Results 171 to 180 of about 110,840 (210)

Protoplast Transformation of Filamentous Fungi

2010
The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.
B Gillian, Turgeon, Bradford, Condon, Jinyuan, Liu, Ning, Zhang   +3 more
openaire   +2 more sources

Genetic Engineering of Filamentous Fungi

Science, 1989
Filamentous fungi are important in medicine, industry, agriculture, and basic biological research. For example, some fungal species are pathogenic to humans, whereas others produce β-lactam antibiotics (penicillin and cephalosporin). Industrial strains produce large amounts of enzymes, such as glucosamylose and proteases, and low molecular weight ...
W E, Timberlake, M A, Marshall
openaire   +2 more sources

Transposable Elements in Filamentous Fungi

Annual Review of Microbiology, 2003
▪ Abstract  The past 10 years have been productive in the characterization of fungal transposable elements (TEs). All eukaryotic TEs described are found including an extraordinary prevalence of active members of the pogo family. The role of TEs in mutation and genome organization is well documented, leading to significant advances in our perception of
Marie-Josée, Daboussi, Pierre, Capy
openaire   +2 more sources

Flow Cytometry for Filamentous Fungi

2020
Flow cytometry is a powerful high-throughput method, which enables a fast and multi-parameter analysis of single cells and particles. A plethora of different dyes for flow cytometry are available to label different parts of a cell in addition to in vivo markers like fluorescent proteins.
openaire   +2 more sources

MATING TYPE IN FILAMENTOUS FUNGI

Annual Review of Genetics, 1997
▪ Abstract  Mating type genes regulate sexual compatibility and sexual reproduction in fungi. This review focuses on recent molecular analyses of well-characterized mating systems from representative ascomycete (Neurospora crassa, Podospora anserina) and basidiomycete (Ustilago maydis, Coprinus cinereus, Schizophyllum commune) fungi.
J W, Kronstad, C, Staben
openaire   +2 more sources

Chromosome visualisation in filamentous fungi

Journal of Microbiological Methods, 2006
Many attempts have been made to study the chromosomes of fungi, but a major problem is that fungal nuclei are so small. Fungal chromosomes are at the lowest resolution of light microscopy; thus few attempts to visualise fungal chromosomes have been successful. Fungi examined have been mainly Ascomycotina.
openaire   +2 more sources

Otomycosis due to Filamentous Fungi

Mycopathologia, 2011
Otomycosis is common throughout the world but barely studied in Spain. Our objective was to determine the microbiological and epidemiological characteristics of this pathology in Cadiz (Spain) between 2005 and 2010. Samples from patients with suspicion of otomycosis underwent a direct microscopic examination and culture on different media for fungi and
Lidia, García-Agudo, Pilar, Aznar-Marín, Fátima, Galán-Sánchez, Pedro, García-Martos, Pilar, Marín-Casanova, Manuel, Rodríguez-Iglesias   +5 more
openaire   +2 more sources

Modelling the growth of filamentous fungi

2005
Despite the considerable industrial importance of filamentous fungi there have been very few attempts to model the complex growth process of these microorganisms. With a new generation of high performance, computerized bioreactors and new analytical techniques it is possible to obtain the necessary experimental data for setting up reliable structured ...
openaire   +2 more sources

Morphology and productivity of filamentous fungi

Applied Microbiology and Biotechnology, 2005
Cultivation processes involving filamentous fungi have been optimised for decades to obtain high product yields. Several bulk chemicals like citric acid and penicillin are produced this way. A simple adaptation of cultivation parameters for new production processes is not possible though.
L H, Grimm, S, Kelly, R, Krull, D C, Hempel   +3 more
openaire   +2 more sources

Proteome Studies of Filamentous Fungi

2011
The continued fast pace of fungal genome sequence generation has enabled proteomic analysis of a wide variety of organisms that span the breadth of the Kingdom Fungi. There is some phylogenetic bias to the current catalog of fungi with reasonable DNA sequence databases (genomic or EST) that could be analyzed at a global proteomic level.
Scott E, Baker, Ellen A, Panisko
openaire   +2 more sources