Results 81 to 90 of about 83,026 (211)

A Microfluidic Device for Massively Parallel, Whole-lifespan Imaging of Single Fission Yeast Cells

open access: yesBio-Protocol, 2018
Whole-lifespan single-cell analysis has greatly increased our understanding of fundamental cellular processes such as cellular aging. To observe individual cells across their entire lifespan, all progeny must be removed from the growth medium, typically ...
Stephen Jones Jr   +3 more
doaj   +1 more source

Biostimulants in plant brassinosteroid hormone receptor BRI1 activation—a new system to evaluate activation capacity

open access: yesThe FEBS Journal, EarlyView.
A novel assay evaluates plant biostimulants targeting the brassinosteroid receptor BRI1, a central component in plant growth and development. By expressing BRI1 in fission yeast, functional activation by brassinolide (BL) was enabled. Transcriptomic profiling identified BL‐responsive genes, leading to a GFP‐based reporter system. This platform offers a
Maribel Marquina   +9 more
wiley   +1 more source

Regulation of polarised growth in fungi [PDF]

open access: yes, 2008
Polarised growth in fungi occurs through the delivery of secretory vesicles along tracks formed by cytoskeletal elements to specific sites on the cell surface where they dock with a multiprotein structure called the exocyst before fusing with the ...
Adamo   +112 more
core   +1 more source

Design principles of Cdr2 node patterns in fission yeast cells [PDF]

open access: green, 2023
Hannah E. Opalko   +4 more
openalex   +1 more source

Highly divergent apicomplexan cytoskeletons provide additional models for actin biology

open access: yesThe FEBS Journal, EarlyView.
Actin is a highly conserved, abundant and central molecule in eukaryotes. Apicomplexans contain some of the most sequence‐divergent actin cytoskeletons known. This divergence results in overlapping yet different biochemical properties. Here, we highlight the remarkable divergence of the actin cytoskeleton by comparing apicomplexan to classical systems,
Yukino Kobayashi, Ross G. Douglas
wiley   +1 more source

Preferential attachment in the protein network evolution

open access: yes, 2003
The Saccharomyces cerevisiae protein-protein interaction map, as well as many natural and man-made networks, shares the scale-free topology. The preferential attachment model was suggested as a generic network evolution model that yields this universal ...
A. Vazquez   +8 more
core   +1 more source

Structural plasticity of the living kinetochore [PDF]

open access: yes, 2017
The kinetochore is a large, evolutionarily conserved protein structure that connects chromosomes with microtubules. During chromosome segregation, outer kinetochore components track depolymerizing ends of microtubules to facilitate the separation of ...
Akiyoshi   +90 more
core   +1 more source

Label‐free imaging of intracellular structures in living mammalian cells via external apodization phase‐contrast microscopy

open access: yesThe FEBS Journal, EarlyView.
External apodization phase‐contrast (ExAPC) microscopy enables label‐free visualization of diverse intracellular structures in living mammalian cells. By enhancing phase‐contrast imaging through optical apodization, ExAPC reveals fine subcellular details, such as lipid droplets and organelle contacts, without fluorescent labels.
Hiroshi Ohno   +19 more
wiley   +1 more source

Does Karyotype Structure Co‐Occurrence Patterns in a Chromosomally Diverse and Species‐Rich Clade of Lizards (Genus Sceloporus)?

open access: yesJournal of Biogeography, EarlyView.
ABSTRACT Aim We investigated the biogeographic consequences of chromosomal speciation, or structural changes in chromosome arrangement that lead to reproductive isolation, an intriguing speciation mechanism with implications for the phylogenetic and geographic distribution of species and chromosomal diversity. Location. North and Central America. Taxa.
Isaac W. Krone, Erin P. Westeen
wiley   +1 more source

MCM-GINS and MCM-MCM interactions in vivo visualised by bimolecular fluorescence complementation in fission yeast

open access: yesBMC Cell Biology, 2009
Background Each of the three individual components of the CMG complex (Cdc45, MCM and GINS) is essential for chromosomal DNA replication in eukaryotic cells, both for the initiation of replication at origins and also for normal replication fork ...
MacNeill Stuart A, Akman Gökhan
doaj   +1 more source

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