Results 121 to 130 of about 21,179 (270)

Problem areas in the use of the firefly luciferase assay for bacterial detection [PDF]

By purifying the firefly luciferase extract and adding all necessary chemicals but ATP in excess, an assay for ATP was performed by measuring the amount of light produced when a sample containing soluble ATP is added to the luciferase reaction mixture ...
Chappelle, E. W., Curtis, C. A., Knust, E. A., Picciolo, G. L., Tuttle, S. A.   +4 more
core   +1 more source

Fluorescent Properties of Firefly Luciferases and Their Complexes with Luciferin

Bioscience Reports, 2000
Fluorescence of luciferases from Luciola mingrelica (single tryptophanresidue, Trp-419) and Photinus pyralis (two tryptophan residues, Trp-417,Trp-426) was studied. Analysis of quenching of tryptophan fluorescenceshowed that the tryptophan residue conserved in all luciferases is notaccessible for charged quenchers, which is explained by the presence ...
E I, Dementieva, E A, Fedorchuk, L Y, Brovko, A P, Savitskii, N N, Ugarova   +4 more
openaire   +2 more sources

ZmWRKY29 Transcriptionally Represses ZmRBOHC to Attenuate ROS Production and Facilitates Gibberella Stalk Rot Susceptibility in Maize

Plant Biotechnology Journal, EarlyView.
ABSTRACT Gibberella stalk rot (GSR), caused by the fungal pathogen Fusarium graminearum, severely threatens maize production. However, the molecular mechanisms underlying maize resistance to GSR remain poorly understood. Here, we have identified ZmWRKY29 as a transcriptional repressor induced by F.
Jingye Fu, Li Zhu, Luyao Wang, Wenzheng Pei, Chenying Zhu, Fukun Bao, Yuhan Zhang, Gang Chen, Qiang Wang   +8 more
wiley   +1 more source

Tailorable biofunctionalization of poly(acrylamide) hydrogels via firefly luciferin-bioinspired click ligation accelerates cell attachment, spreading and proliferation [PDF]

, 2023
Alexis Wolfel, Minye Jin, Nuno Araújo‐Gomes, Malin Becker, Jeroen Leijten, Liliana Moreira Teixeira, Julieta I. Paez   +6 more
openalex   +1 more source

LsMAPK6 Phosphorylates the LsCO Protein to Enhance Its Stability and Transcriptional Activity, Promoting Floral Transition Upon High Temperatures in Lettuce

Plant Biotechnology Journal, EarlyView.
ABSTRACT High temperatures significantly accelerate the timing of floral transition, namely, bolting and flowering, in lettuce, which results in severe loss of marketable yield. Thus, understanding the genetic regulation of floral transition is of great interest to plant biologists and lettuce breeders.
Tingzhen Wang, Mingjia Liu, Yufeng Tian, Qingqing Tan, Jiaxuan Wang, Huifang Mu, Chaojie Liu, Huiyu Wang, Ning Liu, Yingyan Han, Jinghong Hao   +10 more
wiley   +1 more source

A Bioluminescent Reporter System for Real‐Time Monitoring of the Unfolded Protein Response in Plants

Plant Biotechnology Journal, EarlyView.
ABSTRACT The unfolded protein response (UPR) is a critical mechanism for maintaining endoplasmic reticulum (ER) homeostasis under stress. Here, we developed a bioluminescent reporter system, AtbZIP60‐LUC, in Arabidopsis to dynamically monitor ER stress by coupling IRE1‐mediated splicing of bZIP60 mRNA to firefly luciferase (LUC) expression.
Yao Liu, Yue Niu, Yunxi Zhao, Wenwen Zhang, Yonglun Lv, Minqing Liu, Qian Zhang, Jiaojiao Wang, Xin Zhang, Yanjie Li, Hui Zhang, Yingying Zhang, Yan Bao   +12 more
wiley   +1 more source

A Microplate-Based Expression Monitoring System for Arabidopsis NITRATE TRANSPORTER2.1 Using the Luciferase Reporter

Bio-Protocol
Gene expression analysis is a fundamental technique to elucidate the regulatory mechanisms of genes of interest or to reveal the patterns of plant response to environmental stimuli.
Yoshiaki Ueda, Shuichi Yanagisawa
doaj   +1 more source

Use of Bioluminescent Imaging to Assay the Transplantation of Immortalized Human Fetal Hepatocytes into Mice

Cell Transplantation, 2008
Noninvasive serial monitoring of the fate of transplanted cells would be invaluable to evaluate the potential therapeutic use of human hepatocyte transplantation.
Moon Seok Choi, Andreea M. Catana, Jian Wu, Young Seok Kim, Sang Jeong Yoon, Alexander D. Borowsky, Sanjiv S. Gambhir, Sanjeev Gupta, Mark A. Zern   +8 more
doaj   +1 more source

Evaluation of Bioluminescence Activity of Firefly Luciferin Nucleotide Derivatives [PDF]

, 2012
Satoshi Iwano, Satoshi Kojima, Takashi Hirano, Shôjirô Maki, Haruki Niwa   +4 more
openalex   +1 more source

The Transcription Factor OsWRKY64 Interacts With OsART1 to Positively Regulate Al Resistance in Rice

Plant Biotechnology Journal, EarlyView.
A proposed working model illustrating the cooperative regulation of Al tolerance by OsWRKY64 and OsART1 in rice. Under Al stress conditions in acidic soil, the transcription factor OsART1 directly binds to the promoter of OsWRKY64 to activate its transcription. The synthesized OsWRKY64 protein then physically interacts with OsART1 in the nucleus.
Changzhao Chen, Qiang Zhang, Yonggang Xu, Jibo Yang, Jing Yan, Mengmeng Jiang, Lu Zheng, Tingwu Liu, Jiu Huang, Renfang Shen, Xiaofang Zhu   +10 more
wiley   +1 more source