Results 81 to 90 of about 83,026 (211)
A Microfluidic Device for Massively Parallel, Whole-lifespan Imaging of Single Fission Yeast Cells
Whole-lifespan single-cell analysis has greatly increased our understanding of fundamental cellular processes such as cellular aging. To observe individual cells across their entire lifespan, all progeny must be removed from the growth medium, typically ...
Stephen Jones Jr +3 more
doaj +1 more source
A novel assay evaluates plant biostimulants targeting the brassinosteroid receptor BRI1, a central component in plant growth and development. By expressing BRI1 in fission yeast, functional activation by brassinolide (BL) was enabled. Transcriptomic profiling identified BL‐responsive genes, leading to a GFP‐based reporter system. This platform offers a
Maribel Marquina +9 more
wiley +1 more source
Regulation of polarised growth in fungi [PDF]
Polarised growth in fungi occurs through the delivery of secretory vesicles along tracks formed by cytoskeletal elements to specific sites on the cell surface where they dock with a multiprotein structure called the exocyst before fusing with the ...
Adamo +112 more
core +1 more source
Design principles of Cdr2 node patterns in fission yeast cells [PDF]
Hannah E. Opalko +4 more
openalex +1 more source
Highly divergent apicomplexan cytoskeletons provide additional models for actin biology
Actin is a highly conserved, abundant and central molecule in eukaryotes. Apicomplexans contain some of the most sequence‐divergent actin cytoskeletons known. This divergence results in overlapping yet different biochemical properties. Here, we highlight the remarkable divergence of the actin cytoskeleton by comparing apicomplexan to classical systems,
Yukino Kobayashi, Ross G. Douglas
wiley +1 more source
Preferential attachment in the protein network evolution
The Saccharomyces cerevisiae protein-protein interaction map, as well as many natural and man-made networks, shares the scale-free topology. The preferential attachment model was suggested as a generic network evolution model that yields this universal ...
A. Vazquez +8 more
core +1 more source
Structural plasticity of the living kinetochore [PDF]
The kinetochore is a large, evolutionarily conserved protein structure that connects chromosomes with microtubules. During chromosome segregation, outer kinetochore components track depolymerizing ends of microtubules to facilitate the separation of ...
Akiyoshi +90 more
core +1 more source
External apodization phase‐contrast (ExAPC) microscopy enables label‐free visualization of diverse intracellular structures in living mammalian cells. By enhancing phase‐contrast imaging through optical apodization, ExAPC reveals fine subcellular details, such as lipid droplets and organelle contacts, without fluorescent labels.
Hiroshi Ohno +19 more
wiley +1 more source
ABSTRACT Aim We investigated the biogeographic consequences of chromosomal speciation, or structural changes in chromosome arrangement that lead to reproductive isolation, an intriguing speciation mechanism with implications for the phylogenetic and geographic distribution of species and chromosomal diversity. Location. North and Central America. Taxa.
Isaac W. Krone, Erin P. Westeen
wiley +1 more source
Background Each of the three individual components of the CMG complex (Cdc45, MCM and GINS) is essential for chromosomal DNA replication in eukaryotic cells, both for the initiation of replication at origins and also for normal replication fork ...
MacNeill Stuart A, Akman Gökhan
doaj +1 more source

