Results 1 to 10 of about 10,696,119 (136)

An Overview of Methods and Tools for Transfection of Eukaryotic Cells in vitro

Frontiers in Bioengineering and Biotechnology, 2021
Transfection is a powerful analytical tool enabling studies of gene products and functions in eukaryotic cells. Successful delivery of genetic material into cells depends on DNA quantity and quality, incubation time and ratio of transfection reagent to ...
Agnieszka Fus-Kujawa, Pawel Prus, Pawel Prus, Karolina Bajdak-Rusinek, Paulina Teper, Katarzyna Gawron, Agnieszka Kowalczuk, Aleksander L. Sieron   +7 more
doaj   +2 more sources

Sae2/CtIP prevents R-loop accumulation in eukaryotic cells

eLife, 2018
The Sae2/CtIP protein is required for efficient processing of DNA double-strand breaks that initiate homologous recombination in eukaryotic cells. Sae2/CtIP is also important for survival of single-stranded Top1-induced lesions and CtIP is known to ...
Nodar Makharashvili, Sucheta Arora, Yizhi Yin, Qiong Fu, Xuemei Wen, Ji-Hoon Lee, Chung-Hsuan Kao, Justin WC Leung, Kyle M Miller, Tanya T Paull   +9 more
doaj   +2 more sources

3-D Ultrastructure of O. tauri: Electron Cryotomography of an Entire Eukaryotic Cell [PDF]

, 2007
The hallmark of eukaryotic cells is their segregation of key biological functions into discrete, membrane-bound organelles. Creating accurate models of their ultrastructural complexity has been difficult in part because of the limited resolution of light
A Al-Amoudi, A Briegel, A Komeili, A Scheffel, AM van de Meene, AS Frangakis, AS Frangakis, B Farinas, B Palenik, B Storrie, C Courties, C Courties, C Suloway, CA Mannella, CA Mannella, CA Mannella, CA Mannella, CM Spahn, CV Iancu, CV Iancu, CW Akey, D Hoepfner, D Nicastro, D Stoffler, DN Mastronarde, E Derelle, E Shimoni, ER Wright, G Perkins, GC Johnston, GJ Jensen, GK Voeltz, GM Gaietta, GP Henderson, Grant J. Jensen, Gregory P. Henderson, HS Yoon, J Dubochet, J Kurner, JB Heymann, JL Hoog, JP Caviston, JP Ral, JR Warner, KL McDonald, LA Staehelin, LR Comolli, Lu Gan, M Beck, M Marko, MJ Chrétiennot-Dinet, O Medalia, PA Leong, Paul Digard, PE Purdue, Q Yang, QS Zheng, R Grimm, R Nevo, R Zadrag, RR Guillard, RT Mullen, S Robbens, S Subramaniam, SP Drummond, UC Vothknecht, V Lucic, V Stoppin, Y Shibata   +68 more
core   +20 more sources

Cellular structure and molecular functions of plants, animals, bacteria, and viruses [PDF]

Cellular, Molecular and Biomedical Reports, 2022
Plant cells are the basic unit of life in organisms of the kingdom Plantae. These organisms as eukaryotic cells have a true nucleus along with particular structures called organelles that perform various functions.
Hamid kheyrodin, Raheba Jami, Fazal Rehman   +2 more
doaj   +1 more source

An Optimized and Versatile Counter-Flow Centrifugal Elutriation Workflow to Obtain Synchronized Eukaryotic Cells

Frontiers in Cell and Developmental Biology, 2021
Cell synchronization is a powerful tool to understand cell cycle events and its regulatory mechanisms. Counter-flow centrifugal elutriation (CCE) is a more generally desirable method to synchronize cells because it does not significantly alter cell ...
Yongqiang Liu, Yongqiang Liu, Yongqiang Liu, Bei Nan, Bei Nan, Bei Nan, Junhua Niu, Junhua Niu, Junhua Niu, Geoffrey M. Kapler, Shan Gao, Shan Gao, Shan Gao   +12 more
doaj   +1 more source

Eukaryotic Cell Capture by Amplified Magnetic in situ Hybridization Using Yeast as a Model

Frontiers in Microbiology, 2021
A non-destructive approach based on magnetic in situ hybridization (MISH) and hybridization chain reaction (HCR) for the specific capture of eukaryotic cells has been developed.
Fabiola Bastian, Delphine Melayah, Mylène Hugoni, Mylène Hugoni, Nora M. Dempsey, Pascal Simonet, Marie Frenea-Robin, Laurence Fraissinet-Tachet   +7 more
doaj   +1 more source

A comparative analysis of miRNA expression in human lung epithelial cells during infection with influenza virus and RNAse treatment

Инфекция и иммунитет, 2022
The influenza virus is capable of causing an acute respiratory infection that affects 5 to 20% of the human population annually. The spread of the influenza virus epidemic occurs within a short period of time due to its high contagiousness.
Irina A. Baichurina, M. I. Markelova, R. Shah Mahmud   +2 more
doaj   +1 more source

Analytical distributions for detailed models of stochastic gene expression in eukaryotic cells

Proceedings of the National Academy of Sciences of the United States of America, 2020
Significance The random nature of gene expression is well established experimentally. Mathematical modeling provides a means of understanding the factors leading to the observed stochasticity.
Zhixing Cao, R. Grima
semanticscholar   +1 more source

Antibacterial Peptide BSN-37 Kills Extra- and Intra-Cellular Salmonella enterica Serovar Typhimurium by a Nonlytic Mode of Action

Frontiers in Microbiology, 2020
The increasing rates of resistance to traditional anti-Salmonella agents have made the treatment of invasive salmonellosis more problematic, which necessitates the search for new antimicrobial compounds.
Lei Yang, Yawei Sun, Yanzhao Xu, Bolin Hang, Lei Wang, Ke Zhen, Bing Hu, Yanan Chen, Xiaojing Xia, Jianhe Hu   +9 more
doaj   +1 more source

Flexible, Biocompatible PET Sheets: A Platform for Attachment, Proliferation and Differentiation of Eukaryotic Cells

Surfaces, 2021
Transparent, flexible, biaxially oriented polyethylene terephthalate (PET) sheets were modified by bioactive polymer-fibronectin top layers for the attachment of cells and growth of muscle fibers.
Soumen Samanta, Diana Gaad, Eva Cabet, Alain Lilienbaum, Ajay Singh, Dinesh K. Aswal, Mohamed M. Chehimi   +6 more
doaj   +1 more source