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Engineered transcription-associated Cas9 targeting in eukaryotic cells [PDF]
Nature CommunicationsDNA targeting Class 2 CRISPR-Cas effector nucleases, including the well-studied Cas9 proteins, evolved protospacer-adjacent motif (PAM) and guide RNA interactions that sequentially license their binding and cleavage activities at protospacer target sites.
Gregory W. Goldberg +11 more
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Interactions between Bacteriophages and Eukaryotic Cells [PDF]
Scientifica, 2020 As the name implies, bacteriophage is a bacterium-specific virus. It infects and kills the bacterial host. Bacteriophages have gained attention as alternative antimicrobial entities in the science community in the western world since the alarming rise of
Ramendra Dirgantara Putra +1 more
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An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro [PDF]
Frontiers in Bioengineering and Biotechnology, 2021 Transfection is a powerful analytical tool enabling studies of gene products and functions in eukaryotic cells. Successful delivery of genetic material into cells depends on DNA quantity and quality, incubation time and ratio of transfection reagent to ...
Agnieszka Fus-Kujawa +7 more
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What happens to Bifidobacterium adolescentis and Bifidobacterium longum ssp. longum in an experimental environment with eukaryotic cells? [PDF]
BMC MicrobiologyBackground The impact of probiotic strains on host health is widely known. The available studies on the interaction between bacteria and the host are focused on the changes induced by bacteria in the host mainly.
Dominika Jakubczyk +9 more
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Bioenergetic myths of energy transduction in eukaryotic cells [PDF]
Frontiers in Molecular BiosciencesThe study of energy transduction in eukaryotic cells has been divided between Bioenergetics and Physiology, reflecting and contributing to a variety of Bioenergetic myths considered here: 1) ATP production = energy production, 2) energy transduction is ...
Guy C. Brown
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Cellular structure and molecular functions of plants, animals, bacteria, and viruses [PDF]
Cellular, Molecular and Biomedical Reports, 2022 Plant cells are the basic unit of life in organisms of the kingdom Plantae. These organisms as eukaryotic cells have a true nucleus along with particular structures called organelles that perform various functions.
Hamid kheyrodin +2 more
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Autophagy in the Eukaryotic Cell [PDF]
Eukaryotic Cell, 2002 The major cellular pathways for protein and organelle turnover are autophagy and proteasome-mediated degradation. These processes are important to maintain a well-controlled balance between anabolism and catabolism in order to have normal cell growth and development.
Reggiori, Fulvio, Klionsky, Daniel J
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Frontiers in Cell and Developmental Biology, 2021 Cell synchronization is a powerful tool to understand cell cycle events and its regulatory mechanisms. Counter-flow centrifugal elutriation (CCE) is a more generally desirable method to synchronize cells because it does not significantly alter cell ...
Yongqiang Liu +12 more
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Eukaryotic Cell Capture by Amplified Magnetic in situ Hybridization Using Yeast as a Model
Frontiers in Microbiology, 2021 A non-destructive approach based on magnetic in situ hybridization (MISH) and hybridization chain reaction (HCR) for the specific capture of eukaryotic cells has been developed.
Fabiola Bastian +7 more
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Journal of Medical Microbiology and Infectious Diseases, 2023 Introduction: Developing potent therapeutic vaccines against human papillomaviruses (HPVs) is crucial for the effective management of various HPV-associated cancers.
Reyhane Najafi1 +3 more
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