Producción de proteínas recombinantes de Plasmodium falciparum en Escherichia coli
Introducción. La producción de proteínas recombinantes es fundamental para el estudio funcional de las proteínas de Plasmodium falciparum. Sin embargo, las proteínas recombinantes de P.
Ángela Patricia Guerra+3 more
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Mutant MotB proteins in Escherichia coli [PDF]
The MotB protein of Escherichia coli is an essential component in each of eight torque generators in the flagellar rotary motor. Based on its membrane topology, it has been suggested that MotB might be a linker that fastens the torque-generating machinery to the cell wall.
Howard C. Berg, David F. Blair, D Y Kim
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Several proteins are expressed at too low abundance in the Escherichia coli (E. coli) proteome to be detected by standard methods. Here, the authors create a microfluidics-based platform enabling single-molecule counting of low-abundance proteins by ...
Burak Okumus+11 more
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Type III Secretion Effectors with Arginine N-Glycosyltransferase Activity [PDF]
Type III secretion systems are used by many Gram-negative bacterial pathogens to inject proteins, known as effectors, into the cytosol of host cells. These virulence factors interfere with a diverse array of host signal transduction pathways and cellular
Araujo Garrido, Juan Luis+2 more
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From ingestion to colonization: the influence of the host environment on regulation of the LEE encoded type III secretion system in enterohaemorrhagic Escherichia coli [PDF]
Enterohaemorrhagic Escherichia coli (EHEC) binds to host tissue and intimately attaches to intestinal cells using a dedicated type III secretion system (T3SS). This complex multi-protein organelle is encoded within a large pathogenicity island called the
Connolly, J.P., Finlay, B.B., Roe, A.J.
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Quantifying the benefit of a proteome reserve in fluctuating environments. [PDF]
The overexpression of proteins is a major burden for fast-growing bacteria. Paradoxically, recent characterization of the proteome of Escherichia coli found many proteins expressed in excess of what appears to be optimal for exponential growth.
Erickson, David W+4 more
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Escherichia coli-derived virus-like particles in vaccine development
Recombinant virus-like particle-based vaccines are composed of viral structural proteins and mimic authentic native viruses but are devoid of viral genetic materials. They are the active components in highly safe and effective vaccines for the prevention
Xiaofen Huang+4 more
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The production and detoxification of a potent cytotoxin, nitric oxide, by pathogenic enteric bacteria [PDF]
The nitrogen cycle is based on several redox reactions that are mainly accomplished by prokaryotic organisms, some archaea and a few eukaryotes, which use these reactions for assimilatory, dissimilatory or respiratory purposes.
Anke Arkenberg+36 more
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Protein abundance profiling of the Escherichia coli cytosol [PDF]
Knowledge about the abundance of molecular components is an important prerequisite for building quantitative predictive models of cellular behavior. Proteins are central components of these models, since they carry out most of the fundamental processes in the cell.
Dmitrij Frishman+9 more
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Isolate Specific Cold Response of Yersinia enterocolitica in Transcriptional, Proteomic, and Membrane Physiological Changes [PDF]
Yersinia enterocolitica, a zoonotic foodborne pathogen, is able to withstand low temperatures. This psychrotrophic ability allows it to multiply in food stored in refrigerators. However, little is known about the Y. enterocolitica cold response.
Alter, Thomas+4 more
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