Results 171 to 180 of about 22,111 (197)

The vaccinia virus E3L dsRNA binding protein detects distinct production patterns of exogenous and endogenous dsRNA

2023
AbstractDouble-stranded RNA (dsRNA) is a pathogen associated molecular pattern recognized by multiple pattern recognition receptors and induces innate immune responses. Viral infections can generate dsRNA during virus replication. Genetic mutations can also lead to endogenous dsRNA accumulation.
Wenjing Zhang, Nikhila S. Tanneti, Alejandra Fausto, Joangela Nouel, Hanako Reyes, Susan R. Weiss, Yize Li   +6 more
openaire   +2 more sources

Temporary silencing targeted plant genes with exogenous application of dsRNA

Acta Horticulturae, 2020
Silencing of plant genes has been a stable technique in plant molecular biology however the two best methods at the moment are either permanently transforming the plant, which results in a genetically modified organism, or transient silencing induced by viral-induced gene silencing (VIGS), which relies on a compatible viral vector.
W. Nak, A. Hayward, M. Gleeson, N. Mitter   +3 more
openaire   +1 more source

Exogenous administration of dsRNA for the demonstration of RNAi in Maruca vitrata (lepidoptera: crambidae)

3 Biotech, 2021
The polyphagous spotted pod borer, Maruca vitrata is an important agricultural pest that causes extensive damage on various food crops. Though the pest is managed by synthetic chemicals, exploration of biotechnological approaches for its control is important.
Madhurima Chatterjee, Jyoti Yadav, Maniraj Rathinam, Abhishek Mandal, Gopal Chowdhary, Rohini Sreevathsa, Uma Rao   +6 more
openaire   +2 more sources

RNA‐dependent RNA polymerase could extend the lasting validity period of exogenous dsRNA

Pest Management Science, 2022
AbstractBACKGROUNDPrevious studies have found that pesticide double‐stranded (ds)RNA usually has a long‐lasting validity period in plants. However, it is uncertain if any factors in plants could extend dsRNA duration. It has been reported that RNA‐dependent RNA polymerases (RdRP) in plants and some other eukaryotes could catalyze RNA amplification and ...
Hainan Zhang, Jing Gao, Jiasheng Chen, Yue Peng, Zhaojun Han   +4 more
openaire   +2 more sources

Exogenous dsRNA trigger RNAi in Venturia inaequalis resulting in down regulation of target genes and growth reduction

Molecular Biology Reports, 2023
Venturia inaequalis is an apple scab causing fungal pathogen. It is a highly contagious and destructive pathogen which rapidly spreads infection in the surrounding orchards if not managed. The management and control of disease require multiple fungicides to be sprayed at different development stages of the apple.
Suhani Bhagta, Vinay Bhardwaj, Anil Kant
openaire   +2 more sources

Citrus exosome-modified exogenous dsRNA delivery reduces plant pathogen resistance and mycotoxin production

Pesticide Biochemistry and Physiology
Plant-derived exosome-like nanoparticles (PENs) are crucial for intercellular communication. However, PEN-based transport of pathogenic fungal genes remains unclear. This study isolated and purified PENs from lane late navel orange citrus juice by following the sucrose gradient ultracentrifugation technique.
Chunxiao, Yin, Yuli, Lao, Lihong, Xie, Lianfei, Chen, Yueming, Jiang, Liang, Gong   +5 more
openaire   +2 more sources

Expression of exogenous dsRNA by Lecanicillium attenuatum enhances its virulence to Dialeurodes citri

Pest Management Science, 2018
AbstractBACKGROUNDDialeurodes citri is an important pest in citrus‐producing areas of the world. Lecanicillium attenuatum parasitizes D. citri and kills it, suggesting a potential approach for the biological control of pests. However, the low virulence of the fungus and its slow rate of killing have limited its commercial competitiveness. The objective
Shi‐Jiang Yu, Qi Pan, Ren Luo, Cui‐Lun Wang, Lu‐Yan Cheng, Juan‐Sheng Yang, Hao‐Nan Zhou, Dong‐Yuan Hou, Hao‐Qiang Liu, Chun Ran   +9 more
openaire   +2 more sources

Optimal impulsive control in RNA interference mediated by exogenous dsRNA with physiological delays

International Journal of Biomathematics
Therapeutic agents acting on RNA, including RNA modification, RNA editing and RNA interference (RNAi), play a vital role in gene function study, signaling pathway, drug discovery, disease treatment, vaccine development and so on. Therein, RNAi as an emerging gene therapy has been widely applied in many cancer studies by silencing oncogenes or specific
Changguo Li, Yongzhen Pei, Zhenzhen Liu, Ruimin Zhang   +3 more
openaire   +1 more source

Cellular dsRNA interactome reveals the regulatory map of exogenous RNA sensing

SUMMARYRNA-binding proteins (RBPs) provide a critical post-transcriptional regulatory layer in determining RNA fate. Currently, UV crosslinking followed by oligo-dT pull-down is the golden standard in identifying the RBP repertoire, but such method is ineffective in capturing RBPs that recognize double-stranded RNAs (dsRNAs).
JinA Lim, Namseok Lee, Seonmin Ju, Jeesoo Kim, Subin Mun, Moonhyeon Jeon, Yong-ki Lee, Seok-Hoon Lee, Jayoung Ku, Sujin Kim, Sangsu Bae, Jong-Seo Kim, Yoosik Kim   +12 more
openaire   +1 more source

Exogenous dsRNA made accessible to Dicer by two eukaryotic RNA-dependent RNA polymerases

Abstract Discrimination of self from non-self RNA is a critical requirement for any cell to respond to infections and to maintain cellular integrity. We report novel functions for two RNA-dependent RNA polymerases (RDRs) inParameciumin the detection of exogenous RNA. In the RNA interference (RNAi) mechanism, RDRs are normally involved in the production
Marcello Pirritano, Johannes Buescher, Pauline Staubach, Thorsten Tacken, Yulia Yakovleva, Mark Sabura, Kristela Shehu, Sören Franzenburg, Marc Schneider, Martin Simon   +9 more
openaire   +1 more source