Transcription-translation coupling: direct interactions of RNA polymerase with ribosomes and ribosomal subunits. [PDF]
In prokaryotes, RNA polymerase and ribosomes can bind concurrently to the same RNA transcript, leading to the functional coupling of transcription and translation.
Blaha, Gregor M+9 more
core +1 more source
Cloning of BMP-2 Gene and Its Expression Study in E. coli in Order to Produce a Recombinant Drug
Introduction & Objective: Bone morphogenetic proteins are a group of cytokines that belongs to superfamily TGFβ. These proteins play an important role in evolution of many of organs and tissues through germinal period followed by amending and rebuilding ...
Nejad Mohammadi+5 more
doaj
Expression and Purification of Mini G Proteins from Escherichia coli
Heterotrimeric G proteins modulate intracellular signalling by transducing information from cell surface G protein-coupled receptors (GPCRs) to cytoplasmic effector proteins.
Byron Carpenter, Christopher Tate
doaj +1 more source
Characterization of theEscherichia coliAntifungal Protein PPEBL21 [PDF]
An antifungal protein isolated fromEscherichia coliBL21 (PPEBL21) and predicted to be alcohol dehydrogenase (ADH) was subjected to biological characterization. The PPEBL21, indeed, demonstrated propionaldehyde-specific ADH activity. The Km and Vmax of PPEBL21 were found to be 644.8μM and 1.2 U/mg, respectively.
Vibha Yadav+4 more
openaire +4 more sources
Evolution of YidC/Oxa1/Alb3 insertases: three independent gene duplications followed by functional specialization in bacteria, mitochondria and chloroplasts [PDF]
Members of the YidC/Oxa1/Alb3 protein family facilitate the insertion, folding and assembly of proteins of the inner membranes of bacteria and mitochondria and the thylakoid membrane of plastids.
Funes, Soledad+4 more
core +1 more source
Recombinant protein production data after expression in the bacterium Escherichia coli
Fusion proteins have become essential for the expression and purification of recombinant proteins in Escherichia coli. The metal-binding protein CusF has shown several features that make it an attractive fusion protein and affinity tag: ''Expression and ...
J. Enrique Cantu-Bustos+5 more
doaj
Genomic island-encoded regulatory proteins in enterohemorrhagic Escherichia coli O157:H7
Enterohemorrhagic Escherichia coli (EHEC) is an important zoonotic pathogen that is a major cause of foodborne diseases in most developed and developing countries and can cause uncomplicated diarrhoea, haemorrhagic colitis, and haemolytic uraemic ...
Fang Wang+6 more
doaj +1 more source
Heterologous Expression and Purification of the CRISPR-Cas12a/Cpf1 Protein
This protocol provides step by step instructions (Figure 1) for heterologous expression of Francisella novicida Cas12a (previously known as Cpf1) in Escherichia coli.
P Mohanraju+3 more
doaj +1 more source
Background Escherichia coli (E. coli) is the principal pathogen that causes biofilm formation. Biofilms are associated with infectious diseases and antibiotic resistance.
Huiyi Song+4 more
doaj +1 more source
Inflating bacterial cells by increased protein synthesis. [PDF]
Understanding how the homeostasis of cellular size and composition is accomplished by different organisms is an outstanding challenge in biology. For exponentially growing Escherichia coli cells, it is long known that the size of cells exhibits a strong ...
Basan, Markus+6 more
core +2 more sources