Results 61 to 70 of about 1,293,873 (407)

ruvA Mutants that resolve Holliday junctions but do not reverse replication forks [PDF]

, 2008
RuvAB and RuvABC complexes catalyze branch migration and resolution of Holliday junctions (HJs) respectively. In addition to their action in the last steps of homologous recombination, they process HJs made by replication fork reversal, a reaction which ...
A Blastyak, A Constantinou, A Kuzminov, A Machwe, Alison Sylvia Bradley, B Guenther, B Michel, B Michel, Bénédicte Michel, C Ralf, CA Parsons, CE Shurvinton, CV Privezentzev, CZ Bachrati, D Chamberlain, D Hargreaves, DV Bugreev, EC Friedberg, EP Rocha, F Lecointe, F Osman, F Osman, G Grompone, H Iwasaki, H Shinagawa, IR Tsaneva, IR Tsaneva, Irina Tsaneva, JB Rafferty, JH Miller, JK Karow, JR Donaldson, K Hiom, K Yamada, K Yamada, L Wu, M Ariyoshi, M Fromant, M Lopes, M Oram, M Seigneur, Marie Le Masson, MC Whitby, MC Whitby, ME Robu, MJ Flores, P McGlynn, P McGlynn, P McGlynn, P McGlynn, R Lestini, RC Heller, RG Lloyd, SC West, SD Vincent, SM Roe, SN Chan, T Hishida, T Hishida, T Nishino, T Nishino, T Shibata, TN Mandal, W Bussen, William F. Burkholder, Y Liu, Y Liu, Z Baharoglu, Zeynep Baharoglu   +68 more
core   +3 more sources

Molecular bases of circadian magnesium rhythms across eukaryotes

FEBS Letters, EarlyView.
Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley   +1 more source

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Roles of transcriptional and translational control mechanisms in regulation of ribosomal protein synthesis in Escherichia coli [PDF]

, 2017
Bacterial ribosome biogenesis is tightly regulated to match nutritional conditions and to prevent formation of defective ribosomal particles. InEscherichia coli, most ribosomal protein (r-protein) synthesis is coordinated with rRNA synthesis by a ...
Burgos, Hector L, Gourse, Richard L, O\u27Connor, Kevin, Sanchez-Vazquez, Patricia   +3 more
core   +3 more sources

An adhesive protein capsule of Escherichia coli [PDF]

Infection and Immunity, 1985
The nature of the adhesive capacity of three hemagglutinating Escherichia coli strains that had earlier been described as nonfimbriated was studied. The strains that were isolated from human disease adhered to human buccal and urinary tract epithelial cells, an adhesion that was not inhibited by D-mannose.
I, Orskov, A, Birch-Andersen, J P, Duguid, J, Stenderup, F, Orskov   +4 more
openaire   +2 more sources

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Cloning, Expression, and Purification of α, βa, and βb Subunits of Human Inhibin Protein in Escherichia coli

Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, 2018
Background and Objectives: Inhibin is a glycoprotein hormone commonly found in the circulation, but its level increases in some diseases, and its measurement by serological method using anti-inhibin monoclonal antibodies, can help in diagnosis of some ...
Mohammad Ataei, Elahe Motevaseli, Mohammad Hossein Modarressi, Esmaeil Sadroddiny, Gholamreza Tavoosidana   +4 more
doaj  

Heterologous Expression and Purification of the CRISPR-Cas12a/Cpf1 Protein

Bio-Protocol, 2018
This protocol provides step by step instructions (Figure 1) for heterologous expression of Francisella novicida Cas12a (previously known as Cpf1) in Escherichia coli.
P Mohanraju, John Oost, Martin Jinek, Daan Swarts   +3 more
doaj   +1 more source

Proteomic Analysis of Duodenal Tissue from Escherichia coli F18-Resistant and -Susceptible Weaned Piglets. [PDF]

PLoS ONE, 2015
Diarrhea and edema disease in weaned piglets due to infection by Escherichia coli F18 is a leading cause of economic loss in the pig industry. Resistance to E.
Zhengchang Wu, Riwei Xia, Xuemei Yin, Yongjiu Huo, Guoqiang Zhu, Shenglong Wu, Wenbin Bao   +6 more
doaj   +1 more source

In vitro starch binding experiments: Study of the proteins related to grain hardness of wheat [PDF]

, 2007
Two friabilin components, puroindoline a and GSP-1 were expressed in Escherichia coli. Starch binding properties of the recombinant polypeptides and of friabilin extracted from wheat flour were compared in vitro. The produced proteins as well as native
Bakó, Ambrus, Gardonyi, M., Tamás, László   +2 more
core