Results 171 to 180 of about 5,623,526 (302)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Tetrahydrofuran Amino Acids of the Past Decade

Review: syntheses of THF α-, β-, γ-, δ- and longer chain amino acids; 78 ...
Turks, Māris, Rjabovs, Vitālijs
core  

Do pollinators play a role in shaping the essential amino acids found in nectar? [PDF]

New Phytol
Parkinson RH, Power EF, Walter K, McDermott-Roberts AE, Pattrick JG, Wright GA.   +5 more
europepmc   +2 more sources

Assessing gut microbial provisioning of essential amino acids to host in a mouse model with reconstituted gut microbiomes. [PDF]

Commun Biol
Ayayee P, Custer G, Clayton JB, Price J, Ramer-Tait A, Larsen T.   +5 more
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Rumen-Protected Amino Acids in Precision Nutrition of Dairy Cows: From Protection Methods to Production Efficiency

Scientific Papers Animal Science and Biotechnologies
Amino acids protected from ruminal degradation are an essential component of precision nutrition for ruminants, enabling the optimization of essential amino acid utilization under conditions of excessive ruminal degradation. Methionine, lysine, threonine,
Vasile-Cosmin Andronachi, Cristina Simeanu, Cristina-Gabriela Radu-Rusu, Vasile Vintilă, Daniel Simeanu   +4 more
doaj  

An Emergent Biosynthetic Pathway to Essential Amino Acids by Metabolic Metathesis. [PDF]

ACS Omega
Rivollier J, Gosling S, Pezo V, Heck MP, Marliere P.   +4 more
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Effects of lactic acid bacteria and grape seed tannin on enhancing intestinal supply of essential amino acids from alfalfa silage through improved preservation and rumen bypass in a multi-stage in vitro system. [PDF]

BMC Microbiol
Chen G, Deng J, Miao X, Bai C, Du H, Liu Q, Wang C, Chen L, Guo G, Huo W.   +9 more
europepmc   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source