Results 41 to 50 of about 4,571,974 (249)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Effect of alfalfa supplementary change dietary non-fibrous carbohydrate (NFC) to neutral detergent fiber (NDF) ratio on rumen fermentation and microbial function in Gansu alpine fine wool sheep (Ovis aries)

open access: yesAnimal Biotechnology
Bodyweight loss and rumen microbial dysfunction of grazing sheep was a challenge for the sheep production industry during cold season, which were considered to correlated with under-roughage-feeding. Alfalfa is a good roughage supplementary for ruminants,
Qian Chen   +7 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Evaluating the effects of supplementing Saccharomyces cerevisiae in the feed or drinking water on stress susceptibility of broilers

open access: yesJournal of Applied Poultry Research, 2022
SUMMARY: Poultry are susceptible to stress as a response to a multitude of environmental stressors such as handling, vaccination, feed outages, rearing on used litter, and elevated ambient temperature.
E.B. Sobotik   +3 more
doaj   +1 more source

Interventions to achieve long-term weight loss in obese older people [PDF]

open access: yes, 2010
This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Age and Ageing following peer review. The definitive publisher-authenticated version Witham, M. & Avenell, A. (2010).
Witham, Miles D.   +2 more
core   +1 more source

Adaptive Foam 3D Printing of Ultralight and Multifunctional Materials

open access: yesAdvanced Engineering Materials, EarlyView.
Adaptive foam 3D printing, enabled by expandable microspheres, imparts cellular structures to thermoplastic and thermosetting polymers, manufactured through a variety of processes including fused filament fabrication, direct ink writing, digital light processing, and inkjet printing.
Nariman Rajabifar, Amir Ameli
wiley   +1 more source

Enhancing post-harvest quality of Red Globe grapes using fermented cheese whey edible coatings

open access: yesInternational Journal of Food Properties
Red Globe grapes (Vitis vinifera) are widely consumed for their taste and bioactive compounds but are highly perishable during post-harvest handling. This study evaluated the effectiveness of an edible coating formulated from cheese whey fermented by ...
Nidhar Luthfiyah Abdurodjak   +4 more
doaj   +1 more source

Ethanol Production from Waste Potato Mash by Using Saccharomyces Cerevisiae

open access: yesApplied Sciences, 2012
Bio-ethanol is one of the energy sources that can be produced by renewable sources. Waste potato mash was chosen as a renewable carbon source for ethanol fermentation because it is relatively inexpensive compared with other feedstock considered as food ...
Gulten Izmirlioglu, Ali Demirci
doaj   +1 more source

Effect of long-term consumption of <i>Lacticaseibacillus paracasei</i> strain Shirota-fermented milk on weight loss in the institutionalized oldest old: an exploratory study

open access: yesBioscience of Microbiota, Food and Health
Older individuals with care needs and lower body mass indices (BMIs) are more likely to be malnourished. Unintentional weight loss, an indicator of malnutrition, significantly impacts the physical function and poor prognosis of older adults. This study aimed to explore the effects of long-term consumption of Lacticaseibacillus paracasei strain Shirota ...
NAITO, Eiichiro   +9 more
openaire   +2 more sources

FastNano Liquid: An Automated Platform for Small‐Angle X‐ray Scattering‐Based Materials Discovery

open access: yesAdvanced Engineering Materials, EarlyView.
We present FastNano Liquid, an automated small‐ and wide‐angle X‐ray scattering platform for the combined synthesis and characterization of (nano)materials. The platform is coupled to varied reactor workflows for both in situ studies of reaction kinetics and ex situ screening of synthesis conditions to support machine learning‐guided exploration ...
Pierre‐Baptiste Flandrin   +16 more
wiley   +1 more source

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