Results 41 to 50 of about 344,575 (257)

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Cell density–dependent nuclear‐cytoplasmic shuttling of SETDB1 integrates with Hippo signaling to regulate YAP1‐mediated transcription

FEBS Letters, EarlyView.
At low cell density, SETDB1 and YAP1 accumulate in the nucleus. As cell density increases, the Hippo pathway is gradually activated, and SETDB1 is associated with increased YAP1 phosphorylation. At high cell density, phosphorylated YAP1 is sequestered in the cytoplasm, while SETDB1 becomes polyubiquitinated and degraded by the ubiquitin–proteasome ...
Jaemin Eom, Jaewoong Jang, Jung Sun Park, Yong‐Kook Kang   +3 more
wiley   +1 more source

ALS-like pathology diminishes swelling of spinal astrocytes in the SOD1 animal model

Frontiers in Cellular Neuroscience
Astrocytes are crucial for the functioning of the nervous system as they maintain the ion homeostasis via volume regulation. Pathological states, such as amyotrophic lateral sclerosis (ALS), affect astrocytes and might even cause a loss of such functions.
Tereza Filipi, Tereza Filipi, Jana Tureckova, Ondrej Vanatko, Ondrej Vanatko, Martina Chmelova, Monika Kubiskova, Monika Kubiskova, Natalia Sirotova, Natalia Sirotova, Stanislava Matejkova, Lydia Vargova, Miroslava Anderova   +12 more
doaj   +1 more source

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source

A Space‐Time Conversion Vehicle for Programmed Multi‐Drugs Delivery into Pancreatic Tumor to Overcome Matrix and Reflux Barriers

Advanced Science, 2022
The numerous biological barriers, which limit pharmacotherapy of pancreatic carcinoma, including inadequate drug accumulation in the tumor environment, a dense extracellular matrix (ECM) and efficient drug‐efflux mechanisms, illustrate the requirement of
Taotao Huo, Xiaoyi Zhang, Min Qian, Huifang Nie, Dong Liang, Chenteng Lin, Yafeng Yang, Wei Guo, Ulrich Lächelt, Rongqin Huang   +9 more
doaj   +1 more source

Adaptaquin is selectively toxic to glioma stem cells through disruption of iron and cholesterol metabolism

Molecular Oncology, EarlyView.
Adaptaquin selectively kills glioma stem cells while sparing differentiated brain cells. Transcriptomic and proteomic analyses show Adaptaquin disrupts iron and cholesterol homeostasis, with iron chelation amplifying cytotoxicity via cholesterol depletion, mitochondrial dysfunction, and elevated reactive oxygen species.
Adrien M. Vaquié, Davod R. Shah, Eliane E. S. Brechbühl, Michael McNicholas, Zhaoyang Xu, John H. Stockley, Laura Morcom, Diana Gold Diaz, Gemma C. Girdler, Rachel V. Seear, Gabriel Balmus, Rajiv R. Ratan, Harry Bulstrode, James A. Nathan, Manav Pathania, Kevin M. Brindle, David H. Rowitch   +16 more
wiley   +1 more source

A three-dimensional mathematical model for the signal propagation on a neuron’s membrane.

Frontiers in Computational Neuroscience, 2015
In order to be able to examine the extracellular potential's influence on network activity and to better understand dipole properties of the extracellular potential, we present and analyze a three-dimensional formulation of the cable equation which ...
Konstantinos eXylouris, Gabriel eWittum
doaj   +1 more source

The neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy

Molecular Oncology, EarlyView.
ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.
Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal   +8 more
wiley   +1 more source

Dissecting the Simultaneous Extracellular/Intracellular Contributions to Cr(VI) Reduction under Aerobic and Anaerobic Conditions Using the Newly Isolating Cr(VI)-Reducing Bacterium of Pseudomonas sp. HGB10

Microorganisms
Quantifying extracellular and intracellular contributions to Cr(VI) reduction is crucial for understanding bacterial Cr(VI)-reduction mechanisms. However, this contribution under different oxygen conditions remains largely unexplored.
Shenglei Chen, Xiaoyu Wang, Qinyi Zhao, Qiao Xu, Yini Zhang   +4 more
doaj   +1 more source

Epithelial sodium channels (ENaC) produce extracellular positive DC potentials in the retinal neuroepithelium

Data in Brief, 2016
Positive DC (direct current) potentials were measured in the extracellular space in the retinal neuroepithelium of chick embryos. The positive DC potential was suppressed by amiloride, a blocker for epithelial Na+ channels (ENaC).
Masayuki Yamashita
doaj   +1 more source