An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 Transfection is a powerful analytical tool enabling studies of gene products and functions in eukaryotic cells. Successful delivery of genetic material into cells depends on DNA quantity and quality, incubation time and ratio of transfection reagent to ...
Agnieszka Fus-Kujawa +7 more
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Sphingolipid transport in eukaryotic cells [PDF]
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2000 Sphingolipids constitute a sizeable fraction of the membrane lipids in all eukaryotes and are indispensable for eukaryotic life. First of all, the involvement of sphingolipids in organizing the lateral domain structure of membranes appears essential for processes like protein sorting and membrane signaling.
Gerrit van Meer, Joost C. M. Holthuis
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Engineered transcription-associated Cas9 targeting in eukaryotic cells [PDF]
Nature CommunicationsDNA targeting Class 2 CRISPR-Cas effector nucleases, including the well-studied Cas9 proteins, evolved protospacer-adjacent motif (PAM) and guide RNA interactions that sequentially license their binding and cleavage activities at protospacer target sites.
Gregory W. Goldberg +11 more
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What happens to Bifidobacterium adolescentis and Bifidobacterium longum ssp. longum in an experimental environment with eukaryotic cells? [PDF]
BMC MicrobiologyBackground The impact of probiotic strains on host health is widely known. The available studies on the interaction between bacteria and the host are focused on the changes induced by bacteria in the host mainly.
Dominika Jakubczyk +9 more
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Bioenergetic myths of energy transduction in eukaryotic cells [PDF]
Frontiers in Molecular BiosciencesThe study of energy transduction in eukaryotic cells has been divided between Bioenergetics and Physiology, reflecting and contributing to a variety of Bioenergetic myths considered here: 1) ATP production = energy production, 2) energy transduction is ...
Guy C. Brown
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Interactions between Bacteriophages and Eukaryotic Cells [PDF]
Scientifica, 2020 As the name implies, bacteriophage is a bacterium-specific virus. It infects and kills the bacterial host. Bacteriophages have gained attention as alternative antimicrobial entities in the science community in the western world since the alarming rise of
Ramendra Dirgantara Putra +1 more
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Applications of Recombinant Dna Technology in Gastrointestinal Medicine and Hepatology: Basic Paradigms of Molecular Cell Biology. Part B: Eukaryotic Gene Transcription and Post-Transcripional Rna Processing [PDF]
Canadian Journal of Gastroenterology, 2000 The transcription of DNA into RNA is the primary level at which gene expression is controlled in eukaryotic cells. Eukaryotic gene transcription involves several different RNA polymerases that interact with a host of transcription factors to initiate ...
Gary E Wild +4 more
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Cellular structure and molecular functions of plants, animals, bacteria, and viruses [PDF]
Cellular, Molecular and Biomedical Reports, 2022 Plant cells are the basic unit of life in organisms of the kingdom Plantae. These organisms as eukaryotic cells have a true nucleus along with particular structures called organelles that perform various functions.
Hamid kheyrodin +2 more
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Eukaryotic Cell Capture by Amplified Magnetic in situ Hybridization Using Yeast as a Model
Frontiers in Microbiology, 2021 A non-destructive approach based on magnetic in situ hybridization (MISH) and hybridization chain reaction (HCR) for the specific capture of eukaryotic cells has been developed.
Fabiola Bastian +7 more
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Autophagy in the Eukaryotic Cell [PDF]
Eukaryotic Cell, 2002 The major cellular pathways for protein and organelle turnover are autophagy and proteasome-mediated degradation. These processes are important to maintain a well-controlled balance between anabolism and catabolism in order to have normal cell growth and development.
Reggiori, Fulvio, Klionsky, Daniel J
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