Results 11 to 20 of about 387,698 (305)

Functional capacity of Shiga-toxin promoter sequences in eukaryotic cells. [PDF]

PLoS ONE, 2013
Shiga toxins (Stx) are the main virulence factors in enterohemorrhagic Escherichia coli (EHEC) infections, causing diarrhea and hemolytic uremic syndrome (HUS). The genes encoding for Shiga toxin-2 (Stx2) are located in a bacteriophage.
Leticia V Bentancor, Marcos F Bilen, María P Mejías, Romina J Fernández-Brando, Cecilia A Panek, Maria V Ramos, Gabriela C Fernández, Martín Isturiz, Pablo D Ghiringhelli, Marina S Palermo   +9 more
doaj   +4 more sources

Intrabody Expression in Eukaryotic Cells [PDF]

, 2009
We describe procedures for intracellular expression of scFv in eukaryotic cells. Starting from a scFv gene cloned in a phage-display vector, we describe the cloning step into a mammalian expression vector, the transient transfection of a HeLa cell line, and the monitoring of intrabody expression by immunofluorescence staining and FACS analysis.
Guglielmi, Laurence, Martineau, Pierre
openaire   +4 more sources

Directed Signaling Cascades in Monodisperse Artificial Eukaryotic Cells. [PDF]

ACS Nano, 2021
The bottom-up assembly of multi-compartment artificial cells that are able to direct biochemical reactions along a specific spatial pathway remains a considerable engineering challenge. In this work, we address this with a microfluidic platform which is
Shetty SC, Yandrapalli N, Pinkwart K, Krafft D, Vidakovic-Koch T, Ivanov I, Robinson T.   +6 more
europepmc   +2 more sources

Antibacterial Peptide BSN-37 Kills Extra- and Intra-Cellular Salmonella enterica Serovar Typhimurium by a Nonlytic Mode of Action

Frontiers in Microbiology, 2020
The increasing rates of resistance to traditional anti-Salmonella agents have made the treatment of invasive salmonellosis more problematic, which necessitates the search for new antimicrobial compounds.
Lei Yang, Yawei Sun, Yanzhao Xu, Bolin Hang, Lei Wang, Ke Zhen, Bing Hu, Yanan Chen, Xiaojing Xia, Jianhe Hu   +9 more
doaj   +1 more source

Flexible, Biocompatible PET Sheets: A Platform for Attachment, Proliferation and Differentiation of Eukaryotic Cells

Surfaces, 2021
Transparent, flexible, biaxially oriented polyethylene terephthalate (PET) sheets were modified by bioactive polymer-fibronectin top layers for the attachment of cells and growth of muscle fibers.
Soumen Samanta, Diana Gaad, Eva Cabet, Alain Lilienbaum, Ajay Singh, Dinesh K. Aswal, Mohamed M. Chehimi   +6 more
doaj   +1 more source

A comparative analysis of miRNA expression in human lung epithelial cells during infection with influenza virus and RNAse treatment

Инфекция и иммунитет, 2022
The influenza virus is capable of causing an acute respiratory infection that affects 5 to 20% of the human population annually. The spread of the influenza virus epidemic occurs within a short period of time due to its high contagiousness.
Irina A. Baichurina, M. I. Markelova, R. Shah Mahmud   +2 more
doaj   +1 more source

The archaeo-eukaryotic GINS proteins and the archaeal primase catalytic subunit PriS share a common domain [PDF]

, 2010
This work was funded by the Scottish Universities Life Sciences Alliance (SULSA).Primase and GINS are essential factors for chromosomal DNA replication in eukaryotic and archaeal cells.
Agnieszka Swiatek, Swiatek Agnieszka, MacNeill, Stuart Andrew, MacNeill Stuart A, Stuart A MacNeill, Swiatek, Agnieszka   +5 more
core   +1 more source

The Plasmodium eukaryotic initiation factor-2α kinase IK2 controls the latency of sporozoites in the mosquito salivary glands [PDF]

, 2010
Sporozoites, the invasive form of malaria parasites transmitted by mosquitoes, are quiescent while in the insect salivary glands. Sporozoites only differentiate inside of the hepatocytes of the mammalian host. We show that sporozoite latency is an active
Sullivan, W. J., Sullivan, William J., Sakthivel, Ramanavelan, Fontoura, Beatriz M. A., Doerig, C., Winzeler, Elizabeth A., Tuteja, R., Roos, David S., Gueirard, Pascale, Ruth S. Nussenzweig, Tuteja, Renu, Anat Caspi, Robert Ménard, Meister, S., Ranford-Cartwright, L., Ranford-Cartwright, Lisa, Lisa Ranford-Cartwright, Nussenzweig, Victor, Ramanavelan Sakthivel, Nussenzweig, V., Ranford-Cartwright, L.C., Clare Fennell, Min Zhang, Sakthivel, R., Fennell, Clare, Nussenzweig, R. S., Sullivan, William J., Jr., Caspi, A., Beatriz M.A. Fontoura, Gueirard, P., Renu Tuteja, Menard, R., William J. Sullivan, Pascale Gueirard, Ménard, Robert, Meister, Stephan, Stephan Meister, Victor Nussenzweig, Doerig, Christian, Fontoura, B. M. A., David S. Roos, Zhang, Min, Roos, D. S., Christian Doerig, Fennell, C., Winzeler, E. A., Caspi, Anat, Zhang, M., Elizabeth A. Winzeler, Nussenzweig, Ruth S.   +49 more
core   +1 more source

Isolation of Myricitrin and 3,5-di-O-Methyl Gossypetin from Syzygium samarangense and Evaluation of their Involvement in Protecting Keratinocytes against Oxidative Stress via Activation of the Nrf-2 Pathway

Molecules, 2019
The wax apple (Syzygium samarangense) is traditionally employed as an antibacterial and immunostimulant drug in traditional medicine. This plant is rich in different flavonoids and tannins. In this study, we isolated two compounds from S.
Mansour Sobeh, Ganna Petruk, Samir Osman, Mohamed A. El Raey, Paola Imbimbo, Daria Maria Monti, Michael Wink   +6 more
doaj   +1 more source

DNAReplication: a database of information and resources for the eukaryotic DNA replication community [PDF]

, 2009
DNAReplication (at http://www.dnareplication.net) has been set up as a freely available single resource to facilitate access to information on eukaryotic DNA replication.
Cotterill, S, Kearsey, Stephen E., Kearsey, SE, Kearsey, Stephen, Cotterill, Sue   +4 more
core   +2 more sources