Results 31 to 40 of about 3,495 (190)
Synthesis of 5’ end-labeled RNA
Bio-Protocol, 2012 5’ end-labeled RNA molecules are useful substrates to analyse the endo- and exonucleolytic activities of various ribonucleases. Here two protocols are given to synthesize P32 labeled RNAs with a 5’ PPP or 5’ P moiety. 5’ exoribonucleases generally do not
Harald Putzer
doaj +1 more source
Nuclease Activity of the Junín Virus Nucleoprotein C-Terminal Domain
Viruses, 2023 The mammarenavirus Junín (JUNV) is the causative agent of Argentine hemorrhagic fever, a severe disease of public health concern. The most abundant viral protein is the nucleoprotein (NP), a multifunctional, two-domain protein with the primary role as ...
Alicia Armella Sierra +6 more
doaj +1 more source
Characterization of a bafinivirus exoribonuclease activity
Journal of General Virology, 2018 White bream virus (WBV), a poorly characterized plus-strand RNA virus infecting freshwater fish of the Cyprinidae family, is the prototype species of the genus Bafinivirus in the subfamily Torovirinae (family Coronaviridae, order Nidovirales). In common with other nidoviruses featuring >20 kilobase genomes, bafiniviruses have been predicted to encode ...
Izabela, Durzynska +4 more
openaire +2 more sources
BMC Genomics, 2018 Background Exoribonucleases are crucial for RNA degradation in Escherichia coli but the roles of RNase R and PNPase and their potential overlap in stationary phase are not well characterized.
Clémentine Dressaire +5 more
doaj +1 more source
Multiple Exoribonucleases Catalyze Maturation of the 3′ Terminus of 16S Ribosomal RNA (rRNA) [PDF]
, 2013 Background: The RNases involved in 3′ maturation of E. coli 16S rRNA were not known. Results: E. coli mutants lacking RNase II, RNase R, PNPase, and RNase PH accumulate 17S rRNA precursor.
Deutscher, Murray P, Sulthana, Shaheen
core +1 more source
Structural characterization of NrnC identifies unifying features of diribonucleotidases [PDF]
, 2021 RNA degradation is fundamental for cellular homeostasis. The process is carried out by various classes of endolytic and exolytic enzymes that together degrade an RNA polymer to mono-ribonucleotides.
Lormand, Justin D. +8 more
core +1 more source
RNase J1 and J2 Are Host-Encoded Factors for Plasmid Replication
Frontiers in Microbiology, 2021 Plasmids need to ensure their transmission to both daughter-cells when their host divides, but should at the same time avoid overtaxing their hosts by directing excessive host-resources toward production of plasmid factors.
Vanessa Andrade Guimarães +10 more
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Degradation of microRNAs by a Family of Exoribonucleases in Arabidopsis [PDF]
Science, 2008 microRNAs (miRNAs) play crucial roles in numerous developmental and metabolic processes in plants and animals. The steady-state levels of miRNAs need to be properly controlled to ensure normal development. Whereas the framework of miRNA biogenesis is established, factors involved in miRNA degradation remain unknown.
Vanitharani, Ramachandran, Xuemei, Chen
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A folded viral noncoding RNA blocks host cell exoribonucleases through programmed remodeling of RNA structure [PDF]
, 2018 Folded RNA elements that block processive 5′→3′ cellular exoribonucleases (xrRNAs) to produce biologically active viral non-coding RNAs were discovered in flaviviruses, potentially revealing a new mode of RNA maturation.
Anna-Lena Steckelberg +5 more
core +1 more source
Cell Reports, 2019 Summary: Cell-based studies of human ribonucleases traditionally rely on methods that deplete proteins slowly. We engineered cells in which the 3′→5′ exoribonucleases of the exosome complex, DIS3 and EXOSC10, can be rapidly eliminated to assess their ...
Lee Davidson +7 more
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