Results 41 to 50 of about 3,047 (148)

Direct observation of processive exoribonuclease motion using optical tweezers [PDF]

Proceedings of the National Academy of Sciences, 2015
Significance Bacteria regulate the synthesis and degradation of RNA molecules to ensure timely and robust responses to an ever-changing environment. The transcript’s lifetime can be influenced profoundly by a secondary structure that can form in the RNA and that may inhibit or promote its digestion by RNases.
Furqan M, Fazal, Daniel J, Koslover, Ben F, Luisi, Steven M, Block   +3 more
openaire   +2 more sources

Non‐Targeting shRNA‐Encoded Plasmid DNA Enhances Protective Immunity Through RIDD‐RIG‐I Signaling Pathway in the Zika Virus Animal Model

Advanced Science, EarlyView.
We present a novel DNA vaccine platform featuring intrinsic, non‐targeting dsRNA that significantly enhances immune responses by activating the regulated IRE1‐dependent decay‐RIG‐I signaling pathway. This research elucidates a previously uncharacterized mechanism for dsRNA‐mediated innate immune activation.
Min‐Syuan Huang, Hung‐Chun Liao, Po Peng, Wan‐Ling Wu, Kit Man Chai, Mei‐Yu Chen, Guann‐Yi Yu, Tsung‐Hsien Chuang, Hsin‐Wei Chen, Chuang‐Rung Chang, Shih‐Jen Liu   +10 more
wiley   +1 more source

A novel fry1 allele reveals the existence of a mutant phenotype unrelated to 5'->3' exoribonuclease (XRN) activities in Arabidopsis thaliana roots. [PDF]

PLoS ONE, 2011
Mutations in the FRY1/SAL1 Arabidopsis locus are highly pleiotropic, affecting drought tolerance, leaf shape and root growth. FRY1 encodes a nucleotide phosphatase that in vitro has inositol polyphosphate 1-phosphatase and 3',(2'),5'-bisphosphate ...
Judith Hirsch, Julie Misson, Peter A Crisp, Pascale David, Vincent Bayle, Gonzalo M Estavillo, Hélène Javot, Serge Chiarenza, Allison C Mallory, Alexis Maizel, Marie Declerck, Barry J Pogson, Hervé Vaucheret, Martin Crespi, Thierry Desnos, Marie-Christine Thibaud, Laurent Nussaume, Elena Marin   +17 more
doaj   +1 more source

The coronavirus proofreading exoribonuclease mediates extensive viral recombination

PLOS Pathogens, 2020
Recombination is proposed to be critical for coronavirus (CoV) diversity and emergence of SARS-CoV-2 and other zoonotic CoVs. While RNA recombination is required during normal CoV replication, the mechanisms and determinants of CoV recombination are not known.
Jennifer Gribble, Laura J. Stevens, Maria L. Agostini, Jordan Anderson-Daniels, James D. Chappell, Xiaotao Lu, Andrea J. Pruijssers, Andrew L. Routh, Mark R. Denison   +8 more
openaire   +4 more sources

Regulation of R‐Loop Dynamics by Proteins and Long Noncoding RNAs: An Emerging Paradigm for Cancer Treatment

Cancer Science, EarlyView.
R‐loops are three‐stranded nucleic acid structures whose dysregulation leads to genomic instability and cancer progression. This review summarizes the protein and lncRNA machineries that regulate R‐loop dynamics and discusses the therapeutic potential of targeting these pathways in cancer.
Miho M. Suzuki, Keiko Shinjo, Tatsunori Nishimura, Yutaka Kondo   +3 more
wiley   +1 more source

Human FASTK preferentially binds single‐stranded and G‐rich RNA

The FEBS Journal, EarlyView.
Although FASTK is known to play a role in mRNA biology, the mechanism through which it recognizes RNA has yet to be unraveled. Here, we used purified human FASTK to characterize its RNA‐binding properties in vitro. We found that FASTK prefers ssRNA oligonucleotides containing guanines with the potential to form G‐quadruplexes and binds robustly to any ...
Daria M. Dawidziak, Dawid A. Dzadz, Mikołaj I. Kuska, Madhuri Kanavalli, Maria M. Klimecka, Matthew Merski, Katarzyna J. Bandyra, Maria W. Górna   +7 more
wiley   +1 more source

RISC-interacting clearing 3’- 5’ exoribonucleases (RICEs) degrade uridylated cleavage fragments to maintain functional RISC in Arabidopsis thaliana

eLife, 2017
RNA-induced silencing complex (RISC) is composed of miRNAs and AGO proteins. AGOs use miRNAs as guides to slice target mRNAs to produce truncated 5' and 3' RNA fragments. The 5' cleaved RNA fragments are marked with uridylation for degradation.
Zhonghui Zhang, Fuqu Hu, Min Woo Sung, Chang Shu, Claudia Castillo-González, Hisashi Koiwa, Guiliang Tang, Martin Dickman, Pingwei Li, Xiuren Zhang   +9 more
doaj   +1 more source

Exoribonuclease superfamilies: structural analysis and phylogenetic distribution

Nucleic Acids Research, 2001
Exoribonucleases play an important role in all aspects of RNA metabolism. Biochemical and genetic analyses in recent years have identified many new RNases and it is now clear that a single cell can contain multiple enzymes of this class. Here, we analyze the structure and phylogenetic distribution of the known exoribonucleases.
Y, Zuo, M P, Deutscher
openaire   +3 more sources

Suppression of Viral RNA Recombination by a Host Exoribonuclease [PDF]

Journal of Virology, 2006
ABSTRACT RNA viruses of humans, animals, and plants evolve rapidly due to mutations and RNA recombination. A previous genome-wide screen in Saccharomyces cerevisiae , a model host, identified five host genes, including XRN1 , encoding a 5′-3′ exoribonuclease, whose absence led to an
Chi-Ping, Cheng, Elena, Serviene, Peter D, Nagy   +2 more
openaire   +2 more sources

Modulation of SAL retrograde signalling promotes yield and water productivity responses in dynamic field environments

New Phytologist, Volume 249, Issue 3, Page 1219-1233, February 2026.
Summary Chloroplast‐to‐nucleus retrograde signalling enables rapid stress responses in plants, but whether these signals accumulate to affect crop performance across entire growing seasons under field conditions remains unknown. We generated wheat mutants with targeted deletions in specific SAL gene copies from two distinct homeologous groups (TaSAL1 ...
Andrew F. Bowerman, Marten Moore, Arun Yadav, Jing Zhang, Matthew D. Mortimer, Zuzana Plšková, Estee E. Tee, Eng Kee Au, Derek P. Collinge, Gonzalo M. Estavillo, Crispin A. Howitt, Kai X. Chan, Greg J. Rebetzke, Barry J. Pogson   +13 more
wiley   +1 more source