Ethidium bromide binding to DNA cryogels
Abstract The interaction of the classical intercalator ethidium bromide (EtBr) with the double helical network strands of DNA cryogels was investigated. The cryogels were prepared starting from aqueous solutions of DNA (about 2000 base pairs long) at −18 °C using 1,4-butanediol diglycidyl ether crosslinker under various reaction conditions.
Karacan, Pinar, Okay, Oguz
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Ethidium bromide: Destruction and decontamination of solutions [PDF]
Ethidium bromide in water, TBE buffer, Mops buffer, and cesium chloride solution may be completely degraded by reaction with sodium nitrite and hypophosphorous acid. Only non-mutagenic reaction mixtures were produced. Destruction was greater than 99.8% in all cases; the limit of detection was 0.5 micrograms ethidium bromide per milliliter of solution ...
G, Lunn, E B, Sansone
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Agarose gel electrophoresis is a routinely employed experimental technique for visualizing nucleic acids, typically combined with ethidium bromide or some other molecular dye staining and a UV transilluminator.
Ryosuke Ishii
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Energetics and Mechanism of Drug Transport Mediated by the Lactococcal Multidrug Transporter LmrP [PDF]
The gene encoding the secondary multidrug transporter LmrP of Lactococcus lactis was heterologously expressed in Escherichia coli. The energetics and mechanism of drug extrusion mediated by LmrP were studied in membrane vesicles of E. coli. LmrP-mediated
Bolhuis, Henk, +6 more
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Ethidium Bromide Inhibits Mitochondrial Phosphorylating Oxidation
Ethidium bromide, in addition to combination with mitochondrial nucleic acids, is a phosphorylation inhibitor during glutamate and succinate respiration by mitochondria. Exhaustive washing of ethidium bromide-treated mitochondria did not relieve the inhibition nor significantly decrease the amount of bound dye.
E S, Higgins +3 more
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Transition on the entropic elasticity of DNA induced by intercalating molecules
We use optical tweezers to perform stretching experiments on DNA molecules when interacting with the drugs daunomycin and ethidium bromide, which intercalate the DNA molecule.
Ferreira, M. C. +2 more
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The red rain phenomenon of Kerala and its possible extraterrestrial origin [PDF]
A red rain phenomenon occurred in Kerala, India starting from 25th July 2001, in which the rainwater appeared coloured in various localized places that are spread over a few hundred kilometers in Kerala.
A. Santhosh Kumar +4 more
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Physicochemical analysis of rotavirus segment 11 supports a 'modified panhandle' structure and not the predicted alternative tRNA-like structure (TRLS) [PDF]
.Rotaviruses are a major cause of acute gastroenteritis, which is often fatal in infants. The viral genome consists of 11 double-stranded RNA segments, but little is known about their cis-acting sequences and structural elements.
A Heerschap +47 more
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HLA-G: expression in human keratinocytes in vitro and in human skin in vivo [PDF]
Classical, polymorphic major histocompatibility complex class I molecules are expressed on most nucleated cells.They present peptides at the cell surface and, thus, enable the immune system to scan peptides for their antigenicity.
Ulbrecht, M. +7 more
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Stochastic models (cooperative and non-cooperative) for NMR analysis of the hetero-association of aromatic molecules in aqueous solution [PDF]
Stochastic cooperative (STOCH-C) and non-cooperative (STOCH-NC) models have been developed for NMR analysis of the hetero-association of aromatic compounds in solution, in order to take into account all physically meaningful association reactions of ...
Davies, David B. +2 more
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