Results 181 to 190 of about 28,581 (261)

Status and future of recombinant adeno‐associated virus vector manufacturing

Biotechnology Progress, EarlyView.
Abstract Sixty years of adeno‐associated virus (AAV) research illustrates a trajectory marked by basic science exploration, iterative innovation, persistent challenges, a number of clinical setbacks, as well as commercial therapeutic triumphs. This continual evolution has led to recombinant AAV (rAAV) becoming a cornerstone of modern gene therapy ...
Frank Agbogbo, David Dismuke
wiley   +1 more source

Scaled up fed-batch production of recombinant alpha-1-antitrypsin by CHO cells in single-use surface aerated orbital shaken bioreactor. [PDF]

Sci Rep
Tang WQ, Jiang CQZ, Zheng ZY, Lau A, Bi X, Zhang W, Ng SK.   +6 more
europepmc   +1 more source

Advanced glucose control strategies leveraging Raman spectroscopy for optimized mammalian cell culture manufacturing

Biotechnology Progress, EarlyView.
Abstract Maintaining consistent quality in the manufacturing of biotherapeutic proteins in mammalian cell culture is challenging, with unplanned deviations causing inconsistencies and potential batch failure. Current methods for monitoring and controlling critical process parameters (CPPs) rely on slow, labor‐intensive offline analyses.
Matthew Banner, Daniel Ray, Irfan Ahanger, Fabio Zurlo, Noah Hitchcock, Rajesh Mistry, Kasia Kozakowska‐McDonnell, Matthew Cheeks, Richard Turner, Jonathan Welsh, Suzanne S. Farid, Michael Thomas, Stephen Goldrick   +12 more
wiley   +1 more source

Optimization of cultivation strategies for production of recombinant human papillomavirus type 58 major capsid protein L1 in Hansenula polymorpha. [PDF]

Bioresour Bioprocess
Kopitak N, Phimsen W, Sripui K, Khunsom A, Pongsuwichedsak N, Boontawon T, Theeranan T, Boonchird C, Pongtharangkul T.   +8 more
europepmc   +1 more source

Scalable catalyst production process for oleate hydratase whole‐cell biocatalysis

Biotechnology Progress, EarlyView.
This study establishes a scalable lactose‐induced fed‐batch fermentation for an Escherichia coli whole‐cell biocatalyst containing an oleate hydratase. Operation at a higher growth rate markedly increased biomass yields while maintaining catalyst activity and was demonstrated at 150 L scale.
Rebekka Horstmann, Mario Beckers, Jörn Viell, Fabian Allan Watt, Valentin Steier, Marcel Mann, Jørgen B. Magnus   +6 more
wiley   +1 more source

Engineered Bacillus subtilis for high-yield nattokinase production and therapeutic potential in obesity management. [PDF]

J Anim Sci Biotechnol
Liu Y, Zhu L, Yao T, Wang Y, Xie C, Gao L.   +5 more
europepmc   +1 more source

Application of commercial ultrafiltration membranes for protein purification by high performance countercurrent membrane purification (HPCMP)

Biotechnology Progress, EarlyView.
Abstract High performance countercurrent membrane purification (HPCMP) has emerged as a promising diffusive separation strategy for the purification of recombinant proteins and monoclonal antibodies (mAbs). However, prior studies employed dialysis membranes, which are designed for diffusive separations but have not been validated for biopharmaceutical ...
Aylin Mohammadzadehmarandi, Nate Heller, Mohammadreza Ahmadimashhadi, Jon Petrone, Nicholas Marchand, Siavash Darvishmanesh, Ka Zhang, Stefano Menegatti, Andrew L. Zydney   +8 more
wiley   +1 more source

Microbial Oil Production by <i>Yarrowia lipolytica</i> Under Semi-Continuous Cultivation and Potential Utility of Spent Supernatant. [PDF]

Foods
Uğur Ş, Zieniuk B, Górnicka M, Nowak D, Fabiszewska A.   +4 more
europepmc   +1 more source

Screening of bispecific antibodies during single cell cloning of recombinant Chinese hamster ovary cell lines to improve titer and product quality

Biotechnology Progress, EarlyView.
Abstract Development of therapeutic bispecific antibodies (BsAbs) poses significant manufacturability challenges associated with correct chain pairing and an increased diversity of impurities. Here we address this with a product quality assessment during cell line development (CLD), forgoing the need for genetic characterization during cell line ...
Molly Robinson, Jessica Willmott, Holly Corrigall, Daniela Lega, Joseph Stirling, Vithiya Vimalraj, Mitul Patel, Robyn Emmins   +7 more
wiley   +1 more source

A Hybrid Modeling Framework for Predictive Digital Twins of CHO Cell Culture. [PDF]

Comput Struct Biotechnol J
Richelle A, Andersson D, Antonakoudis A, Jakobsson J, Pijeaud S, Vernersson A, Trygg J.   +6 more
europepmc   +1 more source