Results 91 to 100 of about 1,293,873 (407)

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source

Comparison of bacterial intracellular and secreted proteins produced in milk versus medium for Escherichia coli by proteomic analysis

Journal of Dairy Science
: The growth and reproduction of microorganisms are dependent on nutrient supply. Here, milk and Luria-Bertani (LB) media were used as nutrition sources for Escherichia coli, and the changes in bacterial and secretory proteins at 3 time points (3, 9, and
Qiyue Sun, Yanxin Li, Hongning Jiang, Qijing Du, Jun Wang, Rongbo Fan, Rongwei Han, Yongxin Yang   +7 more
doaj   +1 more source

Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM).

PLoS ONE, 2019
Membrane microdomains or lipid rafts compartmentalize cellular processes by laterally organizing membrane components. Such sub-membrane structures were mainly described in eukaryotic cells, but, recently, also in bacteria.
José E Guzmán-Flores, Lidia Steinemann-Hernández, Luis E González de la Vara, Marina Gavilanes-Ruiz, Tony Romeo, Adrián F Alvarez, Dimitris Georgellis   +6 more
doaj   +1 more source

Label-free quantitative proteomic analysis of the inhibition effect of Lactobacillus rhamnosus GG on Escherichia coli biofilm formation in co-culture

Proteome Science, 2021
Background Escherichia coli (E. coli) is the principal pathogen that causes biofilm formation. Biofilms are associated with infectious diseases and antibiotic resistance.
Huiyi Song, Ni Lou, Jianjun Liu, Hong Xiang, Dong Shang   +4 more
doaj   +1 more source

Minimizing acetate formation in E. coli fermentations [PDF]

, 2007
Escherichia coli remains the best established production organisms in industrial biotechnology. However, during aerobic fermentation runs at high growth rates, considerable amounts of acetate are accumulated as by-product.
De Maeseneire, Sofie, De Mey, Marjan, Soetaert, Wim, Vandamme, Erick   +3 more
core   +2 more sources

Identifying New Small Proteins in Escherichia coli [PDF]

PROTEOMICS, 2018
Abstract The number of small proteins (SPs) encoded in the Escherichia coli genome is unknown, as current bioinformatics and biochemical techniques make short gene and small protein identification challenging.
Caitlin E. VanOrsdel, John P. Kelly, Brittany N. Burke, Christina D. Lein, Christopher E. Oufiero, Joseph F. Sanchez, Larry E. Wimmers, David J. Hearn, Fatimeh J. Abuikhdair, Kathryn R. Barnhart, Michelle L. Duley, Sarah E. G. Ernst, Briana A. Kenerson, Aubrey J. Serafin, Matthew R. Hemm   +14 more
openaire   +2 more sources

A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms

FEBS Open Bio, EarlyView.
We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

ArgR is an essential local transcriptional regulator of the arcABC-operon in Streptococcus suis and crucial for biological fitness in acidic environment [PDF]

, 2011
Streptococcus suis is one of the most important pathogens in pigs and can also cause severe infections in humans. Despite its clinical relevance very little is known about the factors contributing to its virulence.
Benga, L., Fulde, M., Goethe, R., Greeff, A., de, Smith, H.E., Valentin-Weigand, P., Willenborg, J.   +6 more
core   +2 more sources

Tuning Escherichia coli for membrane protein overexpression [PDF]

Proceedings of the National Academy of Sciences, 2008
A simple generic method for optimizing membrane protein overexpression in Escherichia coli is still lacking. We have studied the physiological response of the widely used “Walker strains” C41(DE3) and C43(DE3), which are derived from BL21(DE3), to membrane protein overexpression.
Wagner, Samuel, Klepsch, Mirjam M., Schlegel, Susan, Appel, Ansgar, Draheim, Roger, Tarry, Michael, Hogbom, Martin, van Wijk, Klaas J., Slotboom, Dirk J., Persson, Jan O., de Gier, Jan-Willem, Högbom, Martin   +11 more
openaire   +2 more sources