Results 81 to 90 of about 592,269 (288)

Targeted amino-terminal acetylation of recombinant proteins in E. coli. [PDF]

, 2010
One major limitation in the expression of eukaryotic proteins in bacteria is an inability to post-translationally modify the expressed protein. Amino-terminal acetylation is one such modification that can be essential for protein function.
Arthur T. Coulton, Geeves, Michael A., Couton, Arthur T., Johnson Matthew, Mulvihill Daniel P., Johnson, Matthew, Daniel P. Mulvihill, Matthew Johnson, Mulvihill, Daniel P., Coulton Arthur T., Daniel P Mulvihill, Michael A Geeves, Arthur T Coulton, Michael A. Geeves, Geeves Michael A.   +14 more
core   +1 more source

Understanding the molecular mechanisms of human diseases: the benefits of fission yeasts

Microbial Cell
The role of model organisms such as yeasts in life science research is crucial. Although the baker’s yeast (Saccharomyces cerevisiae) is the most popular model among yeasts, the contribution of the fission yeasts (Schizosaccharomyces) to life science is ...
Lajos Acs-Szabo, Laszlo Attila Papp, Ida Miklos   +2 more
doaj   +1 more source

Comparative Research: Regulatory Mechanisms of Ribosomal Gene Transcription in Saccharomyces cerevisiae and Schizosaccharomyces pombe

Biomolecules, 2023
Restricting ribosome biosynthesis and assembly in response to nutrient starvation is a universal phenomenon that enables cells to survive with limited intracellular resources.
Hayato Hirai, Kunihiro Ohta
doaj   +1 more source

Schizosaccharomyces japonicus: the fission yeast is a fusion of yeast and hyphae

Yeast, 2014
The clade of Schizosaccharomyces includes 4 species: S. pombe, S. octosporus, S. cryophilus, and S. japonicus. Although all 4 species exhibit unicellular growth with a binary fission mode of cell division, S. japonicus alone is dimorphic yeast, which can
H. Niki
semanticscholar   +1 more source

The ageing holobiont: crosstalk between telomere dynamics, oxidative stress and the gut microbiome

Biological Reviews, EarlyView.
ABSTRACT The gut tissue is at the frontline of early onset of ageing. It exhibits high cell turnover rates and rapid telomere shortening, which can have systemic effects on the developing or senescing organism. We conducted a literature review of studies on the crosstalk between telomere length dynamics, telomerase activity, oxidative stress, and gut ...
Michael L. Pepke, Søren B. Hansen, Morten T. Limborg   +2 more
wiley   +1 more source

The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function [PDF]

, 2005
During mitosis, the spindle assembly checkpoint (SAC) responds to faulty attachments between kinetochores and the mitotic spindle by imposing a metaphase arrest until the defect is corrected, thereby preventing chromosome missegregation. A genetic screen
Vanoosthuyse, V., Hardwick, Kevin, Kadura, Sheila, Sazer, Shelley, He, Xiangwei, Vanoosthuyse, Vincent, Hardwick, Kevin G   +6 more
core   +1 more source

Analysis of DNA Metabolism in Fission Yeast [PDF]

Cold Spring Harbor Protocols, 2017
The fission yeast Schizosaccharomyces pombe is an excellent model organism to study DNA metabolism, in which the DNA replication and repair mechanisms are evolutionarily conserved. In this introduction we describe a range of methods commonly used to study aspects of DNA metabolism in fission yeast, focusing on approaches used for the analysis of genome
Antequera, Francisco, Humphrey, Timothy C.   +1 more
openaire   +4 more sources

Extensive mass spectrometry-based analysis of the fission yeast proteome: the Schizosaccharomyces pombe PeptideAtlas

, 2013
We report a high quality and system-wide proteome catalogue covering 71% (3,542 proteins) of the predicted genes of fission yeast, Schizosaccharomyces pombe, presenting the largest protein dataset to date for this important model organism.
Schmidt, A, Lössner, C, Gunaratne, J, Blackstock, W, Xia, Rachel Li Hai, Ahrné, Erik, Quandt, A, Neo, Suat Peng, Saraç, OS, Gracia, Tannia, Gracia, T, Xia, RL, Bähler, J, Bähler, Jürg, Tan, KH, Tan, Keng Hwa, Aebersold, Ruedi, Beyer, A, Beyer, Andreas, Loguercio, Salvatore, Quandt, Andreas, Gunaratne, Jayantha, Aebersold, R, Blackstock, Walter, Loguercio, S, Saraç, Omer Sinan, Neo, SP, Schmidt, Alexander, Ahrné, E, Lössner, Christopher   +29 more
core   +1 more source

Implementation of the CRISPR-Cas9 system in fission yeast

Nature Communications, 2014
Application of the CRISPR-Cas9 genome editing system in the model organism Schizosaccharomyces pombe has been hampered by the lack of constructs to express RNA of arbitrary sequence.
Jake Z. Jacobs, Keith M. Ciccaglione, Vincent Tournier, M. Zaratiegui   +3 more
semanticscholar   +1 more source

Cost‐Effective Solid‐State NMR Of Fungal Glucans: A Case Study On Schizosaccharomyces pombe

Chemistry – A European Journal, EarlyView.
A cost‐effective solid‐state NMR strategy enables replication and semi‐quantitative assessment of inherent variation of the fungal cell wall. Whole cell and cell wall extractions yield subtly different compositional differences, revealing molecular adaptations invisible to microscopy.
Ananya Singh, Teresa Massam‐Wu, Mohan Balasubramanian, Wing Ying Chow   +3 more
wiley   +1 more source