Results 201 to 210 of about 10,827 (244)

Resolution and reconstitution of spinach ferredoxin-NADP+ reductase

Biochemical and Biophysical Research Communications, 1978
Abstract The apoprotein from spinach ferredoxin-NADP + reductase was prepared by treatment with 3 M calcium chloride. This procedure caused complete removal of the FAD prosthetic group together with considerable denaturation of the apoprotein. Thus, the recovery of total activity upon reconstitution with FAD was only 30%.
Peter Böger, A. San Pietro, G. Bookjans
openaire   +3 more sources

Immobilized Ferredoxin-NADP+ Reductase: Preparation and Properties

The Journal of Biochemistry, 1982
Immobilized ferredoxin-NADP+ reductase (FNR) was prepared by coupling reaction of CNBr-Sepharose 4B with the spinach enzyme. The immobilized FNR was found to retain the activity of complex formation with ferredoxin as well as the enzymatic activities such as NADPH-diaphorase and NADPH-cytochrome c reductase activities.
Nobuko Sugimoto, Masateru Shin, Naoko Sakihama, Tomoaki Miyazawa, Reiko Oshino   +4 more
openaire   +3 more sources

Atomic structure of ferredoxin-NADP+ reductase: prototype for a structurally novel flavoenzyme family.

Science, 1991
The three-dimensional structure of spinach ferredoxin-NADP+ reductase (NADP+, nicotinamide adenine dinucleotide phosphate) has been determined by x-ray diffraction at 2.6 angstroms (A) resolution and initially refined to an R factor of 0.226 at 2.2 A ...
P. Karplus, MJ Daniels, Herriott
semanticscholar   +1 more source

Studies on the holoenzyme biogenesis of the spinach ferredoxin-NADP+ reductase

Archives of Biochemistry and Biophysics, 1992
An expression plasmid, pPreFNR, in which the DNA sequence coding for the spinach ferredoxin-NADP+ reductase precursor was under the control of prokaryotic transcription and translation initiation signals has been constructed. The plasmid directed the synthesis in Escherichia coli of a 43-kDa immunoreactive polypeptide which could be identified with the
A. Aliverti, L. Ferretti, G. Zanetti
openaire   +3 more sources

PARTICIPATION OF FERREDOXIN-NADP-REDUCTASE AND ATP-SYNTHASE IN THE FORMATION OF THE PROTECTIVE RESPONSE OF POTATO TO DROUGHT

Перспективные прикладные исследования и инновации: сборник статей международной научной конференции (Кингисепп, Май 2023), 2023
При воздействии на растения стрессовых факторов окружающей среды, уровень генерации активных форм кислорода в растительных клетках увеличивается. При комбинированном стрессе (вирусных инфекциях, засухе, температурном стрессе, загрязнении тяжелыми ...
Ляйсан Салаватовна Муртазина, Вячеслав Олегович Цветков   +1 more
semanticscholar   +1 more source

Crystals of Anabaena PCC 7119 ferredoxin-NADP+ reductase

Journal of Molecular Biology, 1991
Crystals of ferredoxin-NADP+ reductase (FNR) from the cyanobacterium Anabaena PCC 7119 are grown in the presence of polyethylene glycol 6000 and beta-octyl glucoside. They belong to the hexagonal system. The cell parameters are a = b = 87.8 A, c = 92.7 A, space group P6(1) or P6(5), and a Vm of 3.0 A3/dalton for one molecule of 36,000 daltons per ...
Juan C. Fontecilla-Camps, Milagros Medina, Carlos Gómez-Moreno, Michel Frey, Laurence Serre   +4 more
openaire   +3 more sources

The extremophilic Andean isolate Acinetobacter sp. Ver3 expresses two ferredoxin‐NADP+ reductase isoforms with different catalytic properties

FEBS Letters
Ferredoxin/flavodoxin‐NADPH reductases (FPRs) catalyze the reversible electron transfer between NADPH and ferredoxin/flavodoxin. The Acinetobacter sp.
Alejandro Palavecino, M. G. Sartorio, Néstor Carrillo, N. Cortez, A. Bortolotti   +4 more
semanticscholar   +1 more source

Chemical modification of the active site of ferredoxin-NADP+ reductase and conformation of the binary ferredoxin/ferredoxin-NADP+ reductase complex in solution

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1988
Abstract Ferredoxin-NADP+ reductase (EC 1.18.1.2) was chemically modified by the triplet probe eosin isothiocyanate (eosin-NES). Incorporation of 1 mol eosin-NCS/mol ferredoxin-NADP+ reductase completely inhibited binding of NADP+/NADPH to the enzyme.
Enno C. Apley, Richard Wagner
openaire   +2 more sources

Amino acid residues responsible for the different pH dependency of cell-specific ferredoxins in the electron transfer reaction with ferredoxin-NADP+ reductase from maize leaves.

Journal of Biochemistry (Tokyo)
In the chloroplast stroma, dynamic pH changes occur from acidic to alkaline in response to fluctuating light conditions. We investigated the pH dependency of the electron transfer reaction of ferredoxin-NADP+ reductase (FNR) with ferredoxin (Fd ...
Y. Kimata‐Ariga, Hikaru Tanaka, Shunsuke Kuwano   +2 more
semanticscholar   +1 more source

High temperature triggers the metabolism of S-nitrosothiols in sunflower mediating a process of nitrosative stress which provokes the inhibition of ferredoxin-NADP reductase by tyrosine nitration.

Plant, Cell and Environment, 2011
High temperature (HT) is considered a major abiotic stress that negatively affects both vegetative and reproductive growth. Whereas the metabolism of reactive oxygen species (ROS) is well established under HT, less is known about the metabolism of ...
Mounira Chaki, R. Valderrama, A. Fernández-Ocaña, A. Carreras, M. V. Gómez-Rodríguez, J. López-Jaramillo, J. Begara-Morales, Beatriz Sánchez-Calvo, F. Luque, M. Leterrier, F. J. Corpas, J. B. Barroso   +11 more
semanticscholar   +1 more source