haemolytic Escherichia coli infecting weaned pigs on a piggery. The organisms were not detected in the faeces of sows in the farrowing house, or in the in the faeces or intestinal tracts of ...
Fu, Z.F., Robertson, I.D., Hampson, D.J.
core  

Prevalence and genetic characterization of shiga toxin-producing Escherichia coli isolates from slaughtered animals in Bangladesh [PDF]

, 2008
To determine the prevalence of Shiga toxin (Stx)-producing Escherichia coli (STEC) in slaughter animals in Dhaka, Bangladesh, we collected rectal contents immediately after animals were slaughtered.
Beumer, R.R., Boer, E., de, de Boer, E., Islam, M.A., Talukder, K.A., Zwietering, M.H., Mondol, A.S., Heuvelink, A.E.   +7 more
core   +1 more source