Results 111 to 120 of about 20,810 (248)

Problem areas in the use of the firefly luciferase assay for bacterial detection [PDF]

By purifying the firefly luciferase extract and adding all necessary chemicals but ATP in excess, an assay for ATP was performed by measuring the amount of light produced when a sample containing soluble ATP is added to the luciferase reaction mixture ...
Chappelle, E. W., Curtis, C. A., Knust, E. A., Picciolo, G. L., Tuttle, S. A.   +4 more
core   +1 more source

AaSIZ1‐Mediated SUMOylation of AaMYB31 Positively Regulates Freezing Tolerance in Actinidia arguta

Plant Biotechnology Journal, EarlyView.
ABSTRACT Several studies revealed that wax accumulation enhances plant tolerance to cold stress. However, the molecular regulation mechanism of wax synthesis under cold stress is still unclear in Actinidia arguta, a hardy kiwifruit species. Here, we found that cold stress significantly induces wax accumulation in Actinidia arguta leaves, which is ...
Linyao Liu, Lei Cao, Na Tang, Wangmei Ren, Zhili Zhu, Rong Wang, Minghao Xiang, Jinliang Sun, Xuan Zhang, Feng Zhang, Yunzhi Lin, Yuting Kong, Zhihui Jiang, Lihuan Wang, Yongsheng Liu, Cheng Zhang, Pengpeng Zheng   +16 more
wiley   +1 more source

Improved Red-emitting Firefly Luciferase Mutant for Biotechnical Applications [PDF]

, 2009
Luciferase from the North American firefly Photinus Pyralis catalyzes a highly efficient bioluminescent reaction that produces yellow-green light (557 nm at pH 7.8).
Davis, Audrey L
core   +1 more source

ZmWRKY29 Transcriptionally Represses ZmRBOHC to Attenuate ROS Production and Facilitates Gibberella Stalk Rot Susceptibility in Maize

Plant Biotechnology Journal, EarlyView.
ABSTRACT Gibberella stalk rot (GSR), caused by the fungal pathogen Fusarium graminearum, severely threatens maize production. However, the molecular mechanisms underlying maize resistance to GSR remain poorly understood. Here, we have identified ZmWRKY29 as a transcriptional repressor induced by F.
Jingye Fu, Li Zhu, Luyao Wang, Wenzheng Pei, Chenying Zhu, Fukun Bao, Yuhan Zhang, Gang Chen, Qiang Wang   +8 more
wiley   +1 more source

Use of Bioluminescent Imaging to Assay the Transplantation of Immortalized Human Fetal Hepatocytes into Mice

Cell Transplantation, 2008
Noninvasive serial monitoring of the fate of transplanted cells would be invaluable to evaluate the potential therapeutic use of human hepatocyte transplantation.
Moon Seok Choi, Andreea M. Catana, Jian Wu, Young Seok Kim, Sang Jeong Yoon, Alexander D. Borowsky, Sanjiv S. Gambhir, Sanjeev Gupta, Mark A. Zern   +8 more
doaj   +1 more source

Generation of Transgenic Mice to Evaluate Promoter Activity and Specificity of Two Human Endogenous Retrovirus Long Terminal Repeats = Untersuchungen zur Promotor-Aktivität und -Spezifität von zwei Long Terminal Repeats humaner endogener Retroviren in transgenen Mäusen [PDF]

, 2003
Generation of Transgenic Mice to Evaluate Promoter Activity and Specificity of two Human Endogenous Retrovirus Long Terminal Repeats Human Endogenous Retrovirus Long Terminal Repeats (HERV-LTRs) comprise 1.8% of the human genome (52.7 Mb).
Schönfeld, Regine Margarete
core  

LsMAPK6 Phosphorylates the LsCO Protein to Enhance Its Stability and Transcriptional Activity, Promoting Floral Transition Upon High Temperatures in Lettuce

Plant Biotechnology Journal, EarlyView.
ABSTRACT High temperatures significantly accelerate the timing of floral transition, namely, bolting and flowering, in lettuce, which results in severe loss of marketable yield. Thus, understanding the genetic regulation of floral transition is of great interest to plant biologists and lettuce breeders.
Tingzhen Wang, Mingjia Liu, Yufeng Tian, Qingqing Tan, Jiaxuan Wang, Huifang Mu, Chaojie Liu, Huiyu Wang, Ning Liu, Yingyan Han, Jinghong Hao   +10 more
wiley   +1 more source

pFAR plasmids: New Eukaryotic Expression Vectors for Gene Therapy, devoid of Antibiotic Resistance Markers [PDF]

, 2008
Efficient production of eukaryotic expression vectors requires the selection of plasmid-containing bacteria. To avoid the risk of dissemination of antibiotic resistance markers, we developed a new system to produce a family of plasmids Free of Antibiotic
Corinne Marie, Daniel Scherman, Gaelle Vandermeulen, Magali Richard, Mickael Quiviger, Veronique Preat   +5 more
core   +1 more source

A Bioluminescent Reporter System for Real‐Time Monitoring of the Unfolded Protein Response in Plants

Plant Biotechnology Journal, EarlyView.
ABSTRACT The unfolded protein response (UPR) is a critical mechanism for maintaining endoplasmic reticulum (ER) homeostasis under stress. Here, we developed a bioluminescent reporter system, AtbZIP60‐LUC, in Arabidopsis to dynamically monitor ER stress by coupling IRE1‐mediated splicing of bZIP60 mRNA to firefly luciferase (LUC) expression.
Yao Liu, Yue Niu, Yunxi Zhao, Wenwen Zhang, Yonglun Lv, Minqing Liu, Qian Zhang, Jiaojiao Wang, Xin Zhang, Yanjie Li, Hui Zhang, Yingying Zhang, Yan Bao   +12 more
wiley   +1 more source

Optimized Longitudinal Monitoring of Stem Cell Grafts in Mouse Brain Using a Novel Bioluminescent/Near Infrared Fluorescent Fusion Reporter

Cell Transplantation, 2017
Biodistribution and fate of transplanted stem cells via longitudinal monitoring has been successfully achieved in the last decade using optical imaging.
Laura Mezzanotte, Juvita Delancy Iljas, Ivo Que, Alan Chan, Eric Kaijzel, Rob Hoeben, Clemens Löwik   +6 more
doaj   +1 more source