Results 1 to 10 of about 44,095 (208)

Flavoprotein monooxygenases for oxidative biocatalysis: recombinant expression in microbial hosts and applications [PDF]

Frontiers in Microbiology, 2014
External flavoprotein monooxygenases comprise a group of flavin-dependent oxidoreductases that catalyze the insertion of one atom of molecular oxygen into an organic substrate and the second atom is reduced to water. These enzymes are involved in a great
Romina D. Ceccoli, Dario A. Bianchi, Daniela V. Rial   +2 more
doaj   +4 more sources

Activity assays for flavoprotein oxidases: an overview [PDF]

Applied Microbiology and Biotechnology
Flavoprotein oxidases have found many biotechnological applications. For identifying and improving their characteristics, it is essential to have reliable and robust assay methodology available. The methodologies used to monitor their activity seem to be
Lars L. Santema, Marco W. Fraaije
doaj   +2 more sources

Can flavoprotein monooxygenases functionalize long-chain n-alkanes? [PDF]

PLoS ONE
Long-chain n-alkane functionalization is relevant for industrial and environmental applications, but it remains a significant challenge due to the inherent stability of their covalent bonds. Biocatalytic approaches offer promising strategies due to their
Raul Mireles, Arne Matthews, Robin Teufel, Lianet Noda-García   +3 more
doaj   +2 more sources

The structural and functional roles of the flavin cofactor FAD in mammalian cryptochromes

Frontiers in Molecular Biosciences, 2023
The importance of circadian rhythms in human health and disease calls for a thorough understanding of the underlying molecular machinery, including its key components, the flavin adenine dinucleotide (FAD)-containing flavoproteins cryptochrome 1 and 2 ...
Giulia Calloni, R. Martin Vabulas
doaj   +1 more source

Yin and Yang in Post-Translational Modifications of Human D-Amino Acid Oxidase

Frontiers in Molecular Biosciences, 2021
In the central nervous system, the flavoprotein D-amino acid oxidase is responsible for catabolizing D-serine, the main endogenous coagonist of N-methyl-D-aspartate receptor.
Silvia Sacchi, Valentina Rabattoni, Matteo Miceli, Loredano Pollegioni   +3 more
doaj   +1 more source

Substrate Inhibition of 5β-Δ4-3-Ketosteroid Dehydrogenase in Sphingobium sp. Strain Chol11 Acts as Circuit Breaker During Growth With Toxic Bile Salts

Frontiers in Microbiology, 2021
In contrast to many steroid hormones and cholesterol, mammalian bile salts are 5β-steroids, which leads to a bent structure of the steroid core. Bile salts are surface-active steroids excreted into the environment in large amounts, where they are subject
Franziska M. Feller, Gina Marke, Steffen L. Drees, Lars Wöhlbrand, Ralf Rabus, Bodo Philipp, Bodo Philipp   +6 more
doaj   +1 more source

Redox-controlled reorganization and flavin strain within the ribonucleotide reductase R2b–NrdI complex monitored by serial femtosecond crystallography

eLife, 2022
Redox reactions are central to biochemistry and are both controlled by and induce protein structural changes. Here, we describe structural rearrangements and crosstalk within the Bacillus cereus ribonucleotide reductase R2b–NrdI complex, a di-metal ...
Juliane John, Oskar Aurelius, Vivek Srinivas, Patricia Saura, In-Sik Kim, Asmit Bhowmick, Philipp S Simon, Medhanjali Dasgupta, Cindy Pham, Sheraz Gul, Kyle D Sutherlin, Pierre Aller, Agata Butryn, Allen M Orville, Mun Hon Cheah, Shigeki Owada, Kensuke Tono, Franklin D Fuller, Alexander Batyuk, Aaron S Brewster, Nicholas K Sauter, Vittal K Yachandra, Junko Yano, Ville RI Kaila, Jan Kern, Hugo Lebrette, Martin Högbom   +26 more
doaj   +1 more source

Structural Determinants of the Specific Activities of an L-Amino Acid Oxidase from Pseudoalteromonas luteoviolacea CPMOR-1 with Broad Substrate Specificity

Molecules, 2022
The Pseudoalteromonas luteoviolacea strain CPMOR-1 expresses a flavin adenine dinucleotide (FAD)-dependent L-amino acid oxidase (LAAO) with broad substrate specificity.
Kyle J. Mamounis, Maria Luiza Caldas Nogueira, Daniela Priscila Marchi Salvador, Andres Andreo-Vidal, Antonio Sanchez-Amat, Victor L. Davidson   +5 more
doaj   +1 more source

Identification of a Novel Self-Sufficient Styrene Monooxygenase from Rhodococcus opacus 1CP. [PDF]

, 2009
Sequence analysis of a 9-kb genomic fragment of the actinobacterium Rhodococcus opacus 1CP led to identification of an open reading frame encoding a novel fusion protein, StyA2B, with a putative function in styrene metabolism via styrene oxide and ...
Berkel, W.J.H., van, Eulberg, D., Kaschabek, S.R., Lakner, S., Schloemann, M., Tischler, D.   +5 more
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Fusarium Photoreceptors

Journal of Fungi, 2023
Light is an important modulating signal in fungi. Fusarium species stand out as research models for their phytopathogenic activity and their complex secondary metabolism.
Javier Pardo-Medina, M. Carmen Limón, Javier Avalos   +2 more
doaj   +1 more source