Advancing diffusion-driven small-scale fed-batch technologies
The fed-batch cultivation mode is the most prevalent in industrial bioprocesses, as it effectively mitigates a multitude of detrimental phenomena, including overflow metabolism, mixed acid formation, and oxygen limitation.
Lüchtrath, Clara Agnes
core +1 more source
Chlorella vulgaris biorefineries: sustainable biofuels and high‐value carbon capture
Biofuels, Bioproducts and Biorefining, EarlyView.Abstract Global reliance on fossil fuels has created urgent economic and environmental challenges, yet large‐scale use of algal biomass remains limited by production costs. Industrial scaling is constrained by inefficient harvesting and the technical challenges of processing recalcitrant cell walls.
Sandyelle Ferreira Alcântara Araújo +13 more
wiley +1 more source
Efficient biosynthesis of 2-keto-D-gluconic acid by fed-batch culture of metabolically engineered Gluconobacter japonicus. [PDF]
Synth Syst Biotechnol, 2019 Zeng W +5 more
europepmc +1 more source
Harnessing plant‐based platform for low‐cost cellulosic sugar recovery from bioenergy crops
Biofuels, Bioproducts and Biorefining, EarlyView.Abstract The price of pure cellulase enzyme for the recovery of fermentable cellulosic sugars is one of the major challenges that limit the commercialization of second‐generation biofuels and bio‐based products. This work shows a means to greatly reduce the cost of cellulases. The abundant capacity of plants to synthesize and hyperaccumulate transgenic
Shraddha Maitra +4 more
wiley +1 more source
Affinity Peptides With pH Sensitivity for the Enrichment of CD38+ Cells
Biotechnology and Bioengineering, EarlyView.ABSTRACT The selective enrichment of cell populations based on surface markers is critical for the advancement of gene and cell therapies. Current antibody‐based cell isolation methods, such as fluorescence‐ and magnetic‐activated cell sorting (FACS and MACS), offer high specificity but are limited by scalability, cost, and potential adverse effects on
Gabrielle Rusch +7 more
wiley +1 more source
Zymobacter palmae pyruvate decarboxylase production process development: Cloning in Escherichia coli, fed-batch culture and purification. [PDF]
Eng Life Sci, 2019 Alcover N +3 more
europepmc +1 more source
Multi‐Scale Hybrid Modeling to Predict Cell Culture Process With Metabolic Phase Transitions
Biotechnology and Bioengineering, EarlyView.ABSTRACT To advance understanding of cellular metabolism and reduce batch‐to‐batch variability in cell culture processes, this study introduces a multi‐scale hybrid modeling framework designed to simulate and predict the dynamic behavior of CHO cell cultures undergoing metabolic phase transitions.
Keqi Wang, Sarah W. Harcum, Wei Xie
wiley +1 more source
Incorporating shaken 24-deep-well plate fed-batch culture shortens CHO cell line development time. [PDF]
CytotechnologyOhira S, Omasa T.
europepmc +1 more source
On the Prediction of pDNA Productivity Across Diverse Bioprocesses Using Ensemble Hybrid Models
Biotechnology and Bioengineering, EarlyView.ABSTRACT The global demand for plasmid DNA (pDNA) is rapidly increasing due to its key role in the manufacture of mRNA vaccines and advanced therapy medicinal products (ATMPs). However, the reliance on suboptimal batch and/or fed‐batch processes, as well as the diversity and increasing complexity of plasmid constructs pose significant challenges during
Nikolaos Stratis +2 more
wiley +1 more source
Enhancing monoclonal antibody production efficiency using CHO-MK cells and specific media in a conventional fed-batch culture. [PDF]
CytotechnologySaeki H, Fueki K, Maeda N.
europepmc +1 more source