Results 1 to 10 of about 618,570 (321)

What happens to Bifidobacterium adolescentis and Bifidobacterium longum ssp. longum in an experimental environment with eukaryotic cells? [PDF]

open access: goldBMC Microbiology
Background The impact of probiotic strains on host health is widely known. The available studies on the interaction between bacteria and the host are focused on the changes induced by bacteria in the host mainly.
Dominika Jakubczyk   +9 more
doaj   +4 more sources

An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro [PDF]

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Transfection is a powerful analytical tool enabling studies of gene products and functions in eukaryotic cells. Successful delivery of genetic material into cells depends on DNA quantity and quality, incubation time and ratio of transfection reagent to ...
Agnieszka Fus-Kujawa   +7 more
doaj   +2 more sources

Autophagy in the Eukaryotic Cell [PDF]

open access: diamondEukaryotic Cell, 2002
The major cellular pathways for protein and organelle turnover are autophagy and proteasome-mediated degradation. These processes are important to maintain a well-controlled balance between anabolism and catabolism in order to have normal cell growth and development.
Fulvio Reggiori, Daniel J. Klionsky
openalex   +3 more sources

Engineered transcription-associated Cas9 targeting in eukaryotic cells [PDF]

open access: yesNature Communications
DNA targeting Class 2 CRISPR-Cas effector nucleases, including the well-studied Cas9 proteins, evolved protospacer-adjacent motif (PAM) and guide RNA interactions that sequentially license their binding and cleavage activities at protospacer target sites.
Gregory W. Goldberg   +11 more
doaj   +2 more sources

Bioenergetic myths of energy transduction in eukaryotic cells [PDF]

open access: yesFrontiers in Molecular Biosciences
The study of energy transduction in eukaryotic cells has been divided between Bioenergetics and Physiology, reflecting and contributing to a variety of Bioenergetic myths considered here: 1) ATP production = energy production, 2) energy transduction is ...
Guy C. Brown
doaj   +2 more sources

Interactions between Bacteriophages and Eukaryotic Cells [PDF]

open access: yesScientifica, 2020
As the name implies, bacteriophage is a bacterium-specific virus. It infects and kills the bacterial host. Bacteriophages have gained attention as alternative antimicrobial entities in the science community in the western world since the alarming rise of
Ramendra Dirgantara Putra   +1 more
doaj   +2 more sources

3-D Ultrastructure of O. tauri: Electron Cryotomography of an Entire Eukaryotic Cell [PDF]

open access: yes, 2007
The hallmark of eukaryotic cells is their segregation of key biological functions into discrete, membrane-bound organelles. Creating accurate models of their ultrastructural complexity has been difficult in part because of the limited resolution of light
A Al-Amoudi   +68 more
core   +27 more sources

Toxicity and virucidal activity of a neon-driven micro plasma jet on eukaryotic cells and a coronavirus. [PDF]

open access: greenFree Radic Biol Med, 2022
Mrochen DM   +11 more
europepmc   +3 more sources

Intrabody Expression in Eukaryotic Cells

open access: green, 2009
We describe procedures for intracellular expression of scFv in eukaryotic cells. Starting from a scFv gene cloned in a phage-display vector, we describe the cloning step into a mammalian expression vector, the transient transfection of a HeLa cell line, and the monitoring of intrabody expression by immunofluorescence staining and FACS analysis.
Laurence Guglielmi, Pierre Martineau
openalex   +5 more sources

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