Results 71 to 80 of about 2,813,323 (292)

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source

Human and Extracellular DNA Depletion for Metagenomic Analysis of Complex Clinical Infection Samples Yields Optimized Viable Microbiome Profiles

Cell Reports, 2019
Summary: Metagenomic sequencing is a promising approach for identifying and characterizing organisms and their functional characteristics in complex, polymicrobial infections, such as airway infections in people with cystic fibrosis.
Maria T. Nelson, Christopher E. Pope, Robyn L. Marsh, Daniel J. Wolter, Eli J. Weiss, Kyle R. Hager, Anh T. Vo, Mitchell J. Brittnacher, Matthew C. Radey, Hillary S. Hayden, Alexander Eng, Samuel I. Miller, Elhanan Borenstein, Lucas R. Hoffman   +13 more
doaj   +1 more source

Extracellular DNA extraction v1 [PDF]

, 2020
Over the past decade, an increasing number of studies has used environmental DNA from lake sediments to trace past lake ecosystem and landscape changes, agricultural activities or human presence and more broadly the biodiversity. In the environment, DNA can be found as intracellular and extracellular DNA (iDNA and exDNA).
Charline Giguet-Covex, Pierre Taberlet, Francesco Gentile Ficetola   +2 more
openaire   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Extracellular DNA in bacterial biofilms. Part I: origin

Annals of Mechnikov's Institute, 2020
Significant number of chronic bacterial infections involves the biofilm formation, but regulation of this process is still far from beingwell understood.
S Krestetska
doaj  

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Extracellular DNA Traps: Origin, Function and Implications for Anti-Cancer Therapies

Frontiers in Oncology, 2022
Extracellular DNA may serve as marker in liquid biopsies to determine individual diagnosis and prognosis in cancer patients. Cell death or active release from various cell types, including immune cells can result in the release of DNA into the ...
Medina Mamtimin, Medina Mamtimin, Akif Pinarci, Chao Han, Chao Han, Attila Braun, Hans-Joachim Anders, Hans-Joachim Anders, Thomas Gudermann, Thomas Gudermann, Elmina Mammadova-Bach, Elmina Mammadova-Bach   +11 more
doaj   +1 more source

Campylobacter jejuni biofilms contain extracellular DNA and are sensitive to DNase I treatment

Frontiers in Microbiology, 2015
Biofilms make an important contribution to survival and transmission of bacterial pathogens in the food chain. The human pathogen Campylobacter jejuni is known to form biofilms in vitro in food chain-relevant conditions, but the exact roles and ...
Helen L Brown, Helen L Brown, Kate eHanman, Mark eReuter, Roy P Betts, Arnoud H M Van Vliet   +5 more
doaj   +1 more source

Extracellular DNA: A Critical Aspect of Marine Biofilms

Microorganisms, 2022
Multispecies biofilms represent a pervasive threat to marine-based industry, resulting in USD billions in annual losses through biofouling and microbiologically influenced corrosion (MIC). Biocides, the primary line of defence against marine biofilms, now face efficacy and toxicity challenges as chemical tolerance by microorganisms increases. A lack of
Benjamin Tuck, Silvia J. Salgar-Chaparro, Elizabeth Watkin, Anthony Somers, Maria Forsyth, Laura L. Machuca   +5 more
openaire   +3 more sources

Function of Surface-Associated Protein and DNA on Extracellular Vesicles [PDF]

, 2018
Extracellular vesicles (EVs), including exosomes, are nano-sized, lipid bilayer enclosed vesicles that are released into the extracellular environment from by almost all cells.
Shelke, Ganesh
core   +1 more source