Results 21 to 30 of about 29,105 (237)

EPR on Flavoproteins [PDF]

, 2014
Flavoproteins often employ radical mechanisms in their enzymatic reactions. This involves paramagnetic species, which can ideally be investigated with electron paramagnetic resonance (EPR) spectroscopy. In this chapter we focus on the example of flavin-based photoreceptors and discuss, how different EPR methods have been used to extract information ...
Richard, Brosi, Robert, Bittl, Christopher, Engelhard   +2 more
openaire   +2 more sources

Biosynthesis of flavoproteins [PDF]

, 1987
published
Yagi, Kunio, Inagaki, Taisuke, Ichihara, Chikako, Koshizaka, Takuya, Ohishi, Nobuko, Tsukagoshi, Norihiro, Udaka, Shigezo, Nishikimi, Morimitsu, Ozawa, Takayuki, Ghisla, Sandro   +9 more
openaire   +2 more sources

Spin Dynamics of Flavoproteins

International Journal of Molecular Sciences, 2023
This short review reports the surprising phenomenon of nuclear hyperpolarization occurring in chemical reactions, which is called CIDNP (chemically induced dynamic nuclear polarization) or photo-CIDNP if the chemical reaction is light-driven. The phenomenon occurs in both liquid and solid-state, and electron transfer systems, often carrying flavins as ...
Jörg Matysik, Luca Gerhards, Tobias Theiss, Lisa Timmermann, Patrick Kurle-Tucholski, Guzel Musabirova, Ruonan Qin, Frank Ortmann, Ilia A. Solov’yov, Tanja Gulder   +9 more
openaire   +2 more sources

Express your LOV: an engineered flavoprotein as a reporter for protein expression and purification [PDF]

, 2012
In this work, we describe the utility of Light, Oxygen, or Voltage-sensing (LOV) flavoprotein domains from plant phototropins as a reporter for protein expression and function.
Jayde A Gawthorne, Akpunarlieva, S.N., John M. Christie (107410), Beckham, K.S.H., Gabrielsen, M., Katherine S. H. Beckham (107407), Alto, Neal M., Alto, N.M., Christie, J.M., Snezhana N. Akpunarlieva (107403), Jayde A. Gawthorne (107393), Mads Gabrielsen (107416), Reddick, L.E., Akpunarlieva, Snezhana N., Jayde A. Gawthorne, Roe, A.J., Katherine S H Beckham, Christie, John M., Snezhana N. Akpunarlieva, L. Evan Reddick (107398), Beckham, Katherine S. H., Mads Gabrielsen, Andrew J Roe, John M Christie, Gabrielsen, Mads, Gawthorne, J.A., Reddick, L. Evan, Andrew J. Roe, Neal M. Alto (107413), John M. Christie, L. Evan Reddick, Snezhana N Akpunarlieva, Neal M. Alto, Andrew J. Roe (107419), Neal M Alto, Roe, Andrew J., Katherine S. H. Beckham, L Evan Reddick, Gawthorne, Jayde A.   +38 more
core   +1 more source

Functional characterization and stability improvement of a ‘thermophilic-like’ ene-reductase from Rhodococcus opacus 1CP

Frontiers in Microbiology, 2015
Ene-reductases are widely applied for the asymmetric synthesis of relevant industrial chemicals. A novel ene-reductase OYERo2 was found within a set of 14 putative Old Yellow Enzymes (OYEs) obtained by genome mining of the actinobacterium Rhodococcus ...
Anika eRiedel, Anika eRiedel, Marika eMehnert, Caroline E. Paul, Adrie H. Westphal, Willem JH Van Berkel, Dirk eTischler, Dirk eTischler   +7 more
doaj   +1 more source

3-Hydroxybenzoate 6-Hydroxylase from Rhodococcus jostii RHA1 Contains a Phosphatidylinositol Cofactor

Frontiers in Microbiology, 2017
3-Hydroxybenzoate 6-hydroxylase (3HB6H, EC 1.13.14.26) is a FAD-dependent monooxygenase involved in the catabolism of aromatic compounds in soil microorganisms. 3HB6H is unique among flavoprotein hydroxylases in that it harbors a phospholipid ligand. The
Stefania Montersino, Evelien te Poele, Roberto Orru, Adrie H. Westphal, Arjan Barendregt, Albert J. R. Heck, Robert van der Geize, Lubbert Dijkhuizen, Andrea Mattevi, Willem J. H. van Berkel   +9 more
doaj   +1 more source

Catalytic Performance of a Class III Old Yellow Enzyme and Its Cysteine Variants

Frontiers in Microbiology, 2018
Class III old yellow enzymes (OYEs) contain a conserved cysteine in their active sites. To address the role of this cysteine in OYE-mediated asymmetric synthesis, we have studied the biocatalytic properties of OYERo2a from Rhodococcus opacus 1CP (WT) as ...
Anika Scholtissek, Eric Gädke, Eric Gädke, Caroline E. Paul, Caroline E. Paul, Adrie H. Westphal, Willem J. H. van Berkel, Dirk Tischler   +7 more
doaj   +1 more source

CHOP THERAPY INDUCED MITOCHONDRIAL REDOX STATE ALTERATION IN NON-HODGKIN'S LYMPHOMA XENOGRAFTS [PDF]

Journal of Innovative Optical Health Sciences, 2013
We are interested in investigating whether cancer therapy may alter the mitochondrial redox state in cancer cells to inhibit their growth and survival.
H. N. XU, H. ZHAO, T. A. MIR, S. C. LEE, M. FENG, R. CHOE, J. D. GLICKSON, L. Z. LI   +7 more
doaj   +1 more source

A Cysteine Pair Controls Flavin Reduction by Extracellular Cytochromes during Anoxic/Oxic Environmental Transitions

mBio, 2023
Many bacteria of the genus Shewanella are facultative anaerobes able to reduce a broad range of soluble and insoluble substrates, including Fe(III) mineral oxides.
Michael P. Norman, Marcus J. Edwards, Gaye F. White, Joshua A. J. Burton, Julea N. Butt, David J. Richardson, Ricardo O. Louro, Catarina M. Paquete, Thomas A. Clarke   +8 more
doaj   +1 more source

Flavoprotein Monooxygenases and Halogenases

, 2021
In this book chapter, we give an overview of flavoprotein monooxygenases (FPMOs) as a guideline for applied biocatalysis. First, we introduce the FPMO field and briefly explain the various reaction mechanisms.
Caroline E. Paul, Paul, C.E., Alice Guarneri, Willem J.H. Berkel, Guarneri, A., van Berkel, W.J.H.   +5 more
core   +1 more source