Results 61 to 70 of about 2,813,323 (292)

Indirect DNA extraction method suitable for acidic soil with high clay content

MethodsX, 2018
DNA extraction is an essential procedure when investigating microbial communities in environmental samples by sequencing technologies. High clay soils can be problematic as DNA adsorbs to the clay particles and can thereby be preserved from lysed, non ...
Eva Högfors-Rönnholm, Stephan Christel, Sten Engblom, Mark Dopson   +3 more
doaj   +1 more source

Genomic insights into diverse bacterial taxa that degrade extracellular DNA in marine sediments [PDF]

, 2021
Extracellular DNA is a major macromolecule in global element cycles, and is a particularly crucial phosphorus, nitrogen and carbon source for microorganisms in the seafloor. Nevertheless, the identities, ecophysiology and genetic features of DNA-foraging
Hofmann, Thilo, Hubert, Casey R.J., Schintlmeister, Arno, Watzka, Margarete, Noel, Amy, Pelikan, Claus, Richter, Andreas, Rattei, Thomas, Herbold, Craig W., Bhatnagar, Srijak, Wasmund, Kenneth, Wagner, Michael, Hausmann, Bela, Loy, Alexander, Thomas Rattei, Casey R. J. Hubert, Kenneth Wasmund, Amy Noel, Craig W. Herbold, Michael Wagner, Thilo Hofmann, Srijak Bhatnagar, Arno Schintlmeister, Margarete Watzka, Bela Hausmann, Andreas Richter, Alexander Loy, Claus Pelikan   +27 more
core   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

A concept of natural genome reconstruction. Part 2. Effect of extracellular double-stranded DNA fragments on hematopoietic stem cells

Вавиловский журнал генетики и селекции
In this part of the study, the first component of the concept of “natural genome reconstruction” is being proven. It was shown with mouse and human model organisms that CD34+ hematopoietic bone marrow progenitors take up fragments of extracellular double-
V. S. Ruzanova, S. G. Oshikhmina, A. S. Proskurina, G. S. Ritter, S. S. Kirikovich, E. V. Levites, Y. R. Efremov, T. V. Karamysheva, M. I. Meschaninova, A. L. Mamaev, O. S. Taranov, A. S. Bogachev, S. V. Sidorov, S. D. Nikonov, O. Y. Leplina, A. A. Ostanin, E. R. Chernykh, N. A. Kolchanov, E. V. Dolgova, S. S. Bogachev   +19 more
doaj   +1 more source

Bioactive DNA from extracellular vesicles and particles [PDF]

Cell Death & Disease, 2020
Abstract Extracellular vesicles (EVs) and particles (EPs) have recently emerged as active carriers of molecular biomarkers and mediators of intercellular communication. While most investigations have focused exclusively on the protein, lipid and RNA constituents of these extracellular entities, EV/EP DNA remains poorly understood, despite DNA
Ethan Z. Malkin, Scott V. Bratman
openaire   +2 more sources

DNA-Driven Photothermal Amplification Transducer for Highly Sensitive Visual Determination of Extracellular Vesicles

, 2023
Extracellular vesicles (EVs) are crucial focus of current biomedical research and future medical diagnosis. However, the requirement for specialized sophisticated instruments for quantitative readouts has limited the sensitive measurement of EVs to ...
Fei Han (24308), Ziling Ding (14298467), Zhangrun Xu, Zhangrun Xu (276306), Xu Zhang (49484), Xu Zhang, Yunyun Wei (9570009), Fei Han, Yunyun Wei, Ziling Ding   +9 more
core   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

The microbiome in human skin aging

FEBS Letters, EarlyView.
Age‐related skin changes encompass the well‐known visible phenotypic alterations, together with microbiome dysbiosis and a series of molecular aging hallmarks. These hallmarks characterize not only a fully stablished aged phenotype but also the skin aging process itself.
Manuel Huerta Arana, Cornelia Wiegand, Omid Omrani, Mario Fabri   +3 more
wiley   +1 more source

Consideration of dornase alfa for the treatment of severe COVID-19 acute respiratory distress syndrome

New Microbes and New Infections, 2020
We propose a likely contribution to severe COVID-19 morbidity by extracellular DNA in neutrophil extracellular traps (NETs). Dornase alfa degrades extracellular DNA to reduce mucus rigidity and accumulation, and was associated with respiratory ...
A.P. Earhart, Z.M. Holliday, H.V. Hofmann, A.G. Schrum   +3 more
doaj   +1 more source