Results 71 to 80 of about 126,813 (257)
Insecticide Resistance and Fitness Cost
, 2016 The intensive use of chemicals through decades has been selecting resistant popula‐ tions of several insect species to distinct classes of insecticides, like neurotoxics, in‐ sect growth regulators, and toxins derived from bacteria. Insecticide resistance is nowadays a huge challenge for control programs of pests of rural practices and principally to ...
Thiago Affonso Belinato +1 more
openaire +2 more sources
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A Cost of Sexual Attractiveness to High-Fitness Females
PLoS Biology, 2009 Adaptive mate choice by females is an important component of sexual selection in many species. The evolutionary consequences of male mate preferences, however, have received relatively little study, especially in the context of sexual conflict, where males often harm their mates.
Tristan A F Long +3 more
openaire +4 more sources
Rifampicin Resistance: Fitness Costs and the Significance of Compensatory Evolution [PDF]
Antibiotics, 2013 Seventy years after the introduction of antibiotic chemotherapy to treat tuberculosis, problems caused by drug-resistance in Mycobacterium tuberculosis have become greater than ever. The discovery and development of novel drugs and drug combination therapies will be critical to managing these problematic infections.
Hughes, Diarmaid, Brandis, Gerrit
openaire +4 more sources
FEBS Open Bio, EarlyView.This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
Fitness Cost of Chromosomal Drug Resistance-Conferring Mutations [PDF]
Antimicrobial Agents and Chemotherapy, 2002 ABSTRACTTo study the cost of chromosomal drug resistance mutations to bacteria, we investigated the fitness cost of mutations that confer resistance to different classes of antibiotics affecting bacterial protein synthesis (aminocyclitols, 2-deoxystreptamines, macrolides).
Sander, Peter +6 more
openaire +3 more sources
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Engineering Reports, 2020 Bacterial regulatory small RNAs (sRNAs) have shown promise for gene knock‐down studies and metabolic engineering. However, some mRNAs might be difficult to target due to poor binding by the Hfq chaperone, individual synthetic sRNAs can have off‐target ...
Carolin Vogel +5 more
doaj +1 more source
Balancing the fit and logistics costs of market segmentations
European Journal of Operational Research, 2011 Segments are typically formed to serve distinct groups of consumers with differentiated marketing mixes, that better fit their specific needs and wants. However, buyers in a segment are not necessarily geographically closely located. Serving a geographically dispersed segment with one marketing mix can increase the logistics costs in the form of high ...
Turkensteen, Marcel +2 more
openaire +1 more source