Results 21 to 30 of about 389,852 (268)

Generation of skeletal muscle cells from pluripotent stem cells: advances and challenges

Frontiers in Cell and Developmental Biology, 2015
Human pluripotent stem cells (hPSCs) possess unlimited proliferative potential while maintaining the ability to differentiate into any cell type including skeletal muscle cells (SMCs). hPSCs are amenable to genetic editing and can be derived from patient
Ramzey eAbujarour, Bahram eValamehr
doaj   +1 more source

Dissecting regulatory pathways for transcription recovery following DNA damage reveals a non-canonical function of the histone chaperone HIRA

Nature Communications, 2021
How gene expression reactivation is promoted following DNA damage is not yet clear. Here, the authors identify a role for the human histone chaperone complex HIRA in transcription restart following UV damage independently of its function in new H3.3 ...
Déborah Bouvier, Juliette Ferrand, Odile Chevallier, Michelle T. Paulsen, Mats Ljungman, Sophie E. Polo   +5 more
doaj   +1 more source

Single-cell Visualization of Chromosome Transcriptional Territories by RNA-paint

Bio-Protocol, 2016
We developed a FISH-based method to directly assess chromosome-wide transcriptional activity, thereby enabling the visualization of the actively transcribed fraction of a chromosome at the single-cell level.
Céline Vallot, Claire Rougeulle
doaj   +1 more source

A primate-specific retroviral enhancer wires the XACT lncRNA into the core pluripotency network in humans

Nature Communications, 2019
XACT is a primate-specific TE-derived lncRNA that coats active X chromosomes in pluripotent cells and may contribute to species-specific regulation of X chromosome inactivation.
Miguel Casanova, Madeleine Moscatelli, Louis Édouard Chauvière, Christophe Huret, Julia Samson, Tharvesh Moideen Liyakat Ali, Olga Rosspopoff, Claire Rougeulle   +7 more
doaj   +1 more source

Imaging the response to DNA damage in heterochromatin domains reveals core principles of heterochromatin maintenance

Nature Communications, 2021
The heterochromatin compartment is key for genome stability and cell fate transitions. Here, by tracking the heterochromatin response to UV damage in real time, the authors reveal how heterochromatin features are preserved following a genotoxic stress ...
Anna Fortuny, Audrey Chansard, Pierre Caron, Odile Chevallier, Olivier Leroy, Olivier Renaud, Sophie E. Polo   +6 more
doaj   +1 more source

Deciphering tissue structure and function using spatial transcriptomics

Communications Biology, 2022
Walker et al. review methods used to analyse spatial transcriptomics data and discuss open questions and areas of future development.
Benjamin L. Walker, Zixuan Cang, Honglei Ren, Eric Bourgain-Chang, Qing Nie   +4 more
doaj   +1 more source

LABOR EMPLOYMENT OPPORTUNITIES IN COFFEE PRODUCTION IN SOUTHERN LAO PEOPLE’S DEMOCRATIC REPUBLIC

Journal of Asian Rural Studies, 2018
General debate on the issue of labor employment is related to employment opportunity, pattern of labor employment, the contribution to labor employment, and the impact of labor employment on livelihoods. This paper examines labor employment opportunities
Dexanourath Seneduangdeth, Kiengkay Ounmany, Saithong Phommavong, Kabmanivanh Phouxay, Keophouthon Hathalong   +4 more
doaj   +1 more source

iPSC-derived trimodal T cells engineered with CAR, TCR, and hnCD16 modalities can overcome antigen escape in heterogeneous tumors

Cell Reports Medicine
Summary: Although chimeric antigen receptor (CAR) T cells have demonstrated therapeutic activity in hematopoietic malignancies, tumor heterogeneity has impeded the efficacy of CAR T cells and their extension into successful solid tumor treatment.
Bi-Huei Yang, Alma Gutierrez, Angela Liao, Soheila Shirinbak, Bjoern Gaertner, Mochtar Pribadi, Hui-Yi Chu, Pei-Fang Tsai, Yu-Sheng Eason Lin, David Gonzalez, Wen-I Yeh, Chia-Wei Chang, Ryan Bjordahl, Tom Lee, Martin Hosking, Eigen Peralta, Bahram Valamehr   +16 more
doaj   +1 more source

The fate of pentagastrin [PDF]

Gut, 1971
Pentagastrin is rapidly removed from the circulation of the dog. It is excreted by the liver into the bile, largely in an inactivated form. Preliminary results indicate that it meets the same fate in man.
B H, Stagg, J M, Temperley, J H, Wyllie
openaire   +2 more sources

A chemical approach facilitates CRISPRa-only human iPSC generation and minimizes the number of targeted loci required

Future Science OA
Aim: We explored the generation of human induced pluripotent stem cells (iPSCs) solely through the transcriptional activation of endogenous genes by CRISPR activation (CRISPRa). Methods: Minimal number of human-specific guide RNAs targeting a limited set
Ramzey Abujarour, Jason Dinella, Mochtar Pribadi, Lauren K Fong, Matthew Denholtz, Alma Gutierrez, Matt Haynes, Enaaya Mahmood, Tom T Lee, Sheng Ding, Bahram Valamehr   +10 more
doaj   +1 more source