Molecular bases of circadian magnesium rhythms across eukaryotes
Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley +1 more source
Potential mechanism of action of J5 vaccine in protection against severe bovine coliform mastitis. [PDF]
Coliform mastitis is one of the most difficult diseases to treat in the modern dairy industry. Curative therapy with antibiotics remains only moderately effective and depends on the stage at which the disease is treated, The most successful strategies ...
Burvenich, Christian +2 more
core +3 more sources
New tools for recombinant protein production in Escherichia coli: A 5‐year update
The production of proteins in sufficient amounts is key for their study or use as biotherapeutic agents. Escherichia coli is the host of choice for recombinant protein production given its fast growth, easy manipulation, and cost‐effectiveness.
Germán L. Rosano +2 more
semanticscholar +1 more source
Peptide‐based ligand antagonists block a Vibrio cholerae adhesin
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang +9 more
wiley +1 more source
In Vitro Inhibition Zone Test Of Binahong (Anredera Cordifolia) Towards Staphylococcus Aureus, Enterococcus Faecalis, Escherichia Coli, And Pseudomonas Aeruginosa [PDF]
This is a true experimental research with post test-only control group design. The study was conducted to test the inhibitory zone of the Binahong leaf extract (Anredera cordifolia) against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli ...
Amertha, I. (IBPM) +2 more
core +2 more sources
Structure and genetics of Escherichia coli O antigens
Escherichia coli includes clonal groups of both commensal and pathogenic strains, with some of the latter causing serious infectious diseases. O antigen variation is current standard in defining strains for taxonomy and epidemiology, providing the basis ...
B. Liu +9 more
semanticscholar +1 more source
Escherichia coli Shapeshifters [PDF]
Published ahead of print 29 March 2013 The views expressed in this Commentary do not necessarily reflect the views of the journal or of ASM.
openaire +2 more sources
Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
Detection of Escherichia Coli Using PCR Analysis Without DNA Extraction
This study aimed to detect Escherichia coli directly without DNA extraction. The nucleus membrane and cell membranes of the Escherichia coli are composed of a phospholipid bilayer, damaged if heated at 950C.
Wimbuh Tri Widodo, Choirul Huda
doaj +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source

