Results 91 to 100 of about 729,912 (260)

A P-type ATPase importer that discriminates between essential and toxic transition metals [PDF]

, 2009
Transition metals, although being essential cofactors in many physiological processes, are toxic at elevated concentrations. Among the membrane-embedded transport proteins that maintain appropriate intracellular levels of transition metals are ATP-driven
A. T. Lee, Agranoff, Arguello, Arg  ello, Axelsen, Bal, Bull, D. C. Rees, Finney, Francis, Grass, Johnson, Krogh, Legatzki, Lewinson, Melchers, Nies, O. Lewinson, Papp-Wallace, Phung, Rensing, Schafer, Sharma, Thompson, Trenor, Vulpe   +25 more
core   +2 more sources

Biogenesis of inner membrane proteins in Escherichia coli

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 2012
The inner membrane proteome of the model organism Escherichia coli is composed of inner membrane proteins, lipoproteins and peripherally attached soluble proteins. Our knowledge of the biogenesis of inner membrane proteins is rapidly increasing. This is in particular true for the early steps of biogenesis - protein targeting to and insertion into the ...
Joen Luirink, Zhong Yu, Jan-Willem de Gier, Samuel Wagner   +3 more
openaire   +4 more sources

Cloning of the C-terminal cytoplasmic fragment of the tar protein and effects of the fragment on chemotaxis of Escherichia coli [PDF]

, 1988
A gene encoding only the C-terminal portion of the receptor-transducer protein Tar of Escherichia coli was constructed. The gene product was detected and localized in the cytoplasmic fraction of the cell by immunoblotting with anti-Tar antibodies.
Mutoh, Norihiro, Oosawa, Kenji, Simon, M. I.   +2 more
core   +1 more source

Structural genomics of human proteins – target selection and generation of a public catalogue of expression clones

Microbial Cell Factories, 2005
Background The availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural genomics initiative, targets human proteins for structure ...
Harttig Ulrich, Sievert Volker, Scheich Christoph, Büssow Konrad, Schultz Jörg, Simon Bernd, Bork Peer, Lehrach Hans, Heinemann Udo   +8 more
doaj   +1 more source

MCE domain proteins: conserved inner membrane lipid-binding proteins required for outer membrane homeostasis

Scientific Reports, 2017
Bacterial proteins with MCE domains were first described as being important for Mammalian Cell Entry. More recent evidence suggests they are components of lipid ABC transporters.
Georgia L. Isom, Nathaniel J. Davies, Zhi-Soon Chong, Jack A. Bryant, Mohammed Jamshad, Maria Sharif, Adam F. Cunningham, Timothy J. Knowles, Shu-Sin Chng, Jeffrey A. Cole, Ian R. Henderson   +10 more
doaj   +1 more source

Consequences of Membrane Protein Overexpression in Escherichia coli

Molecular & Cellular Proteomics, 2007
Overexpression of membrane proteins is often essential for structural and functional studies, but yields are frequently too low. An understanding of the physiological response to overexpression is needed to improve such yields. Therefore, we analyzed the consequences of overexpression of three different membrane proteins (YidC, YedZ, and LepI) fused to
Anja Klußmeier, Jan-Willem de Gier, Olof Nord, Claudia Wagner, A. Jimmy Ytterberg, Per-Åke Nygren, Klaas J. van Wijk, Louise Baars, Samuel Wagner   +8 more
openaire   +4 more sources

Preparation of Outer Membrane Vesicle from Escherichia coli

Bio-Protocol, 2013
Outer membrane vesicles (OMVs) are spherical bilayered phospholipids of 20-200 nm in size produced from all Gram-negative bacteria and Gram-positive bacteria investigated to date.
Oh Kim, Bok Hong, Kyong-Su Park, Yae Yoon, Seng Choi, Won Lee, Tae-Young Roh, Yoon-Keun Kim, Yong Gho   +8 more
doaj   +1 more source

Calcium Homeostasis in Escherichia coli: Characterization of Mutants and Genome Expression of MG1655 [PDF]

, 2014
While the role of calcium ions as secondary messengers has been well described in eukaryotic cells, little is known about calcium homeostasis in bacteria at the physiological and molecular levels.
Arif, Muhammad, Howard, Josh, Tisa, Louis S.   +2 more
core   +1 more source

Assembly and overexpression of membrane proteins in Escherichia coli

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2003
The bacterium Escherichia coli is one of the most popular model systems to study the assembly of membrane proteins of the so-called helix-bundle class. Here, based on this system, we review and discuss what is currently known about the assembly of these membrane proteins. In addition, we will briefly review and discuss how E.
Jan-Willem de Gier, Linda Fröderberg, Louise Baars, David A. Drew   +3 more
openaire   +2 more sources

PROTEIN AND NUCLEIC ACID RELATIONSHIPS INESCHERICHIA COLIINFECTED WITH PHAGE T2 [PDF]

, 1958
Manuel Rosenbaum, William S. Preston
openalex   +1 more source