Results 101 to 110 of about 673,761 (305)

The role of the phosphopantetheinyltransferase enzyme, PswP, in the biosynthesis of antimicrobial secondary metabolites by Serratia marcescens Db10 [PDF]

, 2014
Phosphopantetheinyl transferase (PPTase) enzymes fulfil essential roles in primary and secondary metabolism in prokaryotes, archaea and eukaryotes. PPTase enzymes catalyse the essential modification of the carrier protein domain of fatty acid synthases ...
Gerc, Amy J., Stanley-Wall, Nicola R.; id_orcid, Coulthurst, Sarah J.; id_orcid   +2 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Fungal Biodegradation of Procyanidin in Submerged Fermentation

Fermentation
The coffee industry generates a large amount of waste that is difficult to treat due to its chemical composition, namely, the presence of caffeine and its derivatives, as well as recalcitrant molecules such as tannins (mainly condensed tannins or ...
Leidy Johana Valencia-Hernández, Jorge E. Wong-Paz, J. Alberto Ascacio-Valdés, Arely Prado-Barragan, Mónica L. Chávez-González, Cristóbal N. Aguilar   +5 more
doaj   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Microbial Metabolic Limitations and Their Relationships with Sediment Organic Carbon Across Lake Salinity Gradient in Tibetan Plateau

Microorganisms
Inland lakes, contributing substantially to the global storage of sediment organic carbon (SOC), are subject to marked changes in salinity due to climate warming.
Weizhen Zhang, Jianjun Wang, Yun Li, Chao Song, Yongqiang Zhou, Xianqiang Meng, Ruirui Chen   +6 more
doaj   +1 more source

Extracellular Enzymes Produced by Marine Eukaryotes, Thraustochytrids

Bioscience, Biotechnology, and Biochemistry, 2009
Extracellular enzymes produced by six strains of thraustochytrids, Thraustochytrium, Schizochytrium, and Aurantiochytrium, were investigated. These strains produced 5 to 8 kinds of the extracellular enzymes, depending on the species. Only the genus Thraustochytrium produced amylase.
TAOKA, Yousuke, NAGANO, Naoki, OKITA, Yuji, IZUMIDA, Hitoshi, SUGIMOTO, Shinichi, HAYASHI, Masahiro   +5 more
openaire   +2 more sources

Extracellular enzyme activities and enzyme ratios in different horizons in arctic soils.

, 2014
Left panel: Extracellular enzyme activities for the C-acquiring enzyme cellobiohydrolase (CBH), the N-acquiring enzyme leucine-amino-peptidase (LAP) and the oxidative enzyme phenoloxidase (POX).
Anna Knoltsch (547468), Tim Urich (49469), Andreas Richter (87142), Sandra Kienzl (547467), Jörg Schnecker (547460), Birgit Wild (305428), Florian Hofhansl (517079), Mounir Takriti (547472), Robert Mikutta (547470), Norman Gentsch (547464), Ricardo J. Eloy Alves (547461), Lucia Fuchslueger (547463), Olga Shibistova (305425), Hana Šantrůčková (547471), Nikolay Lashchinskiy (547469), Georg Weltin (547473), Georg Guggenberger (305432), Jiří Bárta (547462), Petr Čapek (2669128), Antje Gittel (547465), Angelika Hofer (547466)   +20 more
core   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Irrigation frequency and irrigation amount of micro-sprinkler irrigation mulched regulate N2O emission of tomato (Solanum lycopersicum) soil

Frontiers in Sustainable Food Systems
IntroductionThere is a limited amount of research available on how changes in soil hydrothermal cycles impact soil N2O emissions in greenhouses that use a tomato irrigation system with micro-sprinkler irrigation mulched (MSM).MethodsThis study examined ...
Haijian Yang, Mingzhi Zhang, Wenqian Wu, Na Xiao, Mengmeng Sun, Yuan Li   +5 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source